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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
11/10/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A.; JESUS, C. C.; CALEGARIO, R. F.; KITAJIMA, E. W.; NOVELLI, V. M.; ASTUA, J. de F. |
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Afiliação: |
GABRIELLA DIAS ARENA; PEDRO LUIS RAMOS-GONZÁLEZ; MARIA ANDRÉIA NUNES; CAMILA CHABI JESUS; RENATA FAIER CALEGARIO; ELLIOT WATANABE KITAJIMA; VALDENICE MOREIRA NOVELLI; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientia Agricola, v.74, n.1, p.1-10X, 2017 |
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ISSN: |
1678-992X |
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DOI: |
10.1590/1678-992X-2015-0380 |
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Idioma: |
Inglês |
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Conteúdo: |
Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum violaefolium ringspot virus, Coffee ringspot virus, Dichorhavirus MenosBrevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum ... Mostrar Tudo |
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Thesagro: |
Doença de planta; Virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02287naa a2200253 a 4500
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022 $a1678-992X
024 7 $a10.1590/1678-992X-2015-0380$2DOI
100 1 $aARENA, G. D.
245 $aArabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses.$h[electronic resource]
260 $c2017
520 $aBrevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum violaefolium ringspot virus, Coffee ringspot virus, Dichorhavirus
650 $aDoença de planta
650 $aVirus
700 1 $aRAMOS-GONZÁLEZ, P. L.
700 1 $aNUNES, M. A.
700 1 $aJESUS, C. C.
700 1 $aCALEGARIO, R. F.
700 1 $aKITAJIMA, E. W.
700 1 $aNOVELLI, V. M.
700 1 $aASTUA, J. de F.
773 $tScientia Agricola$gv.74, n.1, p.1-10X, 2017
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
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URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
13/01/2009 |
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Data da última atualização: |
10/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FIRMINO, A. A. P.; EVARISTO, R. G. S.; FRANCO, O. L.; CARNEIRO, R. M. D. G.; SILVEIRA, E. R.; SOUZA, D. S. L.; SILVA, L. P.; MAGALHÃES, B. S.; SILVA, M. C. M.; SOUSA, B. A.; SA, M. F. G. de; ROCHA, T. L. |
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Afiliação: |
ALEXANDRE AUGUSTO PEREIRA FIRMINO; RAPHAEL GARCIA DE SOUSA EVARISTO,; UCB; REGINA MARIA DECHECHI GOMES CARNEIRO, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; THALES LIMA ROCHA, CENARGEN. |
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Título: |
Identification of secondary metabolites from Canavalia ensiformis: perspectives for the use of metabolic engineering to control nematodes. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. |
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Páginas: |
p. 281. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Canavalia ensifomis. |
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Thesagro: |
Nematóide. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00868nam a2200265 a 4500
001 1190416
005 2024-05-10
008 2008 bl uuuu u01u1 u #d
100 1 $aFIRMINO, A. A. P.
245 $aIdentification of secondary metabolites from Canavalia ensiformis$bperspectives for the use of metabolic engineering to control nematodes.
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG$c2008
300 $ap. 281.$c1 CD-ROM.
650 $aNematóide
653 $aCanavalia ensifomis
700 1 $aEVARISTO, R. G. S.
700 1 $aFRANCO, O. L.
700 1 $aCARNEIRO, R. M. D. G.
700 1 $aSILVEIRA, E. R.
700 1 $aSOUZA, D. S. L.
700 1 $aSILVA, L. P.
700 1 $aMAGALHÃES, B. S.
700 1 $aSILVA, M. C. M.
700 1 $aSOUSA, B. A.
700 1 $aSA, M. F. G. de
700 1 $aROCHA, T. L.
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