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| Registros recuperados : 49 | |
| 23. |  | SILVA, C. M. da; VASCONCELOS, S. S.; RAMOS, E. M. L. S.; CAPELA, C. Relação entre fatores abióticos e emissão de CO2 em sistemas agroflorestais com dendê em Tomé-Açu - PA. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD-ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 25. | | VESCHI, J. L. A.; MARTINS, R. J.; ZAFALON, L. F.; RAMOS, E. M.; CASTRO, R. S. Soroprevalência da CAE em caprinos leiteiros do território do sisal, Bahia. Biológico, v. 73, (Supl. 2), p. 25, 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 26. | | SILVA, C. M. da; VASCONCELOS, S. S.; RAMOS, E. M. L. S.; CAPELA, C. Variação temporal do efluxo de dióxido de carbono pelo solo em sistemas de cultivo de dendê consorciado em Tomé-Açu, PA. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 8., 2011, Belém, PA. Anais... Belém, PA: SBSAF: Embrapa Amazônia Oriental: UFRA: CEPLAC: EMATER: ICRAF, 2011. 1 CD-ROM. Editores técnicos: Roberto Porro, Milton Kanashiro, Maria do Socorro Gonçalves Ferreira, Leila Sobral Sampaio e Gladys Ferreira de Sousa.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 30. | | MARTINS, R. de J.; VESCHI, J. L. A.; RAMOS, E. M.; FREITAS, M. do C.; ATAÍDE, J. Avaliação da ocorrência de anticorpos Anti-brucella abortus em caprinos da região semiárida do Estado de Pernambuco. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 4., 2009, Petrolina. Anais... Petrolina: Embrapa Semi-Árido, 2009. p. 268-272. (Embrapa Semi-Árido. Documentos, 221).| Biblioteca(s): Embrapa Semiárido. |
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| 34. | | VESCHI, J. L. A.; LANDIM, A. M. de S.; RAMOS, E. M.; ZAFALON, L. F.; CASTRO, R. S. de. Soroprevalência de lentiviroses de pequenos ruminantes em ovinos e caprinos na região do submédio do Vale do São Francisco, Pernambuco, Brasil. Acta Scientiae Veterinariae, v. 40, p. s99, 2012. Suplemento 2. Edição do 1 Encontro Nacional de Epidemiologia Veterinária - ENEPI, São Paulo, jul. 2012.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| 35. | | ROCHA, F. E. de C.; RAMOS, E. M. S.; KISHI, S. M.; MARCELINO, M. Q. dos S.; SOUSA, E. dos S. de; JÚLIO, L. DE; TIMPONI, M. R. Avaliação qualitativa da percepção de extensionistas rurais em relação ao uso e à conservação de recursos hídricos. Planaltina, DF: Embrapa Cerrados, 2010. 46 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 297).| Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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| 37. | | VESCHI, J. L. A.; ZAFALON, L. F.; RAMOS, E. M.; OKUDA, L. H.; STEFANO, E. de; NOGUEIRA, A. H. C.; PITUCO, E. M. Ovinos portadores de anticorpos para o vírus da língua azul oriundos do Rio Grande do Sul para o Semiárido pernambucano. O Biológico, v. 75, p. 29, 2013. Suplemento 2, ref. 21. Edição dos Resumos do 3 Encontro Nacional de Defesa Sanitária Animal, Foz de Iguaçu, dez. 2013.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| 38. | | VESCHI, J. L. A.; ZAFALON, L. F.; RAMOS, E. M.; OKUDA, L. H.; STEFANO, E.; NOGUEIRA, A. H. C.; RIBEIRO, C. P.; PITUCO, E. M. Ocorrência de animais portadores de anticorpos para o vírus da Língua Azul no semiárido pernambucano. In: CONFERÊNCIA NACIONAL DE DEFESA AGROPECUÁRIA, 5., 2014, Florianópolis. Defesa agropecuária a serviço de um Estado de excelência: anais. Florianópolis: Sociedade Brasileira de Defesa Agropecuária; Concórida: Embrapa Suínos e Aves, 2014. p. 61.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| 39. | | SILVA, V. A.; BERTECHINI, A. G.; CARVALHO, A. C. de; CASTRO, R. T. da C.; OLIVEIRA, B. L. de; KONIG, I. F. M.; RAMOS, E. M. Meat quality and performance of broilers fed diets containing selenium yeast and sodium selenite. Pesquisa Agropecuária Brasileira, v. 57, e02428, 2022. Título em português: Qualidade da carne e desempenho de frangos de corte alimentados com dietas contendo selênio levedura e selenito de sódio.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 40. | | ALBUQUERQUE, M. P. F. de; MORAE4S, F. K. C.; SANTOS, R. I. N.; CASTRO, G. L. S. de; RAMOS, E. M. L. S.; PINHEIRO, H. A. Ecofisiologia de plantas jovens de mogno-africano submetidas a deficit hídrico e reidratação. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 1, p. 9-16, jan. 2013. Título em inglês: Ecophysiology of young African mahogany plants subjected to water deficit and rewetting.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 49 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/11/2015 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; CABRAL, E. C.; COELHO, M. B.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. |
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Afiliação: |
UNESP; UNESP; UNICAMP; UNICAMP; UNICAMP; UNICAMP; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP. |
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Título: |
Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Theriogenology, v. 84, n. 1, p. 127-136, 2015. |
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DOI: |
10.1016/j.theriogenology.2015.02.023 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species. MenosThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on... Mostrar Tudo |
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Palavras-Chave: |
Animal embryos; Bovine embryo; Cryosurvival; In vitro production; Lipid profile. |
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Thesagro: |
Bovino; Embrião animal; Reprodução animal. |
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Thesaurus NAL: |
Cattle; conjugated linoleic acid; In vitro fertilization; mass spectrometry. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02882naa a2200373 a 4500
001 2029790
005 2016-03-17
008 2015 bl --- 0-- u #d
024 7 $a10.1016/j.theriogenology.2015.02.023$2DOI
100 1 $aLEÃO, B. C. S.
245 $aImproved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile.
260 $c2015
520 $aThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species.
650 $aCattle
650 $aconjugated linoleic acid
650 $aIn vitro fertilization
650 $amass spectrometry
650 $aBovino
650 $aEmbrião animal
650 $aReprodução animal
653 $aAnimal embryos
653 $aBovine embryo
653 $aCryosurvival
653 $aIn vitro production
653 $aLipid profile
700 1 $aROCHA-FRIGONI, N. A. S.
700 1 $aCABRAL, E. C.
700 1 $aCOELHO, M. B.
700 1 $aFERREIRA, C. R.
700 1 $aEBERLIN, M. N.
700 1 $aACCORSI, M. F.
700 1 $aNOGUEIRA, E.
700 1 $aMINGOTI, G. Z.
773 $tTheriogenology$gv. 84, n. 1, p. 127-136, 2015.
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