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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Trigo. |
Data corrente: |
06/04/2023 |
Data da última atualização: |
01/03/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RIBEIRO, M. R.; SIMEONE, M. L. F.; TRINDADE, R. dos S.; DIAS, L. A. dos S.; GUIMARAES, L. J. M.; TIBOLA, C. S.; AZEVEDO, T. C. de. |
Afiliação: |
MARIANA RODRIGUES RIBEIRO, Universidade Federal de Viçosa; MARIA LUCIA FERREIRA SIMEONE, CNPMS; ROBERTO DOS SANTOS TRINDADE, CNPMS; LUIZ ANTONIO DOS SANTOS DIAS, Universidade Federal de Viçosa; LAURO JOSE MOREIRA GUIMARAES, CNPMS; CASIANE SALETE TIBOLA, CNPT; TACILA CRISTINA DE AZEVEDO, Universidade Federal de São João del-Rei. |
Título: |
Near infrared spectroscopy (NIR) and chemometrics methods to identification of haploids in maize. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Microchemical Journal, v. 190, 108604, 2023. |
DOI: |
https://doi.org/10.1016/j.microc.2023.108604 |
Idioma: |
Inglês |
Conteúdo: |
Haploids in maize are selected through visual analysis, whether by use of expression of the R1-navajo marker in seeds, or by the phenotype of haploid plants in the field. These methods of identification of haploid plants demand space, resources, and time. Near infrared spectroscopy (NIR), together with multivariate calibration methods, is an alternative for identification of haploid seeds and plants in a quick and accurate way. The aim of the present study was to use NIR to classify haploid and diploid seeds and differentiate haploid, doubled haploid, and diploid plants originating from different genotypes used as donor. In the first experiment, haploid and diploid maize seeds were analyzed from four genotypes used as parents. In another experiment, diploid, haploid, and doubled haploid plants from 34 source populations were evaluated and differentiated. Spectral data were collected from all the seed and leaf samples using the portable MicroNIR device. Then the data were preprocessed by principal component analysis and by partial least squares discriminant analysis (PLS-DA). The use of the portable MicroNIR, together with the PLS-DA method, enabled classification of haploid and diploid seeds and haploid, doubled haploid, and diploid plants with 100% accuracy, constituting a fast, simple, nondestructive, and reliable methodology for identification of haploids in maize, and it is more effective than the visual analysis. |
Palavras-Chave: |
Análise multivariada; Duplo haploide; Multivariate analisys; Spectroscopic techniques; Técnica espectroscópica. |
Thesagro: |
Milho; Zea Mays. |
Thesaurus Nal: |
Doubled haploids. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02371naa a2200301 a 4500 001 2153046 005 2024-03-01 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.microc.2023.108604$2DOI 100 1 $aRIBEIRO, M. R. 245 $aNear infrared spectroscopy (NIR) and chemometrics methods to identification of haploids in maize.$h[electronic resource] 260 $c2023 520 $aHaploids in maize are selected through visual analysis, whether by use of expression of the R1-navajo marker in seeds, or by the phenotype of haploid plants in the field. These methods of identification of haploid plants demand space, resources, and time. Near infrared spectroscopy (NIR), together with multivariate calibration methods, is an alternative for identification of haploid seeds and plants in a quick and accurate way. The aim of the present study was to use NIR to classify haploid and diploid seeds and differentiate haploid, doubled haploid, and diploid plants originating from different genotypes used as donor. In the first experiment, haploid and diploid maize seeds were analyzed from four genotypes used as parents. In another experiment, diploid, haploid, and doubled haploid plants from 34 source populations were evaluated and differentiated. Spectral data were collected from all the seed and leaf samples using the portable MicroNIR device. Then the data were preprocessed by principal component analysis and by partial least squares discriminant analysis (PLS-DA). The use of the portable MicroNIR, together with the PLS-DA method, enabled classification of haploid and diploid seeds and haploid, doubled haploid, and diploid plants with 100% accuracy, constituting a fast, simple, nondestructive, and reliable methodology for identification of haploids in maize, and it is more effective than the visual analysis. 650 $aDoubled haploids 650 $aMilho 650 $aZea Mays 653 $aAnálise multivariada 653 $aDuplo haploide 653 $aMultivariate analisys 653 $aSpectroscopic techniques 653 $aTécnica espectroscópica 700 1 $aSIMEONE, M. L. F. 700 1 $aTRINDADE, R. dos S. 700 1 $aDIAS, L. A. dos S. 700 1 $aGUIMARAES, L. J. M. 700 1 $aTIBOLA, C. S. 700 1 $aAZEVEDO, T. C. de 773 $tMicrochemical Journal$gv. 190, 108604, 2023.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
09/11/2018 |
Data da última atualização: |
25/02/2019 |
Autoria: |
PEREIRA, T. P.; MODESTO, E. C.; NEPOMUCENO, D. de D.; OLIVEIRA, O. F. de; FREITAS, R. S. X. de; MUIR, J. P.; DUBEUX JUNIOR, J. C. B.; ALMEIDA, J. C. de C. |
Afiliação: |
