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| Registros recuperados : 277 | |
| 162. |  | FIDELIS, A. A.; MELO, F. R.; MULINARI, F.; LIMA, F. W. O.; HODECKER, B. E. R.; DODE, M. A. N. Extracts from cerrado plants as antioxidant agents on in vitro embryo production. Animal Reprodroduction, v. 14, n. 3, p. 756, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 165. | | CARVALHO, B. P.; CUNHA, A. T. M.; SILVA, B. D. M.; SOUSA, R. V. de; LEME, L. O.; DODE, M. A. N.; MELO, E. de O. Production of transgenic cattle by somatic cell nuclear transfer (SCNT) with the human granulocyte colony-stimulation factor (hG-CSF). Journal of Animal Science and Technology, v. 61, n. 2, p. 61-68, 2019. Na publicação: Regivaldo V. Sousa, Eduardo O. Melo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 166. | | MACHADO, G. M.; FERREIRA, A. R.; PIVATO, I.; FIDELIS, A.; SPRICIGO, J. F.; PAULINI, F.; LUCCI, C. M.; FRANCO, M. M.; DODE, M. A. N. Post-hatching development of In vitro bovine embryos from day 7 to 14 in vivo versus in vitro. Molecular Reproduction & Development, v. 80, p. 936-947, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 167. | | ZACARIAS, T. A.; GUIMARÃES, A. L. S.; SCALIANTE, J. R.; RODRIGUES, S. A. D.; DODE, M. A. N.; FIGUEIREDO, R. A. Hormonal stimulation in Nelore calves for in vitro embryo production. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY (SBTE), 29., 2015, Gramado.; MEETING OF THE EUROPEAN EMBRYO TRANSFER ASSOCIATION (AETE), 31, 2015, Ghent. Proceedings... Animal Reproduction., v.12, n.3, p. 546, Jul./Sept. 2015. A172. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 168. | | CARVALHO, J. O.; MACHADO, G. M.; SIQUEIRA FILHO, E.; CAIXETA, E. S.; FRANCO, M. M.; RUMPF, R.; DODE, M. A. N. Gradiente de Percoll em diferentes volumes, tempo e força de centrifugação na produção In vitro e no sexo de embriões bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S514, 2008 Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 169. | | DODE, M. A. N.; CAIXETA, F. M. C.; VARGAS, L. N.; LEME, L. O.; KAWAMOTO, T. S.; FIDELIS, A. A. G.; FRANCO, M. M. Genome transfer technique for bovine embryo production using the metaphase plate and polar body. Journal of Assisted Reproduction and Genetics, v. 40, p. 943-951, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 170. | | PONTELO, T. P.; RODRIGUES, S. A. D.; KAWAMOTO, T. S.; LEME, L. O.; GOMES, A. C. M. M.; ZANGERONIMO, M. G.; FRANCO, M. M.; DODE, M. A. N. Histone acetylation during the in vitro maturation of bovine oocytes with different levels of competence. Reproduction, Fertility and Development, v. 32, n. 7, p. 690-696, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 171. | | PONTELO, T. P.; FRANCO, M. M.; KAWAMOTO, T. S.; CAIXETA, F. M. C.; LEME, L. de O.; KUSSANO, N. R.; ZANGERONIMO, M. G.; DODE, M. A. N. Histone deacetylase inhibitor during in vitro maturation decreases developmental capacity of bovine oocytes. PLoS One, v. 16, n. 3, e0247518, 2021.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 172. | | NOGUEIRA, E.; SANCHES, C.; COSTA E SILVA, E. V.; MENDES, A.; DODE, M. A. N.; WILEY, G.; KERNS, K.; SUTOVSKY, P. Identification of seminal parameters predictive of conception rates in Bos indicus cows submitted to timed-artificial insemination-partial results. Animal Reproduction, v. 15, n. 3, p. 463, jul./sept. 2018. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Biblioteca(s): Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia. |
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| 173. | | CAIXETA, F. M. C.; SOUSA, R. V.; GUIMARÃES, A. L.; LEME, L. O.; SPRÍCIGO, J. F. W.; SENNA NETTO, S. B.; PIVATO, I.; DODE, M. A. N. Meiotic arrest as an alternative to increase the production of bovine embryos by somatic cell nuclear transfer. Zygote, v. 25, p. 32-40, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 174. | | CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. Molecular Reproduction e Development, v. 79, p. 77-84, 2012.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 175. | | FAGUNDES, N. S.; MICHALCZECHEN-LACERDA, V. A.; CAIXETA, E. S.; MACHADO, G. M.; RODRIGUES, F. C.; MELO, E. de O.; DODE, M. A. N.; FRANCO, M. M. Methylation status in the intragenic differentially methylated region of the IGF2 locus in Bos taurus indicus oocytes with different developmental competencies. Molecular Human Reproduction, v. 17, n. 2, p. 85-91, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 176. | | ZACARIAS, T. A.; GUIMARÃES, A. L. S.; SCALIANTE, J. R.; RODRIGUES, S. de A. D.; DODE, M. A. N.; FIGUEIREDO, R. A. Manipulação hormonal em bezerras Nelore visando a produção in vitro de embriões. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 29., 2015, Gramado. Anais... [S.l.]: SBTE, 2015. p. 348.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 177. | | MARTINS, C. F.; FELICIANO SILVA, A. E. D.; DODE, M. A. N.; RUMPF, R.; CUMPA, H. C. B.; SILVA, C. G.; PIVATO, I. Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules. Cryobiology, v. 71, p. 47-53, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 178. | | MARTINS, C. F.; FELICIANO SILVA, A. E. D.; DODE, M. A. N.; RUMPF, R.; CUMPA, H. C. B.; SILVA, C. G.; PIVATO, I. Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules. Cryobiology, v. 71, p. 47-53, 2015. p. 47-53| Biblioteca(s): Embrapa Cerrados. |
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| 179. | | ANDRADE, E. R.; RODRIGUES, A. P. R.; AMORIM, C. A.; CARVALHO, F. C. A.; DODE, M. A. N.; FIGUEIREDO, J. R. Short term maintenance of sheep preantral follicles in situ in 0.9% saline and Braun-Collins solution. Small Ruminant Research, v.41, n.2, p.141-149, 2001.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 277 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
JOSE O. CARVALHO, UNIVERSIDADE DE SÃO PAULO; VALQUIRIA A. MICHALCZECHEN-LACERDA, UnB; ROBERTO SARTORI, UNIVERSIDADE DE SÃO PAULO; FERNANDA C. RODRIGUES; OTAVIO BRAVIM, UnB; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. |
|
Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Reproduction e Development, v. 79, p. 77-84, 2012. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed. |
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Palavras-Chave: |
Gene IGF2; Gene IGF2R; Sexagem por citometria. |
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Thesagro: |
Bovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179337/1/Carvalho-et-al-2012-Molecular-Reproduction-and-Development.pdf
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|
Marc: |
LEADER 02074nam a2200229 a 4500
001 1950725
005 2023-03-07
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. O.
245 $aThe methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.$h[electronic resource]
260 $aMolecular Reproduction e Development, v. 79, p. 77-84$c2012
520 $aThe objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed.
650 $aBovino
653 $aGene IGF2
653 $aGene IGF2R
653 $aSexagem por citometria
700 1 $aMICHALCZECHEN-LACERDA, V. A.
700 1 $aSARTORI, R.
700 1 $aRODRIGUES, F. C.
700 1 $aBRAVIM, O.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
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