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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
28/04/2021 |
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Data da última atualização: |
28/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CORNACINI, M. R.; MANOEL, R. O.; ALCANTARA, M. A. M.; MORAES, M. L. T.; SILVA, E. A. A.; PEREIRA NETO, L. G.; SEBBENN, A. M.; ROSSINI, B. C.; MARINO, C. L. |
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Afiliação: |
MAIARA R. CORNACINI, UNESP, Botucatu-SP; RICARDO O. MANOEL, UNESP, Botucatu-SP; MARCELO A. M. ALCANTARA, UNESP, Botucatu-SP; MARIO L. T. MORAES, UNESP, Ilha Solteira-SP; EDVALDO A. A. SILVA, UNESP, Botucatu-SP; LEONEL GONCALVES PEREIRA NETO, CNPASA; ALEXANDRE M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO, Piracicaba-SP; BRUNO C. ROSSINI, UNESP, Botucatu-SP; CELSO L. MARINO, UNESP, Botucatu-SP. |
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Título: |
Detection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Molecular Biology Reports, 2021. |
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ISSN: |
0301-4851 |
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DOI: |
https://doi.org/10.1007/s11033-021-06338-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Astronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. |
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Palavras-Chave: |
Conservation genetics; Microsatellite markers. |
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Thesagro: |
Anacardiaceae; Astronium Fraxinifolium. |
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Thesaurus Nal: |
Conservation plants; Genetic markers; Microsatellite repeats; Population genetics. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222904/1/mbr-2021.pdf
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Marc: |
LEADER 02452naa a2200337 a 4500
001 2131540
005 2021-04-28
008 2021 bl uuuu u00u1 u #d
022 $a0301-4851
024 7 $ahttps://doi.org/10.1007/s11033-021-06338-5$2DOI
100 1 $aCORNACINI, M. R.
245 $aDetection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species.$h[electronic resource]
260 $c2021
520 $aAstronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management.
650 $aConservation plants
650 $aGenetic markers
650 $aMicrosatellite repeats
650 $aPopulation genetics
650 $aAnacardiaceae
650 $aAstronium Fraxinifolium
653 $aConservation genetics
653 $aMicrosatellite markers
700 1 $aMANOEL, R. O.
700 1 $aALCANTARA, M. A. M.
700 1 $aMORAES, M. L. T.
700 1 $aSILVA, E. A. A.
700 1 $aPEREIRA NETO, L. G.
700 1 $aSEBBENN, A. M.
700 1 $aROSSINI, B. C.
700 1 $aMARINO, C. L.
773 $tMolecular Biology Reports, 2021.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
16/04/2018 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LEAO, N. V. M.; FELIPE, S. H. S.; EMÍDIO-SILVA, C.; MORAES, A. C. dos S.; SHIMIZU, E. S. C.; GALLO, R.; FREITAS, A. D. D. de; KATO, O. R. |
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Afiliação: |
NOEMI VIANNA MARTINS LEAO, CPATU; Sérgio Heitor Sousa Felipe, UFV; Cláudio Emídio-Silva, UFPA; Antônia Camila dos Santos Moraes, UFRA; ELIZABETH SANTOS CORDEIRO SHIMIZU, CPATU; Ricardo Gallo, UFV; Alessandra Doce Dias de Freitas, UFPA; OSVALDO RYOHEI KATO, CPATU. |
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Título: |
Morphometric diversity between fruits and seeds of mahogany trees (Swietenia macrophylla King.) from Parakanã Indigenous Land, Pará State, Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 12, n. 03, p. 435-443, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Morphometric studies of fruits and forest seeds may indicate the presence of variability among individuals of the same species. The objective of this study was to investigate the morphometric diversity of fruits and seeds of Swietenia macrophylla in 20 individuals belong to the Parakanã Indigenous Land, PA, Brazil. The following characteristics of the fruits, wingless seeds, and seeds without testa of the 20 trees were evaluated. Analysis of variance was performed on the collected data, followed by test of the means by Scott-Knott test. Genetic dissimilarity was assessed through means of the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) grouping technique and canonical variables (CV). The results indicate high phenotypic diversity among mahogany fruits and seeds and the grouping of trees regardless is of the location of the indigenous village. Thus, mahogany trees have high genetic potential for germplasm conservation, seed harvesting, and breeding programs. |
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Palavras-Chave: |
Análise multivariada; Biometria de frutos e sementes; Conservação genética; Diversidade fenotípica; Sementes florestais. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/175480/1/leao-12-3-2018-435-443.pdf
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Marc: |
LEADER 01878naa a2200265 a 4500
001 2090614
005 2020-12-28
008 2018 bl uuuu u00u1 u #d
100 1 $aLEAO, N. V. M.
245 $aMorphometric diversity between fruits and seeds of mahogany trees (Swietenia macrophylla King.) from Parakanã Indigenous Land, Pará State, Brazil.$h[electronic resource]
260 $c2018
520 $aMorphometric studies of fruits and forest seeds may indicate the presence of variability among individuals of the same species. The objective of this study was to investigate the morphometric diversity of fruits and seeds of Swietenia macrophylla in 20 individuals belong to the Parakanã Indigenous Land, PA, Brazil. The following characteristics of the fruits, wingless seeds, and seeds without testa of the 20 trees were evaluated. Analysis of variance was performed on the collected data, followed by test of the means by Scott-Knott test. Genetic dissimilarity was assessed through means of the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) grouping technique and canonical variables (CV). The results indicate high phenotypic diversity among mahogany fruits and seeds and the grouping of trees regardless is of the location of the indigenous village. Thus, mahogany trees have high genetic potential for germplasm conservation, seed harvesting, and breeding programs.
653 $aAnálise multivariada
653 $aBiometria de frutos e sementes
653 $aConservação genética
653 $aDiversidade fenotípica
653 $aSementes florestais
700 1 $aFELIPE, S. H. S.
700 1 $aEMÍDIO-SILVA, C.
700 1 $aMORAES, A. C. dos S.
700 1 $aSHIMIZU, E. S. C.
700 1 $aGALLO, R.
700 1 $aFREITAS, A. D. D. de
700 1 $aKATO, O. R.
773 $tAustralian Journal of Crop Science$gv. 12, n. 03, p. 435-443, 2018.
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Embrapa Amazônia Oriental (CPATU) |
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