Tatiana Pires Pereira, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Elisa Cristina Modesto, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Delci de Deus Nepomuceno, In memoriam; Osniel Faria de Oliveira, Universidade Federal Rural de Pernambuco - UFRPE; Rafaela Scalise Xavier de Freitas, Universidade Federal Rural do Rio de Janeiro - UFRRJ; James Pierre Muir, Texas AgriLife Research and Extension Center; José Carlos Batista Dubeux Junior, University of Florida, North Florida Research and Education Center; João Carlos de Carvalho Almeida, Universidade Federal Rural do Rio de Janeiro - UFRRJ. |
Título: |
Characterization and biological activity of condensed tannins from tropical forage legumes. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 9, p. 1070-1077, Sept. 2018. |
Idioma: |
Inglês |
Notas: |
Título em português: Caracterização e atividade biológica de taninos condensados de leguminosas forrageiras tropicais. |
Conteúdo: |
The objective of this work was to characterize condensed tannins (CT) from six tropical forage legumes and to determine their biological activity. The monomers propelargonidin, prodelphinidin and procyanidin were analyzed, as well as extractable condensed tannin (ECT), protein-bound CT (PBCT) and fiber-bound CT (FBCT), molecular weight, degree of polymerization, polydispersity index, and biological activity by protein precipitate by phenols (PPP) of leaves of the legumes Cajanus cajan, Gliricidia sepium, Stylosanthes capitata x Stylosanthes macrocephala (stylo), Flemingia macrophylla, Cratylia argentea, and Mimosa caesalpiniifolia, and of the bark of this latter species. Differences were observed in the concentrations of ECT, PBCT, PPP, and total condensed tannin among species, but not in that of FBCT. The highest value of PBCT occurred in F. macrophylla. Total CT varied from nondetected concentration in C. argentea to the highest concentration in M. caesalpiniifolia leaves that contain the greatest levels of PPP. No differences were observed for polymerization degree in stylo, F. macrophylla, and M. caesalpiniifolia. Leaves of stylo, C. cajan, and G. sepium, all containing between 20 and 50 g kg-1 total CT, should be beneficial CT sources, if offered as sole feeds in ruminant diets. The ratio of prodelphinidin:procyanidin varies from 10:80 (stylo) to 65:35 (F. machrophylla), and propelargonidin is only determined in C. argentea. |
Palavras-Chave: |
Procianidina; Prodelfinidina; Prodelphinidins; Protein. |
Thesagro: |
Fenol; Leguminosa Forrageira; Proteína. |
Thesaurus NAL: |
Forage legumes; Phenols; Procyanidins. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/185728/1/Characterization-and-biological-activity.pdf
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Marc: |
LEADER 02551naa a2200337 a 4500 001 2099109 005 2019-02-25 008 2018 bl uuuu u00u1 u #d 100 1 $aPEREIRA, T. P. 245 $aCharacterization and biological activity of condensed tannins from tropical forage legumes.$h[electronic resource] 260 $c2018 500 $aTítulo em português: Caracterização e atividade biológica de taninos condensados de leguminosas forrageiras tropicais. 520 $aThe objective of this work was to characterize condensed tannins (CT) from six tropical forage legumes and to determine their biological activity. The monomers propelargonidin, prodelphinidin and procyanidin were analyzed, as well as extractable condensed tannin (ECT), protein-bound CT (PBCT) and fiber-bound CT (FBCT), molecular weight, degree of polymerization, polydispersity index, and biological activity by protein precipitate by phenols (PPP) of leaves of the legumes Cajanus cajan, Gliricidia sepium, Stylosanthes capitata x Stylosanthes macrocephala (stylo), Flemingia macrophylla, Cratylia argentea, and Mimosa caesalpiniifolia, and of the bark of this latter species. Differences were observed in the concentrations of ECT, PBCT, PPP, and total condensed tannin among species, but not in that of FBCT. The highest value of PBCT occurred in F. macrophylla. Total CT varied from nondetected concentration in C. argentea to the highest concentration in M. caesalpiniifolia leaves that contain the greatest levels of PPP. No differences were observed for polymerization degree in stylo, F. macrophylla, and M. caesalpiniifolia. Leaves of stylo, C. cajan, and G. sepium, all containing between 20 and 50 g kg-1 total CT, should be beneficial CT sources, if offered as sole feeds in ruminant diets. The ratio of prodelphinidin:procyanidin varies from 10:80 (stylo) to 65:35 (F. machrophylla), and propelargonidin is only determined in C. argentea. 650 $aForage legumes 650 $aPhenols 650 $aProcyanidins 650 $aFenol 650 $aLeguminosa Forrageira 650 $aProteína 653 $aProcianidina 653 $aProdelfinidina 653 $aProdelphinidins 653 $aProtein 700 1 $aMODESTO, E. C. 700 1 $aNEPOMUCENO, D. de D. 700 1 $aOLIVEIRA, O. F. de 700 1 $aFREITAS, R. S. X. de 700 1 $aMUIR, J. P. 700 1 $aDUBEUX JUNIOR, J. C. B. 700 1 $aALMEIDA, J. C. de C. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 53, n. 9, p. 1070-1077, Sept. 2018.
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