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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ALMEIDA, S. F. C. M.; FONSECA, J. F. da; BALARO, M. F. A.; ALMEIDA, J. G.; PINTO, P. H. N.; MOURA, A. B.; ROSA, R. M.; BRANDÃO, F. Z. |
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Afiliação: |
Universidade Federal Fluminense, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Use of two doses of cloprostenol at different intervals for estrus synchronization in Santa Inês ewes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 672, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 29th Annual Meeting of the Brazilian EmbryoTechnology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. |
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Conteúdo: |
Abstract: The objective of this study was to compare protocols for estrus synchronization, using two doses of cloprostenol in different intervals, in Martch, during the breeding season, in Santa Inês ewes, in the city of Cachoeiras de MacacuRJ. A total of 30 ewes (43.9±6.4 kg, 2.9±0.27 BCS and 3.4±1.6 years old) weaned at least for three months was equally allocated into three treatments, with intervals of: 11.5 days (G11.5: n=10), 9 days (G9: n=10) or 7 days (G7: n=10). The dose used for each administration was 37.5 µg cloprostenol (Estron®, Agener União, São Paulo, Brazil) intramuscularly. Transrectal ultrasound evaluations were performed (B-mode; SonoScape®, Shenzhen, China) for monitoring the follicular and luteal dynamic, daily from 5 d before the first dose, and every 12 h after both administrations, again for 5 d or until ovulation. For estrus detection, females were teased individually every 12 h after each cloprostenol administration for 5 d and for 5 min per animal. Normal quantitative variables were subjected to ANOVA followed by Tukey test (P<0.05). Data concerning the rate of ewes in estrus (%) were evaluated by Fisher's exact test (P <0.05). The percentage of animals in estrus after the first dose did not differ (P>0.05) among groups: G11.5 60% (6/10); G9 80% (8/10) and G7: 80% (8/10) as well as the duration of estrus (G11.5: 24.0±13.1 h; G9: 37.5±7.7h and G7: 28.5±11.0 h. After the second dose, estrus presentation rates and duration of estrus also did not differ (P>0.05) among groups, respectively: G11.5 90% (9/10) and 29.3±12.2 h; G9 ? 100% (10/10) and 36.0±10.4 h; and G7 80% (8/10) and 31.5±8.9 h. There was no statistical difference (P>0.05) in the intervals from the second dose to the onset of estrus, end of estrus, and from estrus to ovulation between G11.5 (48.5±8.9 h; 80.0±8.5 h; 35.0±20.1 h), G9 (50.3±13.1 h; 83.0±9.1 h; 25.5±12.2 h) and G7 (36.5±6.2 h; 68.0±14.0 h and 20.3±6.1 h). The interval from the administration of the second dose to ovulation differed (P<0.05) among groups G11.5 (78.7±9.4 h), G9 (75.5±8.3) h e G7 (56.8±6.2 h). The G7 anticipated ovulation and presented a lower standard deviation (P<0.05). In conclusion, it appears that animals in the G7 were close to follicular dominance period or were already with the dominant follicles while G11.5 and G9 might still be in the beginning of the follicular wave. The three protocols were effective for estrus synchronization in Santa Inês ewes. MenosAbstract: The objective of this study was to compare protocols for estrus synchronization, using two doses of cloprostenol in different intervals, in Martch, during the breeding season, in Santa Inês ewes, in the city of Cachoeiras de MacacuRJ. A total of 30 ewes (43.9±6.4 kg, 2.9±0.27 BCS and 3.4±1.6 years old) weaned at least for three months was equally allocated into three treatments, with intervals of: 11.5 days (G11.5: n=10), 9 days (G9: n=10) or 7 days (G7: n=10). The dose used for each administration was 37.5 µg cloprostenol (Estron®, Agener União, São Paulo, Brazil) intramuscularly. Transrectal ultrasound evaluations were performed (B-mode; SonoScape®, Shenzhen, China) for monitoring the follicular and luteal dynamic, daily from 5 d before the first dose, and every 12 h after both administrations, again for 5 d or until ovulation. For estrus detection, females were teased individually every 12 h after each cloprostenol administration for 5 d and for 5 min per animal. Normal quantitative variables were subjected to ANOVA followed by Tukey test (P<0.05). Data concerning the rate of ewes in estrus (%) were evaluated by Fisher's exact test (P <0.05). The percentage of animals in estrus after the first dose did not differ (P>0.05) among groups: G11.5 60% (6/10); G9 80% (8/10) and G7: 80% (8/10) as well as the duration of estrus (G11.5: 24.0±13.1 h; G9: 37.5±7.7h and G7: 28.5±11.0 h. After the second dose, estrus presentation rates and duration of estrus also did not diff... Mostrar Tudo |
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Palavras-Chave: |
Oestrus synchronization; Prostaglandin; Santa inês. |
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Thesagro: |
Ciclo estral; Ovelha; Ovino; Ovulação; Reprodução animal; Sincronização do cio. |
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Thesaurus Nal: |
Ewes; Ovulation; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162928/1/cnpc-2015-Use-of-two.pdf
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Marc: |
LEADER 03765nam a2200361 a 4500
001 2074299
005 2019-09-23
008 2015 bl uuuu u00u1 u #d
100 1 $aALMEIDA, S. F. C. M.
