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| Registros recuperados : 135 | |
| 81. |  | POLETTO, C. M.; MAGALHÃES, L. C. D. R.; CUNHA, J. R.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; SALUM, T. F. C. T46 new fungal lipases for biodiesel production. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 38., 2016, Baltimore, MD. [Abstracts ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2016.| Biblioteca(s): Embrapa Agroenergia. |
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| 82. | | SHIMIZU, A. M. F.; JARAMILLO, P. M. D.; MIDORIKAWA, G. E. O.; SILVA, A. S.; BON, E. P. S.; FAVARO, L. C. de L. Transformação mediada por Agrobacterium tumefaciens do fungo celulótico Trichoderma harzianum CFAM422 e obtenção de uma biblioteca de transformantes. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 45-47| Biblioteca(s): Embrapa Agroenergia. |
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| 83. | | TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; JARAMILLO, P. M. D.; CUNHA, D. C. da; NUNES, J. F. R.; FAVARO, L. C. de L.; NORONHA, E. F. Transformação de Trichoderma harzianum BRM27158 por Agrobacterium tumefaciens e caracterização de uma biblioteca de transformantes. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 43.| Biblioteca(s): Embrapa Agroenergia. |
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| 84. | | NUNES, L. S.; FREIRE, Í. A.; RODRIGUES, K. B.; MIDORIKAWA, G. E. O.; QUECINE, M. C.; FAVARO, L. C. de L. In vitro assessment of inorganic phosphate-solubilizing potential of Bacillus, Paenibacillus, Peribacillus, and Alkalihalobacillus strains. In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. Resumo.| Biblioteca(s): Embrapa Agroenergia. |
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| 85. | | SANTOS, M. U. de O.; NUNES, L. S.; CARVALHO, B. A. R.; GUIMARÃES, J. P. S. M.; TEIXEIRA, B. R.; FAVARO, L. C. de L. Viability and conservation of a collection of filamentous fungi associated with sugarcane plants (Saccharum complex). In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. Resumo.| Biblioteca(s): Embrapa Agroenergia. |
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| 86. | | FERNANDES, L. M.; MEDEIROS, A. L. R. G. de; RIBEIRO, L. B. D. de A.; SANTOS, I. M. G.; ALVES, L. M.; FAVARO, L. C. de L. Viability and conservation of a collection of soybean-associated bacteria after preservation for more than 20 years by deep-freezing in glycerol. In: INTERNATIONAL SYMPOSIUM OF MICROBIOLOGY AND BIOTECHNOLOGY, 6., 2023, Viçosa, MG. Biotecnologia Microbiana Soluções para os grandes desafios globais: anais. Viçosa, MG: Universidade Federal de Viçosa, 2023. Resumo.| Biblioteca(s): Embrapa Agroenergia. |
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| 87. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 88. | | RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALUU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPOSITO, M. B.; ARAUJO, W. L. de; PIZZIRANI-KLEINER, A. A. Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Fungal Biology, v. 117, n. 7-8, p. 463-576, 2013.| Biblioteca(s): Embrapa Agroenergia. |
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| 89. | | FAVARO, L. C. de L.; MELO, I. S.; QUIRINO, B. F.; OLIVEIRA, A. C. B.; MENDES, T. D.; SALUM, T. F. C.; RODRIGUES, D. de S.; SOUZA, G. P.; FRANCO, P. F.; QUECINE, M. C. Brazilian macrobial diversity as a source of new strains for deconstruction of lignocellulosic biomass. Hechos Microbiológicos, v. 5, n. 2, suplemento 2, 2014. Trabalho apresentado no XXII Congresso Latinoamericano de Microbiología - ALAM 2014 e 4 Congresso Colombiano de Microbiología - 4 CCM 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 90. | | FAVARO, L. C. de L.; MELO, I. S. de; QUIRINO, B. F.; OLIVEIRA, A. C. B.; MENDES, T. D.; SALUM, T. F. C.; RODRIGUES, D. S.; SOUZA, G. P.; FRANCO, P. F.; QUECINE, M. C. Brazilian microbial diversity as a source of new strains for deconstruction of lignocellulosic biomass. Hechos Microbiológicos, v. 5, n. 2, p. 133, 2014. Suplemento. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014. Ref. TLP-310.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 91. | | SILVA, E. A. da; PACHECO, T. F.; MENDES, T. D.; LEMOS, M.; CARVALHO, F. B. de P.; FAVARO, L. C. de L.; MENDONCA, S.; SIQUEIRA, F. G. de; SOUZA JUNIOR, M. T. Combinação de extratos enzimáticos de basidiomicetos e ascomicetos para hidrólise do cacho vazio de dendê. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 88-94.| Biblioteca(s): Embrapa Agroenergia. |
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| 92. | | SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. Analytical Methods, n. 2, 2020.| Biblioteca(s): Embrapa Agroenergia. |
