Data/hora: 23/06/2024 19:52:56

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Pecuária Sudeste.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 1	
Data corrente: 	27/05/2022	
Data da última atualização: 	20/06/2022	
Autoria: 	SOUZA, M. M. de; NICIURA, S. C. M.; ROCHA, M. I. P.; PAN, Z.; ZHOU, H.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; AFONSO, J.; OLIVEIRA, P. S. N. de; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L.; KOLTES, J. E.; REGITANO, L. C. de A.	
Afiliação: 	MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; SIMONE CRISTINA MEO NICIURA, CPPSE; MARINA IBELLI PEREIRA ROCHA, UFSCAR; ZHANGYUAN PAN, UNIVERSITY OF CALIFORNIA; HUAIJUN ZHOU, UNIVERSITY OF CALIFORNIA; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; JULIANA AFONSO; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; GERSON B. MOURÃO, ESALQ/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; JAMES E. KOLTES, IOWA STATE UNIVERSITY; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.	
Título: 	DNA methylation may affect beef tenderness through signal transduction in Bos indicus.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Epigenetics & Chromatin, v. 15, n. 1, p. 1-16, 2022.	
DOI: 	https://doi.org/10.1186/s13072-022-00449-4	
Idioma: 	Inglês	
Notas: 	Article number: 15.	
Conteúdo: 	Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation.	
Palavras-Chave: 	EBF3; Epigenoma; Epigenome; Força de cisalhamento; GNAS; Muscle; Músculo de nelore; Nelore; RRBS; Shear force.	
Thesagro: 	Bos Indicus; Bos Taurus; Metilação; Músculo.	
Thesaurus Nal: 	Cattle; DNA methylation; Methylation.	
Categoria do assunto: 	--
X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144144/1/DNA-MethylationMay.pdf	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CNPTIA - (21212-1) - UPC - AP - DD -  -  -  - DIS
CPPSE - (25654-1) - UPC - AP - DD - PROCI-2022.00028 - SOU - 2022.00077 - DIS