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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
15/07/2024 |
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Data da última atualização: |
16/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HELLER, L. M.; BASTOS, T. de S. A.; ZAPA. D. M. B.; MORAIS, I. M. L. de; SALVADOR, V. F.; LEAL, L. L. L. L.; COUTO, L. F. M.; NEVES, L. C.; PAULA, W. V. de F.; FERREIRA, L. L.; BARROS, A. T. M. de; CANCADO, P. H. D.; MACHADO, R. Z.; SOARES, V. E.; CADIOLI, F. A.; KRAWCZAK, F. da S.; LOPES, W. D. Z. |
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Afiliação: |
LUCIANA MAFFINI HELLER, UNIVERSIDADE FEDERAL DE GOIÁS; THIAGO DE SOUZA AZEREDO BASTOS, FACULDADE ANHANGUERA; DINA MARÍA BELTRÁN ZAPA, UNIVERSIDADE FEDERAL DE GOIÁS; IGOR MACIEL LOPES DE MORAIS, UNIVERSIDADE FEDERAL DE GOIÁS; VANESSA FERREIRA SALVADOR, UNIVERSIDADE FEDERAL DE GOIÁS; LUCCAS LOURENZZO LIMA LINS LEAL, UNIVERSIDADE FEDERAL DE GOIÁS; LUIZ FELLIPE MONTEIRO COUTO, UNIVERSIDADE FEDERAL DE GOIÁS; LUCIANNE CARDOSO NEVES, UNIVERSIDADE FEDERAL DE GOIÁS; WARLEY VIEIRA DE FREITAS PAULA, UNIVERSIDADE FEDERAL DE GOIÁS; LORENA LOPES FERREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ANTONIO THADEU MEDEIROS DE BARROS, CNPGC; PAULO HENRIQUE DUARTE CANCADO, CNPGC; ROSANGELA ZACARIAS MACHADO, UNIVERSIDADE ESTADUAL PAULISTA; VANDO EDÉSIO SOARES, UNIVERSIDADE BRASIL; FABIANO ANTONIO CADIOLI, UNIVERSIDADE ESTADUAL PAULISTA; FELIPE DA SILVA KRAWCZAK, UNIVERSIDADE FEDERAL DE GOIÁS; WELBER DANIEL ZANETTI LOPES, UNIVERSIDADE FEDERAL DE GOIÁS. |
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Título: |
Evaluation of mechanical transmission of Trypanosoma vivax by Stomoxys calcitrans in a region without a cyclic vector. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Parasitology Research, v. 123, issue 1, article number 96, 2024. |
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ISSN: |
1432-1955 (online) |
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DOI: |
https://doi.org/10.1007/s00436-023-08102-z |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - This work investigated the mechanical transmission of Trypanosoma vivax by Stomoxys calcitrans to cattle in a region without a cyclic vector. The study involved two experiments, one with calves experimentally infected with T. vivax, in the acute phase of trypanosomosis (Experiment 1) and the other in the chronic phase (Experiment 2). In both experiments, two transmission methods were used with flies that had not fed for 24 h or had never fed: (i) Method 1: flies released freely in cattle pens (≈3,300 flies/pen for 10 days); and (ii) Method 2: flies placed in a feeding chamber (12 flies/animal). To develop Method 1 in the two experiments (acute and chronic phases), T. vivax-positive animals were kept with T. vivax-negative animals. Periodically, the Brener method, Woo method, blood smears, cPCR, ELISA, IFAT, and Imunoteste® were performed to detect T. vivax in the animals. We also recorded the animals’ head tossing and hoof stomping and the number of flies near the pens’ inner walls. Subsequently, biological testing was performed using lambs. For Method 2 in both experiments, flies inside the feeding chamber first fed on T. vivax-positive animals and later on negative animals. In both experiments and methods, we examined the flies for the presence of T. vivax through blood smears and cPCR of the proboscis and abdomen. In Experiment 2 (chronic phase), a test was conducted to determine how long trypomastigotes forms could survive on the blood of animals with different levels of parasitemia. None of the animals (calves and lambs) became infected with T. vivax or showed antibodies against it. During the evaluation period, the animals in the presence of the flies exhibited more hoof stomping and head tossing compared to those without flies (control). Additionally, there was an increase in the number of flies in the pens during the experiment. Only in Experiment 1 (acute phase) were T. vivax trypomastigotes and DNA found in the abdomen of the flies but not in the proboscis. In Experiment 2 (chronic phase), higher concentrations of trypomastigotes per milliliter of blood were associated with a shorter the lifespan of this stage of the parasite. In conclusion, under the variable conditions of the experiments (hosts, number of flies, and level of parasitemia), S. calcitrans was unable to mechanically transmit T. vivax to cattle. MenosABSTRACT - This work investigated the mechanical transmission of Trypanosoma vivax by Stomoxys calcitrans to cattle in a region without a cyclic vector. The study involved two experiments, one with calves experimentally infected with T. vivax, in the acute phase of trypanosomosis (Experiment 1) and the other in the chronic phase (Experiment 2). In both experiments, two transmission methods were used with flies that had not fed for 24 h or had never fed: (i) Method 1: flies released freely in cattle pens (≈3,300 flies/pen for 10 days); and (ii) Method 2: flies placed in a feeding chamber (12 flies/animal). To develop Method 1 in the two experiments (acute and chronic phases), T. vivax-positive animals were kept with T. vivax-negative animals. Periodically, the Brener method, Woo method, blood smears, cPCR, ELISA, IFAT, and Imunoteste® were performed to detect T. vivax in the animals. We also recorded the animals’ head tossing and hoof stomping and the number of flies near the pens’ inner walls. Subsequently, biological testing was performed using lambs. For Method 2 in both experiments, flies inside the feeding chamber first fed on T. vivax-positive animals and later on negative animals. In both experiments and methods, we examined the flies for the presence of T. vivax through blood smears and cPCR of the proboscis and abdomen. In Experiment 2 (chronic phase), a test was conducted to determine how long trypomastigotes forms could survive on the blood of animals with differen... Mostrar Tudo |
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Thesagro: |
Parasito de Animal; Stomoxys Calcitrans; Trypanosoma Vivax. |
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Thesaurus Nal: |
Parasites; Proboscis; Trypomastigotes; Vector competence. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
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001 2165804
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022 $a1432-1955 (online)
024 7 $ahttps://doi.org/10.1007/s00436-023-08102-z$2DOI
100 1 $aHELLER, L. M.
