Data/hora: 23/06/2024 10:09:28

Biblioteca(s): | Embrapa Agricultura Digital.
Data corrente: | 26/03/2009
Data da última atualização: | 15/01/2020
Autoria: | FALCAO, P. R. K.; TEIXEIRA, K. R. dos.
Afiliação: | PAULA REGINA KUSER FALCAO, CNPTIA; KATIA REGINA DOS SANTOS TEIXEIRA, CNPAB.
Título: | Evaluation of point mutation in genes related to ketogluconic acid production by Gluconacetobacter diazotrophicus.
Ano de publicação: | 2008
Fonte/Imprenta: | In: INTERNATIONAL CONFERENCE OF BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 4., 2008, Salvador. Proceedings... Salvador: AB³C, 2008.
Páginas: | Não paginado.
Idioma: | Inglês
Notas: | X-Meeting 2008.
Conteúdo: | Here, bioinformatic approach based on protein modeling was applied to two ORFs related to ketogluconates production, gluconate 5-dehydrogenase and 2-ketoglucone reductase. The ORFs were analyzed and identification of regions that play an important role in substrate binding/recognition presents an opportunity for stability prediction and design of mutagenesis strategy. Gluconate-5-dehydrogenase was modelled using as a 3D model the crystal structure of Gluconate 5-dehydrogenase from Thermotoga maritima (PDB ID: vl8) whereas crystal structure of a NDA-dependent p-glycerate dehydrogenase (PDB ID:1gdh) was used to model 2-ketogluconate reductase. The sequence identities for these proteins are 36% and 34%, respectively. Based on this in silico model and with the prediction performed with CUPSAT [4], it was proposed to construct the mutants His289Asn and Glu271Ser of 2-ketoglucone reductase.
Palavras-Chave: | Bioinformática; Gluconacetobacter diazotrophics; Oxidação da glicose; Produção de ácido.
Thesagro: | Fixação de Nitrogênio.
Thesaurus Nal: | Bioinformatics; genes.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Agricultura Digital (CNPTIA)

Exemplares:

CNPTIA - (12687-1) - UPC - RA - PP -  -  - 2009.00056 - DES
CNPTIA - (12687-2) - UPC - RA - DD -  -  -  - DIS