Data/hora: 10/06/2024 15:03:13

Biblioteca(s): | Embrapa Milho e Sorgo; Embrapa Solos.
Data corrente: | 14/03/2000
Data da última atualização: | 25/08/2023
Autoria: | OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; SOBRAL, B. W. S.; GUIMARAES, C. T.; ELSAS, J. D. van; MANFIO, G. P.
Afiliação: | V. M. DE OLIVEIRA, FUNDAÇÃO ANDRÉ TOSELLO; HEITOR LUIZ DA COSTA COUTINHO, CNPS; B. W. S. SOBRAL, NATIONAL CENTER FOR GENOME RESOURCES; C. T. GUIMARÃES, NATIONAL CENTER FOR GENOME RESOURCES; J. D. VAN ELSAS, RESEARCH INSTITUTE FOR PLANT PROTECTION; GILSON PAULO MANFIO, FUNDAÇÃO ANDRÉ TOSELLO.
Título: | Discrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE).
Ano de publicação: | 1999
Fonte/Imprenta: | Letters in Applied Microbiology, v. 28, N. 2, p. 137-141, Feb. 1999.
DOI: | https://doi.org/10.1046/j.1365-2672.1999.00480.x
Idioma: | Inglês
Conteúdo: | With the aim of detecting Rhizobium species directly in the environment, specific PCR primers for Rh, tropici and Rh, leguminosarum were designed on the basis of sequence analysis of 16S-23S rDNA spacer regions of several Rh. tropici, Rh. leguminosarum and Agrobacterium rhizogenes strains. Primer specificity was checked by comparison with available rDNA spacer sequences in databases, and by PCR using DNA from target and reference strains. Sequence polymorphisms of rDNA spacer fragments among strains of the same species were detected by denaturing gradient gel electrophoresis (DGGE). The specific PCR primers designed in the study could be applied to evaluate the diversity of Rh. tropici and Rh. leguminosarum by analysing the polymorphisms of 16S-23S spacer rDNA amplified from either whole-cell or soil-extracted DNA.
Palavras-Chave: | Analysis; Database; Diversidade; Diversity; tropici.
Thesagro: | Base de Dados; DNA; Eletroforese; Rhizobium; Solo.
Thesaurus Nal: | electrophoresis; soil.

Categoria do assunto:

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P Recursos Naturais, Ciências Ambientais e da Terra

Registro original: | Embrapa Solos (CNPS)

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CNPS - (8426-1) - UPC - AP - DD -  -  - 1999.00046 - DIS
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