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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/10/1995 |
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Data da última atualização: |
05/10/1995 |
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Autoria: |
CASTRIC, P. A.; CONN, E. E. |
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Afiliação: |
Department of Biochemistry and Biophysics, University of California, Davis, California. |
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Título: |
Formation of b-cyanoalanine by o-acetylserine sulfhydrylase. |
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Ano de publicação: |
1971 |
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Fonte/Imprenta: |
Journal of Bacteriology, v.108, n.1, p.132-136, 1971. |
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Idioma: |
Inglês |
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Conteúdo: |
Cell-free extracts of bacillus megaterium form B-cyanolanine (B-CNA)-14C from Na14CN and L-cysteine, O-acetyl-serine or, to a lesser extent, L-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of B-CNA from cysteine and cyanide. The formation of B-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or L-djenkolic acid as souces: such cells also exhibit an increased ability to from cysteine when compared with cells grown on cysteine as the sulfur source. B-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification on the O-acetylsering sulfhydrylase resulted in the co-purification of the B-CNA- forming activity. |
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Palavras-Chave: |
HCN; Purificacao de enzinas. |
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Thesagro: |
Purificação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01251naa a2200169 a 4500
001 1647309
005 1995-10-05
008 1971 bl --- 0-- u #d
100 1 $aCASTRIC, P. A.
245 $aFormation of b-cyanoalanine by o-acetylserine sulfhydrylase.
260 $c1971
520 $aCell-free extracts of bacillus megaterium form B-cyanolanine (B-CNA)-14C from Na14CN and L-cysteine, O-acetyl-serine or, to a lesser extent, L-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of B-CNA from cysteine and cyanide. The formation of B-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or L-djenkolic acid as souces: such cells also exhibit an increased ability to from cysteine when compared with cells grown on cysteine as the sulfur source. B-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification on the O-acetylsering sulfhydrylase resulted in the co-purification of the B-CNA- forming activity.
650 $aPurificação
653 $aHCN
653 $aPurificacao de enzinas
700 1 $aCONN, E. E.
773 $tJournal of Bacteriology$gv.108, n.1, p.132-136, 1971.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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