Data/hora: 19/06/2024 15:45:23

Biblioteca(s): | Embrapa Solos.
Data corrente: | 02/05/2022
Data da última atualização: | 03/05/2022
Autoria: | ESPIRITO SANTO, M. C. do; THEMA, F. T.; PELLEGRINI, V. de O. A.; KANE, A. O.; GUIMARAES, F. E. G.; FILGUEIRAS, J. G.; NOVOTNY, E. H.; AZEVEDO, E. R. de; POLIKARPOV, I.
Afiliação: | MELISSA CRISTINA DO ESPIRITO SANTO, USP; FORCE TEFO THEMA, BOTSWANA UNIVERSITY OF AGRICULTURE AND NATURAL RESOURCES; VANESSA DE OLIVEIRA ARNOLDI PELLEGRINI, USP; AISSATA OUSMANE KANE, USP; FRANCISCO EDUARDO GONTIJO GUIMARAES, USP; JEFFERSON G. FILGUEIRAS, UFF; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO RIBEIRO DE AZEVEDO, USP; IGOR POLIKARPOV, USP.
Título: | When the order matters: impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse.
Ano de publicação: | 2022
Fonte/Imprenta: | Industrial Crops and Products, v. 180, 114708, Jun. 2022.
DOI: | https://doi.org/10.1016/j.indcrop.2022.114708
Idioma: | Inglês
Conteúdo: | Dilute acid and alkaline pretreatments are commonly used to fractionate lignocellulosic biomass for its conversion into biofuels and renewable materials. In the current work, we compare the structure and morphology of one and two-step alkaline and acid pretreated sugarcane bagasse (SCB) and differences in their enzymatic hydrolysis rates and yields. We used physical techniques such as nuclear magnetic resonance (NMR) and X-ray diffraction to shed light on physical and morphological changes introduced by the pretreatments in SCB. We also applied solid state NMR procedures which allowed us to separate xylan structural conformations inside pretreated plant biomass samples. Our results reveal that practically all xylan in two-step "alkaline first" (NaOH 1% + H2SO4 1%) pretreated samples adopts two-fold ribbon-like conformation, tightly associated with crystalline cellulose. On the contrary, a main part of xylan in the SCB samples after "acid first" (H2SO4 1% + NaOH 1%) combined pretreatment has a three-fold screw conformation characteristic of xylan bound to lignin. Modifications in the biomass composition, physical structure and xylan conformation introduced by the alternative pretreatments contribute to the observed differences in the enzymatic hydrolysis rates and yields. These results could be relevant for analysis and optimization of pretreatments and enzymatic hydrolysis of lignocellulosic biomass in general.
Palavras-Chave: | Estrutura física; Hidrólise enzimática; Physical structure; Xilano.
Thesagro: | Bagaço; Biomassa; Cana de Açúcar.
Thesaurus Nal: | Biomass; Enzymatic hydrolysis; Sugarcane bagasse; Xylan.
Categoria do assunto: | P Recursos Naturais, Ciências Ambientais e da Terra
Registro original: | Embrapa Solos (CNPS)
Exemplares: | CNPS - (21057-1) - UPC - AP - DD -  -  -  - DIS