Data/hora: 11/06/2024 22:36:33

Biblioteca(s): 	Embrapa Pecuária Sudeste; Embrapa Pecuária Sul.	
Data corrente: 	17/10/2022	
Data da última atualização: 	10/01/2023	
Autoria: 	PINHEIRO, N. B.; GASPAR, E. B.; MINHO, A. P.; DOMINGUES, R.; MOURA, M. Q. DE; VARELA JUNIOR, A. S.; CAPELLA, G. DE A.; STROTHMANN, A. L.; TERTO, W. D.; LEITE, F. P. L.	
Afiliação: 	NATÁLIA BERNE PINHEIRO, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; EMANUELLE BALDO GASPAR, CPPSUL; ALESSANDRO PELEGRINE MINHO, CPPSE; ROBERT DOMINGUES, CPPSUL; MICAELE QUINTANA DE MOURA, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; ANTÔNIO SERGIO VARELA JUNIOR, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; GABRIELA DE ALMEIDA CAPELLA, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; ADRIANE LEITES STROTHMANN, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; WESLEY DOUGLAS TERTO, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil; FABIO PEREIRA LEIVAS LEITE, Federal University of Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil.	
Título: 	Sheep immune-stimulated with Saccharomyces boulardii show reduced prolificacy of Haemonchus contortus.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Parasite Immunology, v. 44, n. 12, e12954, Dec. 2022.	
Páginas: 	6 p.	
DOI: 	10.1111/pim.12954	
Idioma: 	Inglês	
Conteúdo: 	Haemonchus contortus is the most pathogenic parasite for sheep. The objective was to evaluate immunomodulation of the probiotic Saccharomyces boulardii in sheep experimentally infected with H. contortus. Twenty-four sheep were divided into three groups: one infected with 500 H. contortus larvae/day for 26 days and supplemented with S. boulardii (40 ml with 1 × 108 CFU/ml/day); a control group only infected with H. contortus but not supplemented; and a naïve group that never came into contact with either parasites or S. boulardii. To assess the humoral immune response, production of specific serum IgG anti-somatic H. contortus antigen was evaluated through indirect ELISA. To assess the cellular immune response, cell populations and cytokine (IL-4, IL-5 and IL-10) production were evaluated through flow cytometry. For parasitological analyses, the counts of eggs per gram of faeces (EPG) and larvae per faecal culture were assessed. At all the study points, the concentration of IgG anti-H. contortus was higher (p < .05) in the S. boulardii group than in the other groups. The cell analysis revealed that there were significantly higher numbers (p < .05) of cells expressing MHC-II and significantly higher numbers (p < .05) of eosinophils in the mucosa in the S. boulardii group. Significant expression of IL-10 was observed only in the control infected group. There were significant reductions (p < .05) in EPG and larval counts in the S. boulardii supplemented group. These results show that S. boulardii supplementation modulated the immune response against H. contortus, thereby reducing its infection.	
Palavras-Chave: 	Nematodes.	
Thesagro: 	Controle Biológico; Fermento; Helminto; Nematóide; Ruminante.	
Thesaurus Nal: 	Biological control; Helminths; Immune system; Probiotics; Ruminants; Yeasts.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Pecuária Sul (CPPSUL)	
Exemplares: 	CPPSE - (25762-1) - UPC - AP - DD - PROCI-2022.00101 - PIN - 2022.00176 - DIS
CPPSUL - (14927-1) - UPC - AP - DD -  -  -  - DIS