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| Registros recuperados : 11 | |
| 1. |  | DRUMOND, B. P.; LEITE, J. A.; FONSECA, F. G.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. Brazilian vaccinia virus strains are genetically divergent and differ from the lister vaccine strain. Microbes and Infection, Dorchester, v. 10, n. 2, p. 185-197, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | LEITE, J. A.; DRUMOND, B. P.; TRINDADE, G. de S.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. Brazilian vaccinia virus strains show genetic polymorphism at the ati gene. Virus Genes, v. 35, n. 3, p. 531-539, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | ASSIS, A. S. F.; CRUZ, L. T.; DRUMOND, B. P.; OTENIO, M. H.; VIEIRA, C. B.; MIAGOSTOVICH, M. P.; ROSA E SILVA, M. L. Detecção de rotavirus x parâmetros microbiológicos e físico-químicos de avaliação da qualidade da água. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... [Natal]: Sociedade Brasileira de Microbiologia, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | ASSIS, A. S. F.; OTENIO, M. H.; DOMINGUES, A. L. S.; DRUMOND, B. P.; MIOGOSTOVICH. M. P. P.; ROSA e SILVA, M. L. Environmental surveillance of human adenoviruses in a wastewater treatment plant. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 77., 2016, Pirenópolis. Anais... Brasília, DF: Sociedade Brasileira de Virologia, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | ASSIS, A. S. F.; CRUZ, L. T.; PINTO, M. A. DE O.; BESSA, M. A.; DRUMOND, B. P.; OTENIO, M. H.; VIEIRA, C. B.; MIAGOSTOVICH, M. P.; ROSA E SILVA, M. L. Relationship between microbiological and physico-chemical parametrs and occurrence of rotavirus genomes in drainage basin of Juiz de Fora, MG. Journal of the Brazilian Society for Virology, v. 17, n. 2, supl. 1, p. 117-118, 2012. Edição dos abstracts do 23º Brazilian Congress of Virology e 7º Mercosur Meeting of Virology, Foz do Iguaçu, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | ABRAHÃO, J. S.; FERNANDES, A. T. S.; ASSIS, F. L.; GUEDES, M. I.; DRUMOND, B. P.; LEITE, J. de A.; COELHO, L. F. L.; TURRINI, F.; FONSECA, F. G.; LOBATO, Z. I. P.; MADUREIRA, M.; FERREIRA, P. C.; BONJARDIM, C. A.; TRINDADE, G. S.; KROON, E. G. Human vaccinia virus and pseudocowpox virus co-infection: clinical description and phylogenetic characterization. Journal of Clinical Virology, v. 48, p. 69-72, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | ARAUJO, F.; DRUMOND, B.; ZERLOTINI, A.; ROSE, I. C.; LOPES, B. C.; GUEDES, E.; ARBEX, W. A.; MACHADO, M. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; GUIMARAES, M. F. M.; COIMBRA, R. S.; CARVALHO, M. R. S.; SILVA, M. V. G. B.; OLIVEIRA, G. C. Zebu genome sequencing and analysis using second generation sequencers. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | ZERLOTINI, A.; ARAÚJO, F.; DRUMOND, B.; ROSSE, I.; CUADROS-ORELLANA, S.; LOPEZ, B.; GUEDES, E.; ARBEX, W. A.; MACHADO, M. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; MARTINS, M. F.; COIMBRA, R.; CARVALHO, M. R.; SILVA, M. V. G. B.; OLIVEIRA, G. Zebu genome sequencing and analysis using second generation technology. In: Annual International Conference on Intelligent Systems for Molecular Biology, 19., 2011, Vienna. Proceedings...| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | TRINDADE, G. de S.; DRUMOND, B. P.; GUEDES, M. I. M. C.; LEITE, J. A.; MOTA, B. E. F.; CAMPOS, M. A.; FONSECA, F. G. da; NOGUEIRA, M. L.; LOBATO, Z. I. P.; BONJARDIM, C. A.; FERREIRA, P. C. P.; KROON, E. G. Zoonotic vaccinia virus infection in Brazil: clinical description and implications for health professionals. Journal of Clinical Microbiology, v. 45, n. 4., p. 1370-1372, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | MACHADO, T. B.; ANDRADE, A. C. dos S. P.; DORNAS, F. P.; MARINHO, P. E. S.; REIS, E. V. de S.; SILVA, N. I. O. da; AQUINO, I. L. M. de; MENDONÇA, D. C.; DRUMOND, B. P.; BORATTO, P. V. de M.; PICORELLI, A. C. R.; FIGUEIREDO, P. de O.; ANDRADE, A. de S.; ARANTES, T. S.; BERGIER, I.; COMERLADO, J.; KROON, E. G.; ABRAHÃO, J. S. Prospecção de vírus gigantes em biomas brasileiros. In: SIMPÓSIO DE MICROBIOLOGIA DA UFMG, 5., 2018, Belo Horizonte. Resumos... Belo Horizonte: Universidade Federal de Minas Gerais, 2018. p. 151.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 11 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
10/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ASSIS, A. S. F.; OTENIO, M. H.; DRUMOND, B. P.; FUMIAN, T. M.; MIAGOSTOVICH, M. P.; SILVA, M. L. da R. e. |
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Afiliação: |
Andrêssa S.F.Assis, UFJF; MARCELO HENRIQUE OTENIO, CNPGL; Betânia P. Drumond, UFJF; Tulio M. Fumian, Instituto Oswaldo Cruz; Marize P Miagostovich, Instituto Oswaldo Cruz; Maria Luzia da Rosa e Silva, UFJF. |
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Título: |
Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Science of The Total Environment, v. 583, p. 163-168, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories. MenosAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really... Mostrar Tudo |
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Palavras-Chave: |
Human adenovirus; Internal control; Organic flocculation; Quantitative PCR; Wastewater treatment plant. |
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Thesaurus NAL: |
sewage sludge. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02366naa a2200253 a 4500
001 2084982
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
100 1 $aASSIS, A. S. F.
245 $aOptimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge.$h[electronic resource]
260 $c2017
520 $aAbstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories.
650 $asewage sludge
653 $aHuman adenovirus
653 $aInternal control
653 $aOrganic flocculation
653 $aQuantitative PCR
653 $aWastewater treatment plant
700 1 $aOTENIO, M. H.
700 1 $aDRUMOND, B. P.
700 1 $aFUMIAN, T. M.
700 1 $aMIAGOSTOVICH, M. P.
700 1 $aSILVA, M. L. da R. e
773 $tScience of The Total Environment$gv. 583, p. 163-168, 2017.
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