Data/hora: 13/06/2024 10:57:02

Biblioteca(s): | Embrapa Uva e Vinho.
Data corrente: | 27/06/2017
Data da última atualização: | 06/05/2019
Autoria: | SILVA, J. M. F; RWAHNIH, M. A.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M.
Afiliação: | João Marcos Fagundes Silva, University of Bras?lia, Bras?lia, DF, Brazil; Maher Al Rwahnih, Department of Plant Pathology, University of California, Davis, CA, USA; Rosana Blawid, University of Bras? ? lia, Bras? ? lia, DF, Brazil; Tatsuya Nagata, University of Bras?lia, Bras?lia, DF, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV.
Título: | Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1.
Ano de publicação: | 2017
Fonte/Imprenta: | Virus Genes, v.53, n. 4, p. 667-671 [online], 2017.
DOI: | 10.1007/s11262-017-1470-y
Idioma: | Inglês
Conteúdo: | In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1
Palavras-Chave: | GEV-1; Grapevine enamovirus-1; High-throughput sequencing.
Thesaurus Nal: | Luteoviridae.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/161127/1/GEV1-Virus-Genes-Silva-et-al-published-June-03-2017.pdf
Registro original: | Embrapa Uva e Vinho (CNPUV)
Exemplares: | CNPUV - (17286-1) - UPC - AP - DD - 634.8 -  -  - DIS