Data/hora: 21/06/2024 03:34:13

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 08/01/2020
Data da última atualização: | 06/02/2024
Autoria: | DORNELES, E. M. S.; FONSECA, M. D. A. M.; ABREU, J. A. P.; LAGE, A. P.; BRITO, M. A. V. P.; PEREIRA, C. R.; BRANDAO, H. de M.; GUIMARÃES, A. S.; HEINEMANN, M. B.
Afiliação: | Elaine M. S. Dorneles; Mariana D. A. M. Fonseca; Juliana A. P. Abreu; Andrey P. Lage; Maria A. V. P. Brito; Carine R. Pereira; HUMBERTO DE MELLO BRANDAO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; Marcos B. Heinemann.
Título: | Genetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil.
Ano de publicação: | 2019
Fonte/Imprenta: | Microbiology Open, v. 8, n. 5, article e736, 2019.
DOI: | https://doi.org/10.1002/mbo3.736
Idioma: | Inglês
Conteúdo: | The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed&#8208;field gel electrophoresis (PFGE) genotypes. Seventy&#8208;nine isolates were used, including S. aureus (n = 71) and coagulase&#8208;negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve S. aureus and four CoNS were classified as multidrug resistance strains. Percentage of MRSA was also higher among CoNS (75%), compared to S. aureus isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum&#8208;spanning tree (MST) analysis with data from municipalities, herds, methicillin&#8208;resistant S. aureus (MRSA), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among CoNS, and a high genetic diversity among Staphylococcus spp. isolated from dairy cows with mastitis in Minas Gerais, Brazil.
Palavras-Chave: | Antimicrobial resistance; Coagulase-negative staphylococci; Methicillin-resistant S aureus; PFGE; Staphylococci.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/208347/1/Dorneles-et-al-2019-MicrobiologyOpen.pdf
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (24950-1) - UPC - AP - DD -  -  -  - DIS