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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
25/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, A. C. F.; MONERÓ, T. de O.; FRIGHETTO, R. T. S.; ALMEIDA, E. A. de. |
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Afiliação: |
ALINE CRISTINA FERREIRA RODRIGUES, IBILCE-UNESP; TATIANA DE OLIVEIRA MONERÓ, IBILCE-UNESP; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; EDUARDO ALVES DE ALMEIDA, IBILCE-UNESP. |
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Título: |
E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Environmental Science & Pollution Research, Berlin, v. 22, n. 22, p. 17367-17374, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia. MenosIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolate... Mostrar Tudo |
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Palavras-Chave: |
17b-Estradiol; Biotransformation enzymes; Hidrocarboneto aromático policíclico. |
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Thesagro: |
Oreochromis niloticus; Poluição da água; Substância tóxica; Tilápia nilótica. |
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Thesaurus Nal: |
Benzopyrenes; Polycyclic aromatic hydrocarbons; Water pollution. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02768naa a2200277 a 4500
001 2032529
005 2016-01-25
008 2015 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, A. C. F.
245 $aE2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations.$h[electronic resource]
260 $c2015
520 $aIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia.
650 $aBenzopyrenes
650 $aPolycyclic aromatic hydrocarbons
650 $aWater pollution
650 $aOreochromis niloticus
650 $aPoluição da água
650 $aSubstância tóxica
650 $aTilápia nilótica
653 $a17b-Estradiol
653 $aBiotransformation enzymes
653 $aHidrocarboneto aromático policíclico
700 1 $aMONERÓ, T. de O.
700 1 $aFRIGHETTO, R. T. S.
700 1 $aALMEIDA, E. A. de
773 $tEnvironmental Science & Pollution Research, Berlin$gv. 22, n. 22, p. 17367-17374, 2015.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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Cutter |
Registro |
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Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
10/03/1995 |
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Data da última atualização: |
15/02/2016 |
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Autoria: |
EUCLIDES, V. P. B.; EUCLIDES FILHO, K.; ARRUDA, Z. J. de; FIGUEIREDO, G. R. de. |
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Afiliação: |
EMBRAPA. Centro Nacional de Pesquisa de Gado de Corte (Campo Grande, MS). |
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Título: |
Suplementacao a pasto: uma alternativa para a produção de novilho precoce. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Campo Grande, MS: EMBRAPA, CNPGC, 1995. |
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Páginas: |
3 p. |
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Série: |
(EMBRAPA-CNPGC. CNPGC Divulga, 1). |
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Idioma: |
Português |
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Notas: |
CNPGC. Serie nao convencional. |
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Palavras-Chave: |
Pasto; Precoce; Suplementação. |
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Thesagro: |
Bovino; Brachiaria Decumbens; Novilho; Nutrição. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139014/1/cnpgc-divulga-01.pdf
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Marc: |
LEADER 00763naa a2200265 a 4500
001 1318253
005 2016-02-15
008 1995 bl uuuu u00u1 u #d
100 1 $aEUCLIDES, V. P. B.
245 $aSuplementacao a pasto$buma alternativa para a produção de novilho precoce.$h[electronic resource]
260 $c1995
300 $a3 p.
490 $a(EMBRAPA-CNPGC. CNPGC Divulga, 1).
500 $aCNPGC. Serie nao convencional.
650 $aBovino
650 $aBrachiaria Decumbens
650 $aNovilho
650 $aNutrição
653 $aPasto
653 $aPrecoce
653 $aSuplementação
700 1 $aEUCLIDES FILHO, K.
700 1 $aARRUDA, Z. J. de
700 1 $aFIGUEIREDO, G. R. de
773 $tCampo Grande, MS: EMBRAPA, CNPGC, 1995.
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