245 $aEvaluation of mechanical transmission of Trypanosoma vivax by Stomoxys calcitrans in a region without a cyclic vector.$h[electronic resource]
260 $c2024
520 $aABSTRACT - This work investigated the mechanical transmission of Trypanosoma vivax by Stomoxys calcitrans to cattle in a region without a cyclic vector. The study involved two experiments, one with calves experimentally infected with T. vivax, in the acute phase of trypanosomosis (Experiment 1) and the other in the chronic phase (Experiment 2). In both experiments, two transmission methods were used with flies that had not fed for 24 h or had never fed: (i) Method 1: flies released freely in cattle pens (≈3,300 flies/pen for 10 days); and (ii) Method 2: flies placed in a feeding chamber (12 flies/animal). To develop Method 1 in the two experiments (acute and chronic phases), T. vivax-positive animals were kept with T. vivax-negative animals. Periodically, the Brener method, Woo method, blood smears, cPCR, ELISA, IFAT, and Imunoteste® were performed to detect T. vivax in the animals. We also recorded the animals’ head tossing and hoof stomping and the number of flies near the pens’ inner walls. Subsequently, biological testing was performed using lambs. For Method 2 in both experiments, flies inside the feeding chamber first fed on T. vivax-positive animals and later on negative animals. In both experiments and methods, we examined the flies for the presence of T. vivax through blood smears and cPCR of the proboscis and abdomen. In Experiment 2 (chronic phase), a test was conducted to determine how long trypomastigotes forms could survive on the blood of animals with different levels of parasitemia. None of the animals (calves and lambs) became infected with T. vivax or showed antibodies against it. During the evaluation period, the animals in the presence of the flies exhibited more hoof stomping and head tossing compared to those without flies (control). Additionally, there was an increase in the number of flies in the pens during the experiment. Only in Experiment 1 (acute phase) were T. vivax trypomastigotes and DNA found in the abdomen of the flies but not in the proboscis. In Experiment 2 (chronic phase), higher concentrations of trypomastigotes per milliliter of blood were associated with a shorter the lifespan of this stage of the parasite. In conclusion, under the variable conditions of the experiments (hosts, number of flies, and level of parasitemia), S. calcitrans was unable to mechanically transmit T. vivax to cattle.
650 $aParasites
650 $aProboscis
650 $aTrypomastigotes
650 $aVector competence
650 $aParasito de Animal
650 $aStomoxys Calcitrans
650 $aTrypanosoma Vivax
700 1 $aBASTOS, T. de S. A.
700 1 $aZAPA. D. M. B.
700 1 $aMORAIS, I. M. L. de
700 1 $aSALVADOR, V. F.
700 1 $aLEAL, L. L. L. L.
700 1 $aCOUTO, L. F. M.
700 1 $aNEVES, L. C.
700 1 $aPAULA, W. V. de F.
700 1 $aFERREIRA, L. L.
700 1 $aBARROS, A. T. M. de
700 1 $aCANCADO, P. H. D.
700 1 $aMACHADO, R. Z.
700 1 $aSOARES, V. E.
700 1 $aCADIOLI, F. A.
700 1 $aKRAWCZAK, F. da S.
700 1 $aLOPES, W. D. Z.
773 $tParasitology Research$gv. 123, issue 1, article number 96, 2024.
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| 1. |  | BRONDI, M.; EISA, M.; BORTOLETTO-SANTOS, R.; DRAPANAUSKAITE, D.; REEDDINGTON, T.; WILLIAMS, C.; RIBEIRO, C.; BALTRUSAITIS, J. Recovering, Stabilizing, and Reusing Nitrogen and Carbon from Nutrient-Containing Liquid Waste as Ammonium Carbonate Fertilizer. Agriculture, v. 13, n. 909, 2023. 28 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
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