Data/hora: 09/06/2024 04:10:39

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 19/10/2018
Data da última atualização: | 07/02/2023
Autoria: | MASCARIN, G. M.; KOBORI, N. N.; JACKSON, M. A.; DUNLAP, C. A.; DELALIBERA JUNIOR, I.
Afiliação: | GABRIEL MOURA MASCARIN, CNPMA; NILCE NAOMI KOBORI, USDA-ARS; MARK A JACKSON, USDA-ARS; CHRISTOPHER A DUNLAP, USDA-ARS; ITALO DELALIBERA JUNIOR, ESALQ-USP.
Título: | Nitrogen sources affect productivity, desiccation tolerance and storage stability of Beauveria bassiana blastospores.
Ano de publicação: | 2018
Fonte/Imprenta: | Journal of Applied Microbiology, v. 124, n. 3, p. 810-820, 2018.
DOI: | https://doi.org/10.1111/jam.1369
Idioma: | Inglês
Conteúdo: | Abstract: Aims: Nitrogen is a critical element in industrial fermentation media. This study investigated the influence of various nitrogen sources on blastospore production, desiccation tolerance and storage stability using two strains of the cosmopolitan insect-pathogenic fungus Beauveria bassiana. Methods and Results: Complex organic sources of nitrogen such as soy flour, autolysed yeast and cottonseed flour induced great numbers of blastospores after 2-3 days of fermentation, which also survived drying and remained viable (32-56% survival) after 9 months storage at 4°C, although variations were found between strains. Nitrogen availability in the form of free amino acids directly influenced blastospore production and resistance to desiccation. Increasing glucose and nitrogen concentrations up to 120 and 30 g l-1, respectively, did not improve blastospore production but enhanced desiccation tolerance. Cell viability after drying and upon fast-rehydration was increased when >25 g acid-hydrolysed casein per litre was supplemented in the liquid culture medium. Conclusions: These findings indicate that low-cost complex nitrogen compounds are suitable to enhance yeast-like growth by B. bassiana with good desiccation tolerance and therefore support its further scale-up production as a mycoinsecticide.
Palavras-Chave: | Air-drying; Biocontrol; Dimorphic growth; Liquid fermentation; Storage stability.
Thesagro: | Beauveria Bassiana; Fermentação Industrial; Fungo Para Controle Biológico.
Thesaurus Nal: | entomopathogenic fungi; Fermentation industry; Liquid state fermentation.
Categoria do assunto: | --
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16178-1) - UPC - AP - DD -  -  -  - DIS