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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
27/07/2009 |
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Data da última atualização: |
26/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENNA, P.; PEREIRA, A. A.; BANGEL, E. V.; HUNGRIA, M. |
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Afiliação: |
PÂMELA MENNA, UEL/CNPq-MCT; ALAN ALVES PEREIRA, CNPq-MCT; ELIANE VILLAMIL BANGEL, FEPAGRO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Symbiosis, Philadelphia, v. 48, n. 1-3, p. 120-130, Feb. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
With more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies. |
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Thesagro: |
Bactéria; Fixação de Nitrogênio; Inoculante; Taxonomia. |
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Thesaurus Nal: |
Inoculation methods; Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01873naa a2200229 a 4500
001 1471352
005 2024-06-26
008 2009 bl uuuu u00u1 u #d
100 1 $aMENNA, P.
245 $aRep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool.$h[electronic resource]
260 $c2009
520 $aWith more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies.
650 $aInoculation methods
650 $aNitrogen fixation
650 $aBactéria
650 $aFixação de Nitrogênio
650 $aInoculante
650 $aTaxonomia
700 1 $aPEREIRA, A. A.
700 1 $aBANGEL, E. V.
700 1 $aHUNGRIA, M.
773 $tSymbiosis, Philadelphia$gv. 48, n. 1-3, p. 120-130, Feb. 2009.
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Embrapa Soja (CNPSO) |
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| 2. |  | LIMA, E. A. de; COELHO, F. C.; LIMA, A. A. de; DORNELLES, M. S.; GARCIA, R. F. Semeadura direta de soja sobre diferentes plantas de cobertura e manejos de palhada, na região norte fluminense. Revista Ceres, Viçosa, MG, v. 56, n. 6, p. 790-795, nov./dez. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
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