Data/hora: 23/06/2024 01:19:05

Biblioteca(s): 	Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	B - 1	
Data corrente: 	09/09/2021	
Data da última atualização: 	10/03/2023	
Autoria: 	ARRAIS, A. M.; MELLO, M. R. B. de; VERGANI, G. B.; FIGUEIRA, L. M.; ESTEVES, S. N.; PEREIRA, V. S. do A.; BARTLEWSKI, P. M.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da.	
Afiliação: 	ALINE MATOS ARRAIS, Universidade Federal Rural do Rio de Janeiro (UFRRJ); MARCO ROBERTO BOURG DE MELLO, Universidade Federal Rural do Rio de Janeiro (UFRRJ); GABRIEL BRUN VERGANI, Universidade Estadual Paulista (UNESP); LUCAS MACHADO FIGUEIRA; SERGIO NOVITA ESTEVES, CPPSE; VERONICA SCHINAIDER DO A PEREIRA, CPPSE; PAWEL MIECZYSLAW BARTLEWSKI; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista (UNESP); JOANNA MARIA GONÇALVES SOUZA-FABJAN; JEFERSON FERREIRA DA FONSECA, CNPC.	
Título: 	Nonsurgical embryo recovery from estrus-synchronized or superovulated Morada Nova ewes: a feasible strategy for sheep embryo banking.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Biopreservation and Biobanking, v. 19, n. 5, oct. 2021.	
DOI: 	10.1089/bio.2020.0125	
Idioma: 	Inglês	
Conteúdo: 	Abstract: This study assessed the feasibility of in vivo embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; n = 12), nine (G9; n = 12), or 12 (G12; n = 12) days. Half of the ewes in each group remained estrus synchronized only ((SYNCH)) and the other half was superovulated ((SOV)) with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences (p > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 +/- 0.9), G9 (6.2 +/- 0.7), and G12 (5.5 +/- 0.6). Estrus responses and NSER success rates did not vary (p > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later (p < 0.01) in G9(SYNCH) ewes compared with G6(SYNCH) and G12(SYNCH,) and the duration of estrus was similar to 19 hours greater (p < 0.01) in G9(SOV) than in G6(SOV). The average duration of the NSER procedure was 32.6 +/- 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (p > 0.05) for G6 (1.0 +/- 0.3 and 2.5 +/- 1.5), G9 (1.3 +/- 0.5 and 4.8 +/- 2.0), and G12 groups (1.0 +/- 0.3 and 4.8 +/- 2.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs	
Palavras-Chave: 	In vivo embryo production; Naturalized breed; NSER; Programa de conservação; Raça Morada Nova; Reproductive biotechnologies.	
Thesagro: 	Ovino; Recurso Genético; Reprodução Animal; Sincronização do Cio; Superovulação.	
Thesaurus Nal: 	Animal reproduction; Conservation programs; Estrus synchronization; Ewes; Sheep; Small ruminants; Superovulation.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CNPC - (39878-1) - UPC - AP - DD -  -  -  - DIS
CPPSE - (25502-1) - UPC - AP - DD - PROCI-2021.00200 - ARR - 2021.00225 - DIS