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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/03/2005 |
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Data da última atualização: |
23/03/2005 |
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Autoria: |
FORATO, L. A.; BICUDO, T. C.; COLNAGO, L. A. |
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Título: |
Conformation of alfa Zeins in solid state by fourier Transform IR. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Biopolymers (Biospectroscopy), v. 72, 421-426, 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: The major maize storage proteins (alpha zeins) are deposited as an insoluble mass in the protein bodies of the endosperm. Because they are insoluble in water, most structural studies are performed in alcohol solutions. To solve the question raised by several authors about denaturation of the a zein structure by alcohol, we analyze the secondary structure of a zeins prepared with and without solubilization in alcohol (corn gluten meal and protein bodies with high concentrations of a zeins and traces of beta zeins). The secondary structures of a zeins are analyzed in the solid state by Fourier transform IR spectroscopy (FTIR) in KBr penets and solid-state 13C-NMR spectroscopy. The proportion of secondary structures obtained by FTIR of a zeins prepared with and without solubilization in alcohol yield almost identical proportions of a helices and (3 sheets. The proportion of a helices (43%) agrees with that measured by circular dichroism in an alcohol solution. However, the proportion of beta sheets (28%) is higher than the one measured by the same technique. Gluten and protein body samples with high beta zein content showed higher beta sheet and lower a helix proportions than that obtained for a zein preparations. The solid-state 13C-NMR spectra show the carbonyl peak for the a zeins at Delta 176 and for the sample rich in beta zeins at gama 172, which demonstrares the presence of a high C9ntent of a helices and beta sheets, respectively. These results indicare that alcohol solubilization does not affect the conformation of a zeins, validating the secondary structure measurements in solution. MenosABSTRACT: The major maize storage proteins (alpha zeins) are deposited as an insoluble mass in the protein bodies of the endosperm. Because they are insoluble in water, most structural studies are performed in alcohol solutions. To solve the question raised by several authors about denaturation of the a zein structure by alcohol, we analyze the secondary structure of a zeins prepared with and without solubilization in alcohol (corn gluten meal and protein bodies with high concentrations of a zeins and traces of beta zeins). The secondary structures of a zeins are analyzed in the solid state by Fourier transform IR spectroscopy (FTIR) in KBr penets and solid-state 13C-NMR spectroscopy. The proportion of secondary structures obtained by FTIR of a zeins prepared with and without solubilization in alcohol yield almost identical proportions of a helices and (3 sheets. The proportion of a helices (43%) agrees with that measured by circular dichroism in an alcohol solution. However, the proportion of beta sheets (28%) is higher than the one measured by the same technique. Gluten and protein body samples with high beta zein content showed higher beta sheet and lower a helix proportions than that obtained for a zein preparations. The solid-state 13C-NMR spectra show the carbonyl peak for the a zeins at Delta 176 and for the sample rich in beta zeins at gama 172, which demonstrares the presence of a high C9ntent of a helices and beta sheets, respectively. These results indicare that a... Mostrar Tudo |
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Palavras-Chave: |
alfa Zeins; Conformação; Estado sólido; Estrutura Secundárias; NMR; Spectroscopia IR Fourier. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02217naa a2200217 a 4500
001 1028875
005 2005-03-23
008 2003 bl --- 0-- u #d
100 1 $aFORATO, L. A.
245 $aConformation of alfa Zeins in solid state by fourier Transform IR.
260 $c2003
520 $aABSTRACT: The major maize storage proteins (alpha zeins) are deposited as an insoluble mass in the protein bodies of the endosperm. Because they are insoluble in water, most structural studies are performed in alcohol solutions. To solve the question raised by several authors about denaturation of the a zein structure by alcohol, we analyze the secondary structure of a zeins prepared with and without solubilization in alcohol (corn gluten meal and protein bodies with high concentrations of a zeins and traces of beta zeins). The secondary structures of a zeins are analyzed in the solid state by Fourier transform IR spectroscopy (FTIR) in KBr penets and solid-state 13C-NMR spectroscopy. The proportion of secondary structures obtained by FTIR of a zeins prepared with and without solubilization in alcohol yield almost identical proportions of a helices and (3 sheets. The proportion of a helices (43%) agrees with that measured by circular dichroism in an alcohol solution. However, the proportion of beta sheets (28%) is higher than the one measured by the same technique. Gluten and protein body samples with high beta zein content showed higher beta sheet and lower a helix proportions than that obtained for a zein preparations. The solid-state 13C-NMR spectra show the carbonyl peak for the a zeins at Delta 176 and for the sample rich in beta zeins at gama 172, which demonstrares the presence of a high C9ntent of a helices and beta sheets, respectively. These results indicare that alcohol solubilization does not affect the conformation of a zeins, validating the secondary structure measurements in solution.