245 $aUse of two doses of cloprostenol at different intervals for estrus synchronization in Santa Inês ewes.$h[electronic resource]
260 $aAnimal Reproduction, v. 12, n. 3, p. 672, Jul./Sept. 2015.$c2015
500 $aProceedings of the 29th Annual Meeting of the Brazilian EmbryoTechnology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015.
520 $aAbstract: The objective of this study was to compare protocols for estrus synchronization, using two doses of cloprostenol in different intervals, in Martch, during the breeding season, in Santa Inês ewes, in the city of Cachoeiras de MacacuRJ. A total of 30 ewes (43.9±6.4 kg, 2.9±0.27 BCS and 3.4±1.6 years old) weaned at least for three months was equally allocated into three treatments, with intervals of: 11.5 days (G11.5: n=10), 9 days (G9: n=10) or 7 days (G7: n=10). The dose used for each administration was 37.5 µg cloprostenol (Estron®, Agener União, São Paulo, Brazil) intramuscularly. Transrectal ultrasound evaluations were performed (B-mode; SonoScape®, Shenzhen, China) for monitoring the follicular and luteal dynamic, daily from 5 d before the first dose, and every 12 h after both administrations, again for 5 d or until ovulation. For estrus detection, females were teased individually every 12 h after each cloprostenol administration for 5 d and for 5 min per animal. Normal quantitative variables were subjected to ANOVA followed by Tukey test (P<0.05). Data concerning the rate of ewes in estrus (%) were evaluated by Fisher's exact test (P <0.05). The percentage of animals in estrus after the first dose did not differ (P>0.05) among groups: G11.5 60% (6/10); G9 80% (8/10) and G7: 80% (8/10) as well as the duration of estrus (G11.5: 24.0±13.1 h; G9: 37.5±7.7h and G7: 28.5±11.0 h. After the second dose, estrus presentation rates and duration of estrus also did not differ (P>0.05) among groups, respectively: G11.5 90% (9/10) and 29.3±12.2 h; G9 ? 100% (10/10) and 36.0±10.4 h; and G7 80% (8/10) and 31.5±8.9 h. There was no statistical difference (P>0.05) in the intervals from the second dose to the onset of estrus, end of estrus, and from estrus to ovulation between G11.5 (48.5±8.9 h; 80.0±8.5 h; 35.0±20.1 h), G9 (50.3±13.1 h; 83.0±9.1 h; 25.5±12.2 h) and G7 (36.5±6.2 h; 68.0±14.0 h and 20.3±6.1 h). The interval from the administration of the second dose to ovulation differed (P<0.05) among groups G11.5 (78.7±9.4 h), G9 (75.5±8.3) h e G7 (56.8±6.2 h). The G7 anticipated ovulation and presented a lower standard deviation (P<0.05). In conclusion, it appears that animals in the G7 were close to follicular dominance period or were already with the dominant follicles while G11.5 and G9 might still be in the beginning of the follicular wave. The three protocols were effective for estrus synchronization in Santa Inês ewes.
650 $aEwes
650 $aOvulation
650 $aReproduction
650 $aSheep
650 $aCiclo estral
650 $aOvelha
650 $aOvino
650 $aOvulação
650 $aReprodução animal
650 $aSincronização do cio
653 $aOestrus synchronization
653 $aProstaglandin
653 $aSanta inês
700 1 $aFONSECA, J. F. da
700 1 $aBALARO, M. F. A.
700 1 $aALMEIDA, J. G.
700 1 $aPINTO, P. H. N.
700 1 $aMOURA, A. B.
700 1 $aROSA, R. M.
700 1 $aBRANDÃO, F. Z.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
27/08/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
Vítor da Silveira Falavigna; Diogo Denardi Porto, CNPUV; Vanessa Buffon, CNPUV; Márcia Margis-Pinheiro; Giancarlo Pasquali; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Differential transcriptional profiles of dormancy-related genes in apple buds |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 32, p. 796-813, 2014. |
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Idioma: |
Inglês |
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Notas: |
DOI 10.1007/s11105-013-0690-0 |
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Conteúdo: |
The production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees. MenosThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts codin... Mostrar Tudo |
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Palavras-Chave: |
Apple; Castel Gala; Fuji Standard; Royal Gala. |
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Thesagro: |
Dormencia; Genetica vegetal; Maçã; Temperatura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107373/1/Falavigna2014-Genes-Apple-Buds.pdf
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Marc: |
LEADER 02604naa a2200289 a 4500
001 1993551
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aFALAVIGNA, V. da S.
245 $aDifferential transcriptional profiles of dormancy-related genes in apple buds$h[electronic resource]
260 $c2014
500 $aDOI 10.1007/s11105-013-0690-0
520 $aThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees.
650 $aDormencia
650 $aGenetica vegetal
650 $aMaçã
650 $aTemperatura
653 $aApple
653 $aCastel Gala
653 $aFuji Standard
653 $aRoyal Gala
700 1 $aPORTO, D. D.
700 1 $aBUFFON, V.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Molecular Biology Reporter$gv. 32, p. 796-813, 2014.
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Embrapa Uva e Vinho (CNPUV) |
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