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Registros recuperados : 38 | |
1. | | MATSUBARA, F. H.; CRUZ, N. A.; LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F. Histoquímica em hemócitos de larvas de Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae) suscetíveis e resistentes ao AgMNPV. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1314-1. Biblioteca(s): Embrapa Soja. |
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5. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. Is the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 293. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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6. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A.; TOLEDO, L. A. Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae). Neotropical Entomology, Londrina, v. 33, n. 4, p. 427-431, July/Aug. 2004. Biblioteca(s): Embrapa Soja. |
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8. | | LEVY, S. M.; FALLEIROS, A. M. F.; BASSO, N. A.; MOSCARDI, F.; GREGÓRIO, E. A. Morfologia do intestino médio de Anticarsia gemmatalis, susceptível e resistente ao vírus da poliedrose nuclear (AgMNPV), em larvas controle e infectadas pelo virus. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 9., 2005, Recife. Anais... Recife, 2005. p. 188. Biblioteca(s): Embrapa Soja. |
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9. | | LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F.; SILVA, R. J.; GREGÓRIO, E. A. Morfometria do epitélio do intestino médio de Anticarsia gemmatalis (Hübner,1818): estudo comparativo entre larvas suscetíveis e resistentes ao AgMNPV. In: SICONBIOL, 10., 2007, Brasília, DF. Inovar para preservar a vida: [palestras e resumos]. Brasília, DF: Sociedade Entomológica do Brasil, 2007. 1 CD-ROM. Resumo pdf. 102. Biblioteca(s): Embrapa Soja. |
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12. | | MATSUBARA, F. H.; MARQUES, C. R. G.; LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F. Estudo dos hemócitos de larvas de Anticarsia gemmtalis (Hübner, 1818) (Lepidoptera: Noctuidae) suscetíveis e resistentes ao AgMNPV à microscopia eletrônica de varredura. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1314-2. Biblioteca(s): Embrapa Soja. |
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13. | | CRUZ, N. A.; HOFFMANN-CAMPO, C. B.; LEVY, S. M.; FALLEIROS, A. M. F. Efeitos de variedades de soja no intestino médio de lagartas de Anticarsia gemmatalis (Hübner,1818) (Lepidoptera: Erebidae) resistentes ao vírus AgMNPV. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., Maceio, 2016. Anais... Brasília, DF: Embrapa, 2016. p. 128. Editores técnicos: Elio Cesar Guzzo, Marcus Vinicius Sampaio, Jader Braga Maia, Aldomário Santo Negrisoli Junior. Biblioteca(s): Embrapa Soja. |
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14. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A.; TOLEDO, L. A. Ultramorphology of digestive tract of Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae) at final larval development. Semina: Ciências Agrárias, Londrina, v. 29, n. 2, p. 313-322, abr./jun. 2008. Biblioteca(s): Embrapa Soja. |
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15. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; ANDRADE, F. G.; GREGÓRIO, E. A. Ultrastructure of the endocrine cels in the midgut of Anticarsia gemmatalis (Lepidoptera) larvae. Brazilian Journal of Morphological Sciences, São Paulo, v. 22, p. 189, 2005. Supplement. Edição dos Resumos do XX Congress of the Brazilian Society for Microscopy and Microanalysis, Águas de Lindóia, SP, Agosto, 2005. Biblioteca(s): Embrapa Soja. |
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18. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. Differences in the peritrophic membrane of susceptible and resistant Anticarsia gemmatalis larvae to the insect nucleopolyhedrovirus (AgMNPV). In: INTERNATIONAL COLLOQUIUM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 8.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS 6.; ANNUAL MEETING OF THE SIP, 35., 2002, Foz do Iguassu. Program and abstracts. Londrina: Embrapa Soja: Embrapa Recursos Genéticos e Biotecnologia: UEL: SIP, 2002. p. 43-44. (Embrapa Soja. Documentos, 183; Embrapa Recursos Genéticos e Biotecnologia, 73). Biblioteca(s): Embrapa Soja. |
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20. | | CARVALHO, R. B. R.; MATSUBARA, F. H.; MARQUES, C. R. G.; MOSCARDI, F.; LEVY, S. M.; FALLEIROS, A. M. F. Histologia do corpo gorduroso de larvas de Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1619-1. Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 38 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
07/04/2004 |
Data da última atualização: |
27/07/2007 |
Autoria: |
LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. |
Título: |
Is the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
Páginas: |
p. 293. |
Série: |
(Embrapa Soja. Documentos, 228). |
Idioma: |
Inglês |
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
Conteúdo: |
Anticarsia gemmatalis is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increase use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that midgut is considered one of the most important barriers against viral invasion, before systemic infection can be caused in various tissues if the virus succeeds in reaching the host larvae hemocele. Our work aim to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 oclusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cells were the most affected among the different midgut epithelial cells. In the SL, this cells shows many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However in RL, this cells exhibited minor morphological damages up to 120 hours. Polyhedra were visualized in the midgut of both SL and RL from 96 hours of infection, but the amount of viral structure were always higher in SL, affecting the midgut epithelial cells and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invade RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of RL. The mechanism interfering with the virus infection progress in the midgut cells of RL is still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq). MenosAnticarsia gemmatalis is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increase use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that midgut is considered one of the most important barriers against viral invasion, before systemic infection can be caused in various tissues if the virus succeeds in reaching the host larvae hemocele. Our work aim to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 oclusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cells were the most affected among the different midgut epithelial cells. In the SL, this cells shows many morphological signs of cellular... Mostrar Tudo |
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LEADER 03244naa a2200193 a 4500 001 1466838 005 2007-07-27 008 2004 bl uuuu u00u1 u #d 100 1 $aLEVY, S. M. 245 $aIs the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? 260 $c2004 300 $ap. 293. 490 $a(Embrapa Soja. Documentos, 228). 500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. 520 $aAnticarsia gemmatalis is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increase use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that midgut is considered one of the most important barriers against viral invasion, before systemic infection can be caused in various tissues if the virus succeeds in reaching the host larvae hemocele. Our work aim to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 oclusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cells were the most affected among the different midgut epithelial cells. In the SL, this cells shows many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However in RL, this cells exhibited minor morphological damages up to 120 hours. Polyhedra were visualized in the midgut of both SL and RL from 96 hours of infection, but the amount of viral structure were always higher in SL, affecting the midgut epithelial cells and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invade RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of RL. The mechanism interfering with the virus infection progress in the midgut cells of RL is still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq). 700 1 $aFALLEIROS, A. M. F. 700 1 $aMOSCARDI, F. 700 1 $aGREGÓRIO, E. A. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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