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| 93. | | SANTANA, M. W. P. R. de; RODRIGUES, K. B.; SANTOS, F. P. dos; MENDES, T. D.; DAMASO, M. C. T.; FAVARO, L. C. de L.; SIQUEIRA, F. G. de. Clonagem e expressão de genes de monoxigenases de polissacarídeos líticas (LPMOs) de fungos em Pichia pastoris. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 36-47.| Biblioteca(s): Embrapa Agroenergia. |
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| 94. | | SILVA, I. N. da; IKEDA, F. S.; FUJIMORI, I. S. T.; IEKA, L. F.; BAUERMANN, G. S.; CONTESINI, M. E.; BASILIO, E. R.; FAVARO, L.; SILVA, J. C. F. da. Controle químico em pós-emergência de vassourinha-de-botão perenizada em cultivo de milho. In: JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 12., 2023. Sinop. Resumos... Brasília, DF: Embrapa, 2023. p. 42. (Embrapa Agrossilvipastoril. Eventos Técnicos & Científicos, 1)| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 95. | | SANTIAGO, T. R.; PEREIRA, V. M.; SOUZA, W. R.; STEINDORFF, A. S.; DIAS, B. B. A.; GASPAR, M.; FAVARO, L. C. de L.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Genome-wide identification, characterization and expression profile analysis of expansins gene family in sugarcane (Saccharum spp.). PLoS One, v. 13, n. 1, artigo e0191081, 2018.| Biblioteca(s): Embrapa Agroenergia. |
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| 96. | | CARDOSO, S. L.; SOUZA, P. M.; RODRIGUES, K.; MOTA, I. de S.; FERREIRA FILHO, E.; FAVARO, L. C. de L.; ARAUJO, F. S.; HOMEM-DE-MELLO, M.; PESSOA, A.; SILVEIRA, D.; BAZZO, Y. M. F.; MAGALHÃES, P. O. L-Asparaginase type II from Fusarium proliferatum: heterologous expression and in silico analysis. Pharmaceutics, v. 15, 2352, 2023.| Biblioteca(s): Embrapa Agroenergia. |
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| 97. | | BARBOSA, M. F.; BERGMANN, J. C.; CARVALHO JUNIOR, A. R. de; SOUTO, B. de M.; OLIVEIRA, L. C. A. de; DUARTE, A. de O.; SALES, R. M. M.; FAVARO, L. C. de L.; RODRIGUES, C. M.; QUIRINO, B. F. Isolamento e identificação de microrganismos degradadores de lignina do bioma Cerrado. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 7., 2023, Brasília, DF. Anais... Brasília, DF: Embrapa, 2023. p. 18.| Biblioteca(s): Embrapa Agroenergia. |
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| 98. | | TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L. Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. Não paginado. SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019.| Biblioteca(s): Embrapa Agroenergia; Embrapa Agroindústria de Alimentos. |
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| 99. | | PEIXOTO, J. S. G.; JARAMILLO, P. M. D.; PEREIRA, V. M.; TAMIETTI, M. S.; AZEVEDO, J. L.; PEREIRA, J. O.; FILHO, S. A.; MELO, I. S.; FAVARO, L. C. de L.; MACHADO, F. Endophytic fungi of Paullinia cupana and Rhizophora mangle and their potential for lignocellulolytic enzymes production. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracajú, SE. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 100. | | TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L. Disruption of the glucose repressor gene cre1 in Trichoderma harzianum CFAM-422 and its effect on plant cell wall degrading enzymes production. In: FUNGAL GENETICS CONFERENCE, 29., 2017, Pacific Grove. Abstract book ... Bethesda: Genetics Society of America, 2017. p. 107.| Biblioteca(s): Embrapa Agroenergia; Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 135 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/03/2020 |
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Data da última atualização: |
27/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. |
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Afiliação: |
Caio de Oliveira Gorgulho Silva; Tallyta Santos Teixeira, Universidade Federal de Tocantins; Kelly Barreto Rodrigues; Amanda Araujo Souza; Antonielle Vieira Monclaro; THAIS DEMARCHI MENDES, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Analytical Methods, n. 2, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. MenosLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type... Mostrar Tudo |
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Palavras-Chave: |
Caracterização de LPMOs. |
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Categoria do assunto: |
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Marc: |
LEADER 02626naa a2200241 a 4500
001 2121326
005 2020-04-27
008 2020 bl uuuu u00u1 u #d
100 1 $aSILVA, C. de O. G.
245 $aCombination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity.$h[electronic resource]
260 $c2020
520 $aLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products.
653 $aCaracterização de LPMOs
700 1 $aTEIXEIRA, S. T.
700 1 $aRODRIGUES, K. B.
700 1 $aSOUZA, A. A.
700 1 $aMONCLARO, A. V.
700 1 $aMENDES, T. D.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aFAVARO, L. C. de L.
700 1 $aABDELNUR, P. V.
773 $tAnalytical Methods$gn. 2, 2020.
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