Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/04/2016 |
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Data da última atualização: |
10/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FALEIRO, F. G.; KANNAN, B.; ALTPETER, F. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC; BASKARAN KANNAN, UNIVERSITY OF FLORIDA; FREDY ALTPETER, UNIVERSITY OF FLORIDA. |
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Título: |
Regeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 124, n. 1, p. 57-67, January 2016. |
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DOI: |
10.1007/s11240-015-0874-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Elephantgrass (Pennisetum purpureum, 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for lignocellulosic biofuel production. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum, 2n = 2x = 14) may allow improvement of drought tolerance and biomass quality. These interspecific hybrids are male and female sterile due to their triploid genome (2n = 3x = 21). Chromosome doubling of the triploid hybrids may restore fertility, permitting a backcross with the recurrent or other elephantgrass parents to enhance biomass yield and persistence. In this study, chromosome doubling of productive interspecific hybrids was performed in vitro. Immature inflorescence cross-sections of five interspecific hybrids with good agronomic performance were used as explants for establishment of embryogenic tissue cultures. These calli were treated with different antimitotic agents, oryzalin or trifluralin. Chromosome doubling was achieved in all five interspecific hybrids and a total of 74 plants with altered ploidy were confirmed by flow cytometry. Stomatal size determination was a suitable screening tool for identification of hexaploid events. Genotypes MN18 and MN51 had the highest number of plants with altered ploidy of 29 and 27, respectively. Oryzalin at 5 µM was the most effective antimitotic treatment and produced 55 of the 74 plants with altered ploidy. The most vigorous lines were grown to maturity and produced viable pollen. MenosAbstract: Elephantgrass (Pennisetum purpureum, 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for lignocellulosic biofuel production. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum, 2n = 2x = 14) may allow improvement of drought tolerance and biomass quality. These interspecific hybrids are male and female sterile due to their triploid genome (2n = 3x = 21). Chromosome doubling of the triploid hybrids may restore fertility, permitting a backcross with the recurrent or other elephantgrass parents to enhance biomass yield and persistence. In this study, chromosome doubling of productive interspecific hybrids was performed in vitro. Immature inflorescence cross-sections of five interspecific hybrids with good agronomic performance were used as explants for establishment of embryogenic tissue cultures. These calli were treated with different antimitotic agents, oryzalin or trifluralin. Chromosome doubling was achieved in all five interspecific hybrids and a total of 74 plants with altered ploidy were confirmed by flow cytometry. Stomatal size determination was a suitable screening tool for identification of hexaploid events. Genotypes MN18 and MN51 had the highest number of plants with altered ploidy of 29 and 27, respectively. Oryzalin at 5 µM was the most effective antimitotic treatment and produced 55 of the 74 plants with altered ploidy. The most vigorous lines we... Mostrar Tudo |
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Palavras-Chave: |
Citometria de fluxo; Duplicação de cromossomo; Orizalina; Trifuralina. |
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Thesagro: |
Biocombustível; Capim elefante; Cromossoma; Cultura de tecido; Estômato; Matéria prima; Pennisetum Purpureum; Planta forrageira. |
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Thesaurus NAL: |
Biofuels; Chromosomes; Feedstocks; Forage; Stomata; Tissue culture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142227/1/Regeneration-of-fertile.pdf
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Marc: |
LEADER 02651naa a2200373 a 4500
001 2043082
005 2017-08-10
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1007/s11240-015-0874-4$2DOI
100 1 $aFALEIRO, F. G.
245 $aRegeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents.
260 $c2016
520 $aAbstract: Elephantgrass (Pennisetum purpureum, 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for lignocellulosic biofuel production. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum, 2n = 2x = 14) may allow improvement of drought tolerance and biomass quality. These interspecific hybrids are male and female sterile due to their triploid genome (2n = 3x = 21). Chromosome doubling of the triploid hybrids may restore fertility, permitting a backcross with the recurrent or other elephantgrass parents to enhance biomass yield and persistence. In this study, chromosome doubling of productive interspecific hybrids was performed in vitro. Immature inflorescence cross-sections of five interspecific hybrids with good agronomic performance were used as explants for establishment of embryogenic tissue cultures. These calli were treated with different antimitotic agents, oryzalin or trifluralin. Chromosome doubling was achieved in all five interspecific hybrids and a total of 74 plants with altered ploidy were confirmed by flow cytometry. Stomatal size determination was a suitable screening tool for identification of hexaploid events. Genotypes MN18 and MN51 had the highest number of plants with altered ploidy of 29 and 27, respectively. Oryzalin at 5 µM was the most effective antimitotic treatment and produced 55 of the 74 plants with altered ploidy. The most vigorous lines were grown to maturity and produced viable pollen.
650 $aBiofuels
650 $aChromosomes
650 $aFeedstocks
650 $aForage
650 $aStomata
650 $aTissue culture
650 $aBiocombustível
650 $aCapim elefante
650 $aCromossoma
650 $aCultura de tecido
650 $aEstômato
650 $aMatéria prima
650 $aPennisetum Purpureum
650 $aPlanta forrageira
653 $aCitometria de fluxo
653 $aDuplicação de cromossomo
653 $aOrizalina
653 $aTrifuralina
700 1 $aKANNAN, B.
700 1 $aALTPETER, F.
773 $tPlant Cell, Tissue and Organ Culture$gv. 124, n. 1, p. 57-67, January 2016.
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Registro original: |
Embrapa Cerrados (CPAC) |
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