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Registros recuperados : 277 | |
143. | | DODE, M. A. N.; JARDIM, P. O. da C. OSORIO, J. C. da S.; LUDER, W. E.; MACHADO, A. A. Estimativas dos principais cortes e da porcao comestivel da carcaca, em novilhos Holandes PB. Pesquisa Agropecuaria Brasileira, Brasilia, v.21, n.7, p.771-776, jul. 1986. Biblioteca(s): Embrapa Unidades Centrais. |
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146. | | FARIA, O. A. C.; CAIXETA, F. M.; DIAS, L. R. O.; SPRÍCIGO, J. F. W.; DODE, M. A. N. Effect of the anticipation of intrafollicular transfer of immature oocytes (IFIOT) in the nuclear maturation of bovine oocyte. Animal Reproduction, v. 16, n. 3, p. 587, Jul./Sept. 2019. Edição dos Proceedings of the 33nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Ilha da Comandatuba, BA, Brazil, August 15th to 19th, 2019. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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147. | | DIAS, L. R. O.; LEME, L. O.; SPRÍCIGO, J. F. W.; PIVATO, I.; DODE, M. A. N. Effect of delipidant agents during in vitro culture on the development, lipid content, gene expression, and cryotolerance of bovine embryos. Reproduction in Domestic Animals, v. 55, p. 11-20, 2020. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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150. | | GUIMARÃES, A. L. S.; CARVALHO, J. O.; LEME, L. O.; SPRÍCIGO, J. F. W.; PIVATO, I.; DODE, M. A. N. Efeito do cultivo in vitro no tamanho e sexo de embriões bovinos no D14 de desenvolvimento. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 29., 2015, Gramado. Anais... [S.l.]: SBTE, 2015. p. 337. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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151. | | DRIESSEN, K.; CARVALHO, J. O.; COSTA, M. P.; DODE, M. A. N.; MARTINS, C. F.; RUMPF, R. Avaliação estrutural e funcional de espermatozóides bovinos coletados a partir de epididímos resfriados por 24, 48 e 72 horas pós-morte animal. Acta Scientiae Veterinariae, Porto Alegre, v. 35, supl. 3, p. S954, 2007. Edição dos resumos do XXI Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Salvador, BA, ago. 2007. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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153. | | CAIXETA, E. S.; LEME, L. O.; MACHADO, G. M.; RUMPF, T.; FRANCO, M. M.; DODE, M. A. N. Competência ovocitária de acordo com o tamanho folicular: resultados parciais. Acta Scientiae Veterinariae, Porto Alegre, v. 35, supl. 3, p. S1156, 2007. Edição dos resumos do XXI Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Salvador, BA, ago. 2007. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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154. | | PIVATO, I.; SOUSA, R. V.; PEREIRA, D. C.; DESCHAMPS, J. C.; DODE, M. A. N.; RUMPF, R. Influência de diferentes tratamentos hormonais em doadoras de ovócitos na relação macho:fêmea de embriões bovinos produzidos in vitro. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 96. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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155. | | MACHADO, G. M.; LAENDER, C. R.; FRANCO, M. M.; LEME, L. O.; RUMPF, R.; DODE, M. A. N. Post-hatching bovine embryo development in vitro: relationship between sex and size. Reproduction, Fertility and Development, v. 20, n. 1, p. 146, 2008. Edição dos proceedings of the Annual Conference of the International, Embryo Society, Denver, Colorado, 2008. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 277 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/02/2013 |
Data da última atualização: |
07/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MACHADO, G. M.; CAIXETA, E. S.; LUCCI, C. M.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Zygote, v. 20, p. 123-134, 2011. |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. MenosThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demon... Mostrar Tudo |
Palavras-Chave: |
Cultura do embrião; Sexagem. |
Thesagro: |
Gado. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179338/1/posthatching-development-of-bovine-embryos-in-vitro-the-effects-of-tunnel-preparation-and-gender.pdf
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Marc: |
LEADER 02172naa a2200217 a 4500 001 1950734 005 2023-03-07 008 2011 bl uuuu u00u1 u #d 100 1 $aMACHADO, G. M. 245 $aPost-hatching development of bovine embryos in vitro$bthe effects of tunnel preparation and gender.$h[electronic resource] 260 $c2011 520 $aThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. 650 $aGado 653 $aCultura do embrião 653 $aSexagem 700 1 $aCAIXETA, E. S. 700 1 $aLUCCI, C. M. 700 1 $aRUMPF, R. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tZygote$gv. 20, p. 123-134, 2011.
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