653 $aalfa Zeins
653 $aConformação
653 $aEstado sólido
653 $aEstrutura Secundárias
653 $aNMR
653 $aSpectroscopia IR Fourier
700 1 $aBICUDO, T. C.
700 1 $aCOLNAGO, L. A.
773 $tBiopolymers (Biospectroscopy)$gv. 72, 421-426, 2003.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
31/05/2010 |
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Data da última atualização: |
14/06/2017 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
JUNQUEIRA, N. T. V.; FALEIRO, F. G.; BRAGA, M. F. |
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Afiliação: |
Nilton Tadeu Vilela Junqueira, CPAC; Fábio Gelape Faleiro, CPAC; Marcelo Fideles Braga, CPAC. |
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Título: |
BRS Ouro Vermelho: híbrido de maracujazeiro-azedo com maior quantidade de vitamina C. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados; Brasília, DF: Embrapa Transferência de Tecnologia, 2009. |
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Descrição Física: |
1 folder. |
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Idioma: |
Português |
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Notas: |
Equipe técnica: Nilton T. Vivela Junqueira; Fabio Gelape Faleiro; Marcelo Fideles Braga; José Ricardo Peixoto; Rogerio de Sá Borges; Soraya Carvalho Barrios de Araujo; José de Ribamar N. dos Anjos; Dalva Domenech Tupinambá; Solange R. Monteiro de Andrade; Ana Maria Barros; Adelise de Almeida Lima; Francisco Ferraz Laranjeira; Luiz Sebastião Poltronieri, CPATU; Marco Antônio S. Vasconcellos; Graciele Bellon; Keize Pereira Junqueira; Daniel Anacleto da Costa Lage; Erivanda Carvalho dos Santos; Carolina de Faria Vaz; Luciana Sobral de Souza; Dalvimar Gomes Pereira da Silva; Cristiane Andréa de Lima; Ciro Scaranari. |
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Thesagro: |
Maracujá; Passiflora Edulis; Produtividade; Variedade. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01215nam a2200193 a 4500
001 1854203
005 2017-06-14
008 2009 bl uuuu u0uu1 u #d
100 1 $aJUNQUEIRA, N. T. V.
245 $aBRS Ouro Vermelho$bhíbrido de maracujazeiro-azedo com maior quantidade de vitamina C.
260 $aPlanaltina, DF: Embrapa Cerrados; Brasília, DF: Embrapa Transferência de Tecnologia$c2009
300 $c1 folder.
500 $aEquipe técnica: Nilton T. Vivela Junqueira; Fabio Gelape Faleiro; Marcelo Fideles Braga; José Ricardo Peixoto; Rogerio de Sá Borges; Soraya Carvalho Barrios de Araujo; José de Ribamar N. dos Anjos; Dalva Domenech Tupinambá; Solange R. Monteiro de Andrade; Ana Maria Barros; Adelise de Almeida Lima; Francisco Ferraz Laranjeira; Luiz Sebastião Poltronieri, CPATU; Marco Antônio S. Vasconcellos; Graciele Bellon; Keize Pereira Junqueira; Daniel Anacleto da Costa Lage; Erivanda Carvalho dos Santos; Carolina de Faria Vaz; Luciana Sobral de Souza; Dalvimar Gomes Pereira da Silva; Cristiane Andréa de Lima; Ciro Scaranari.
650 $aMaracujá
650 $aPassiflora Edulis
650 $aProdutividade
650 $aVariedade
700 1 $aFALEIRO, F. G.
700 1 $aBRAGA, M. F.
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Embrapa Amazônia Oriental (CPATU) |
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