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| Registros recuperados : 37 | |
| 13. |  | SCHIAVON, R. de A.; MORÁS, A.; PARAGINSKI, R. T.; RUTZ, D.; LORINI, I.; ELIAS, M. C. Efeito da secagem e do beneficiamento na preferência de consumo do inseto Sitophilus sp. In: CONFERÊNCIA BRASILEIRA DE PÓS-COLHEITA, 5., 2010, Foz do Iguaçu. Anais ... Londrina: Abrapós, 2010. p. 223-227. il. Organizado por Irineu Lorini, Maria Cristina Zborowski de Paula, Adriano Divino Lima Afonso.| Biblioteca(s): Embrapa Soja. |
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| 19. | | MORÁS, A.; RUTZ, D.; PINO, M.; BOHN, A.; LORINI, I.; ELIAS, M. C. Uso de terra de diatomácea e ácido propiônico como alternativa para controle de insetos em arroz armazenado. In: SIMPÓSIO SUL-BRASILEIRO DE QUALIDADE DE ARROZ, 3., 2008, Camaquã. Anais... Pelotas: Abrapós: Universidade Federal de Pelotas, 2008. p. 171-178.| Biblioteca(s): Embrapa Trigo. |
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| 20. | | MOURA, F. A. de; SPIER, F.; ZAVAREZE, E. da R.; DIAS, A. R. G.; ELIAS, M. C. Biscoitos tipo "cookie" elaborados com diferentes frações de semente de abóbora (curcubita máxima). Alimentos e nutrição, Araraquara, v. 21, n. 4, p. 579-585, out./dez. 2010.| Biblioteca(s): Embrapa Hortaliças. |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2013 |
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Data da última atualização: |
14/04/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, M. S. da; MONTEIRO, J. P.; NUNES, V. S.; VASCONCELOS, E. J.; PEREZ, A. M.; FREITAS JÚNIOR, L. de H.; ELIAS, M. C. |
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Afiliação: |
Marcelo Santos da Silva, Instituto de Biociências, Universidade Estadual Paulista (UNESP) - Botucatu, São Paulo, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; Vinícius Santana Nunes, UNESP - Botucatu, São Paulo, Brazil; Elton José Vasconcelos, Seattle Biomedical Research Institute - Seattle, Washington, United States of America; Arina Marina Perez, UNESP - Botucatu, São Paulo, Brazil; Lúcio de Holanda Freitas-Júnior, Laboratório Nacional de Biociências (LNBio), Centro Nacional de Pesquisa em Energia e Materiais - Campinas, São Paulo, Brazil; Maria Carolina Elias, Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan - São Paulo, SP, Brazil. |
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Título: |
Leishmania amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation during cell cycle. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
PLoS ONE, San Francisco, v. 8, n. 11, e81397, Nov. 2013. |
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DOI: |
10.1371/journal.pone.0081397 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus. MenosAbstract: Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the... Mostrar Tudo |
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Palavras-Chave: |
Cinetoplasto; Divisão celular; Leishmaniose tegumentar; Núcleo celular; Replicação; Replication. |
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Thesagro: |
Doença animal; Fisiologia; Leishmaniose. |
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Thesaurus NAL: |
Cell division; cell nucleus; DNA probes; Kinetoplastida; Leishmania; Leishmania amazonensis; leishmaniasis; Physiology. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/93811/1/API-Leishmania-amazonensis.pdf
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Marc: |
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008 2013 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0081397$2DOI
100 1 $aSILVA, M. S. da
245 $aLeishmania amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation during cell cycle.$h[electronic resource]
260 $c2013
520 $aAbstract: Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus.
650 $aCell division
650 $acell nucleus
650 $aDNA probes
650 $aKinetoplastida
650 $aLeishmania
650 $aLeishmania amazonensis
650 $aleishmaniasis
650 $aPhysiology
650 $aDoença animal
650 $aFisiologia
650 $aLeishmaniose
653 $aCinetoplasto
653 $aDivisão celular
653 $aLeishmaniose tegumentar
653 $aNúcleo celular
653 $aReplicação
653 $aReplication
700 1 $aMONTEIRO, J. P.
700 1 $aNUNES, V. S.
700 1 $aVASCONCELOS, E. J.
700 1 $aPEREZ, A. M.
700 1 $aFREITAS JÚNIOR, L. de H.
700 1 $aELIAS, M. C.
773 $tPLoS ONE, San Francisco$gv. 8, n. 11, e81397, Nov. 2013.
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