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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
10/01/2011 |
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Data da última atualização: |
15/10/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JONSSON, C. M.; PARAIBA, L. C.; AOYAMA, H. |
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Afiliação: |
CLAUDIO MARTIN JONSSON, CNPMA; LOURIVAL COSTA PARAIBA, CNPMA; Hiroshi Aoyama, Departamento de Bioquímica - UNICAMP. |
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Título: |
Algae acid phosphatase affected by ten metals and enzymatic parameters determination in presence of mercury and copper. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Pesticidas: Revista de Ecotoxicologia e Meio Ambiente, Curitiba, v. 19, p. 1-10, jan./dez. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Acid phosphatases play important roles in algae metabolism such availability/recycling of inorganic phosphate and autophagic digestive processes. Chemicals released into the environment from agricultural activities and through industrial and urban wastes, may impair algae enzyme activity. The aim of this work was to evaluate the in vitro activation/inhibition effect of ten metals, commonly present as contaminants in soil and water, on the acid phosphatase extracted from the green algae Pseudokirchneriella subcapitata. Results demonstrated that Hg, Al, Mo, Pb, Se and Cd inhibited the enzyme activity in 56.3, 54.5, 30.6, 25.5, 23.1 and 11.5%, respectively. This corresponds to the maximum percentage of effect attained at the metal concentrations tested (0.02?2.0 mM). On the other hand, Cu, Zn, Ni and Cr exhibited an increment on phosphatase activity equal to 95.5, 87.6, 77.6 and 42.8%, respectively. Kinetic parameters values were calculated for the metals that showed highest effects. Thus, Ki (inhibition constant) and Kd (dissociation constant) values equal to 0.0400 and 0.0016 mM were determined for Hg and Cu, respectively. A noncompetitive inhibition mechanism was attributed to the former. Results improved the understanding of the basic events of the impact of metals at biochemical levels in primary producers organisms. |
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Thesagro: |
Fitoplâncton; Fosfatase Ácida. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01901naa a2200169 a 4500
001 1872419
005 2015-10-15
008 2010 bl --- 0-- u #d
100 1 $aJONSSON, C. M.
245 $aAlgae acid phosphatase affected by ten metals and enzymatic parameters determination in presence of mercury and copper.
260 $c2010
520 $aAcid phosphatases play important roles in algae metabolism such availability/recycling of inorganic phosphate and autophagic digestive processes. Chemicals released into the environment from agricultural activities and through industrial and urban wastes, may impair algae enzyme activity. The aim of this work was to evaluate the in vitro activation/inhibition effect of ten metals, commonly present as contaminants in soil and water, on the acid phosphatase extracted from the green algae Pseudokirchneriella subcapitata. Results demonstrated that Hg, Al, Mo, Pb, Se and Cd inhibited the enzyme activity in 56.3, 54.5, 30.6, 25.5, 23.1 and 11.5%, respectively. This corresponds to the maximum percentage of effect attained at the metal concentrations tested (0.02?2.0 mM). On the other hand, Cu, Zn, Ni and Cr exhibited an increment on phosphatase activity equal to 95.5, 87.6, 77.6 and 42.8%, respectively. Kinetic parameters values were calculated for the metals that showed highest effects. Thus, Ki (inhibition constant) and Kd (dissociation constant) values equal to 0.0400 and 0.0016 mM were determined for Hg and Cu, respectively. A noncompetitive inhibition mechanism was attributed to the former. Results improved the understanding of the basic events of the impact of metals at biochemical levels in primary producers organisms.
650 $aFitoplâncton
650 $aFosfatase Ácida
700 1 $aPARAIBA, L. C.
700 1 $aAOYAMA, H.
773 $tPesticidas: Revista de Ecotoxicologia e Meio Ambiente, Curitiba$gv. 19, p. 1-10, jan./dez. 2009.
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Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 5 | |
| 2. |  | DYMINSKI, D. S.; TAKEDA, H. H.; OLIVEIRA, R.; BOLINI, H. M. A.; MATTOSO, L. H. C.; CÂNDIDO, L. M. B. The electronic tongue as instrument to sweeteners analysis: an innovation. In: INNOVATIONS IN TRADITIONAL FOODS, EFFOST, 2005, Valencia. Proceedings... Valencia: Elsevier, 2005. p. 197-199. v. 1.| Biblioteca(s): Embrapa Instrumentação. |
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| 3. | | DYMINSKI, D. S.; TAKEDA, H. H.; BOLINI, H. M. A.; MATTOSO, L. H.; CÃNDIDO, L. M. B. Utilização da língua eletrônica para avaliação de aromas. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DOS ALIMENTOS, 6., 2005, Campinas. Ciência dos alimentos> abrindo caminhos para o desenvolvimento científico, tecnológico e industrial. [Resumos...] Campinas: UNICAMP, 2005.| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | DYMINSKI, D. S.; BOFFO, E. F.; TAKEDA, H. H.; FERRREIRA, A. G.; MATTOSO, L. H. C.; CÂNDIDO, L. M. B. Safety evaluation of sweetener mixture using Nuclear Magnetic Resonance. In: INNOVATIONS IN TRADITIONAL FOODS, EFFOST, 2005, Valencia. Delegate manual. Valencia: Elsevier, 2005. [P2.09]| Biblioteca(s): Embrapa Instrumentação. |
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| 5. | | DYMINSKI, D. S.; PATERNO, L. G.; TAKEDA, H. H.; BOLINI, H. M. A.; MATTOSO, L. H. C.; CÂNDIDO, L. M. B. Correlation between human panel and electronic tongue responses on the analysis of commercial sweeteners. Sensor letters, v. 4, n. 4, p. 403-408, 2006.| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 5 | |
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