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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/03/2006 |
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Data da última atualização: |
26/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GERMANO, M. G.; MENNA, P.; MOSTASSO, F. L.; HUNGRIA, M. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Reading, v. 56, n. 1, p. 217-229, Jan. 2006. |
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DOI: |
10.1099/ijs.0.02917-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined. MenosGenetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts... Mostrar Tudo |
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Thesagro: |
Fixação de Nitrogênio. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03220naa a2200181 a 4500
001 1468978
005 2024-07-26
008 2006 bl uuuu u00u1 u #d
024 7 $a10.1099/ijs.0.02917-0$2DOI
100 1 $aGERMANO, M. G.
245 $aRFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species.$h[electronic resource]
260 $c2006
520 $aGenetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined.
650 $aFixação de Nitrogênio
700 1 $aMENNA, P.
700 1 $aMOSTASSO, F. L.
700 1 $aHUNGRIA, M.
773 $tInternational Journal of Systematic and Evolutionary Microbiology, Reading$gv. 56, n. 1, p. 217-229, Jan. 2006.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 8 | |
| 1. |  | LIMA, M. de M.; LEMOS, W. de P.; ESPÍRITO SANTO, L. N. do; SOARES, C. S. Diversidade de formigas (Hym., Formicidae) em dois sistemas distintos de cultivos de dendê Elaeis guineensis no Nordeste Paraense. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA, 14., 2010, Belém, PA. Bolsista de iniciação científica: um aporte ao desenvolvimento da pesquisa agropecuária: anais. Belém, PA: Embrapa Amazônia Oriental, 2010. 1 CD-ROM. PIBIC 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | LEMOS, W. de P.; LIMA, M. de M.; ESPÍRITO SANTO, L. N. do; BISPO, C. J. C. Artropodofauna de solo com potencial de uso em programas de controle biológico de pragas em cultivos alternativos de palma de óleo (Elaeis Guineensis). In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD-ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | LIMA, M. de M.; LEMOS, W. de P.; SOARES, A. C. S.; ESPÍRITO SANTO, L. N. do. Atividade inseticida de extratos de nim (Azadirachta indica) sobre imaturos de Tenebrio molitor (Col., Curculionadae) em laboratório. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA, 14., 2010, Belém, PA. Bolsista de iniciação científica: um aporte ao desenvolvimento da pesquisa agropecuária: anais. Belém, PA: Embrapa Amazônia Oriental, 2010. 1 CD-ROM. PIBIC 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | LIMA, M. de M.; LEMOS, W. de P.; ESPÍRITO SANTO, L. N. do; BISPO, C. J. C. De que forma diferentes sistemas de cultivo de palma de óleo e a precipitação afetam a diversidade de morfoespécies de aranhas? In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | LIMA, M. de M.; LEMOS, W. de P.; ESPÍRITO SANTO, L. N. do; BISPO, C. J. C. Influência climática sobre a diversidade de inimigos naturais coletados com armadilhas adesivas em diferentes sistemas de cultivo de palma de óleo (elaeis guineensis) no município de Tomé-Açu, Pará. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD-ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | LEMOS, W. de P.; ESPÍRITO SANTO, L. N. do; LIMA, M. de M.; OLIVEIRA, T. A. de. Inimigos naturais de solo em sistemas de cultivo de palma de óleo (Elaeis guineensis) no município de Tomé-Açu, PA. Cadernos de Agroecologia, v. 10, n. 3, out. 2015. Edição dos Resumos do IX Congresso Brasileiro de Agroecologia e IV Seminário Estadual de Agroecologia, Belém, PA, set./out. 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | ESPÍRITO SANTO, L. N.; LEMOS, W. de P.; BONALDO, A. B.; LO-MAN-HUNG, N. F.; BISPO, C. J. C.; CASTELLANI, D. C.; LIMA, M. de M. Diversidade de aranhas de solo (Arachnida: araneae) em sistema alternativo de cultivo de dendê Elaeis guineensis no Pará, Brasil. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | ESPIRITO SANTO, L. N. do; LEMOS, W. de P.; BISPO, C. J. C.; CASTELLANI, D. C.; SOUZA, E. P.; LIMA, M. de M. Inimigos naturais associados a dendezeiro Elaeis guineensis cultivado em sistema agroflorestal no Nordeste paraense. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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