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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
31/03/1993 |
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Data da última atualização: |
05/03/2024 |
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Autoria: |
RESENDE, R. de O.; HAAN, P. de; VOSSEN, E. van de; AVILA, A. C. de; GOLDBACH, R.; PETERS, D. |
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Afiliação: |
RENATO DE OLIVEIRA RESENDE; PETER DE HAAN; EDWIN VAN DE VOSSEN; ANTONIO CARLOS DE AVILA, CNPH; ROB GOLDBACH; DICK PETERS. |
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Título: |
Defective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of General Virology, v. 73, p. 2509-2516, 1992. |
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DOI: |
https://doi.org/10.1099/0022-1317-73-10-2509 |
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Idioma: |
Inglês |
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Conteúdo: |
Defective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment. MenosDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another conta... Mostrar Tudo |
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Palavras-Chave: |
Caracterizacao; Characterization; DI L RNA; Generation; Geracao; Maintenance; TSWV; Vira-Cabeca. |
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Thesagro: |
Manutenção. |
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Thesaurus Nal: |
Tomato spotted wilt virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02548naa a2200313 a 4500
001 1749565
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1099/0022-1317-73-10-2509$2DOI
100 1 $aRESENDE, R. de O.
245 $aDefective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions.$h[electronic resource]
260 $c1992
520 $aDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment.
650 $aTomato spotted wilt virus
650 $aManutenção
653 $aCaracterizacao
653 $aCharacterization
653 $aDI L RNA
653 $aGeneration
653 $aGeracao
653 $aMaintenance
653 $aTSWV
653 $aVira-Cabeca
700 1 $aHAAN, P. de
700 1 $aVOSSEN, E. van de
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R.
700 1 $aPETERS, D.
773 $tJournal of General Virology$gv. 73, p. 2509-2516, 1992.
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Embrapa Hortaliças (CNPH) |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
20/10/2020 |
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Data da última atualização: |
20/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BAMPI, D.; BORSTNEZ, K. K.; DIAS, C. P.; DALLA COSTA, O. A.; MOREIRA, F.; OLIVEIRA JÚNIOR, J. M.; SCHWEGLER, E.; RAUBER, L. P.; BIANCHI, I. |
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Afiliação: |
IFC/Araquari; IFC/Araquari; UEL; OSMAR ANTONIO DALLA COSTA, CNPSA; IFC/Araquari; IFC/Araquari; IFC/Araquari; IFC/Concórdia; IFC/Araquari. |
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Título: |
Evaluation of reproductive and animal welfare parameters of swine females of different genetic lines submitted to different reproductive management and housing systems during pregnancy. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 72, n. 5, p.1675-1682, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this study was to evaluate swine females of different genetic lines submitted to different reproductive management and housing systems during pregnancy on reproductive performance and animal welfare parameters. After artificial insemination protocol, 524 females were divided into two gestation housing systems: PEN1=animals housed in individual stalls during the breeding and after group-housed; PEN32=animals housed in individual stalls from breeding until 32 days of pregnancy and after group-housed. The number of piglets born, and the pregnancy and farrowing rates were evaluated. Welfare parameters related to the pregnancy phase were used. Females who weaned more piglets in the previous farrowing had a higher number of piglets born at the next farrowing. The pregnancy rate was affected by the number of semen doses. The farrowing rate was not influenced by the evaluated parameters, with average value of 91.36%. There was no effect of the gestation housing system and the genetic lines on pregnancy and farrowing rates, with values above 90.0%. The animal welfare indicators showed more compromised parameters in PEN1 system. PEN1 system did not impair the reproductive performance although it presented more compromised animal welfare parameters. |
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Palavras-Chave: |
Alojamento coletivo; Baias individuais; Bem-estar animal; Fêmea suína. |
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Thesagro: |
Período de Gestação; Porca; Suinocultura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216863/1/final9523.pdf
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Marc: |
LEADER 02269naa a2200301 a 4500
001 2125705
005 2020-10-20
008 2020 bl uuuu u00u1 u #d
100 1 $aBAMPI, D.
245 $aEvaluation of reproductive and animal welfare parameters of swine females of different genetic lines submitted to different reproductive management and housing systems during pregnancy.$h[electronic resource]
260 $c2020
520 $aAbstract: The aim of this study was to evaluate swine females of different genetic lines submitted to different reproductive management and housing systems during pregnancy on reproductive performance and animal welfare parameters. After artificial insemination protocol, 524 females were divided into two gestation housing systems: PEN1=animals housed in individual stalls during the breeding and after group-housed; PEN32=animals housed in individual stalls from breeding until 32 days of pregnancy and after group-housed. The number of piglets born, and the pregnancy and farrowing rates were evaluated. Welfare parameters related to the pregnancy phase were used. Females who weaned more piglets in the previous farrowing had a higher number of piglets born at the next farrowing. The pregnancy rate was affected by the number of semen doses. The farrowing rate was not influenced by the evaluated parameters, with average value of 91.36%. There was no effect of the gestation housing system and the genetic lines on pregnancy and farrowing rates, with values above 90.0%. The animal welfare indicators showed more compromised parameters in PEN1 system. PEN1 system did not impair the reproductive performance although it presented more compromised animal welfare parameters.
650 $aPeríodo de Gestação
650 $aPorca
650 $aSuinocultura
653 $aAlojamento coletivo
653 $aBaias individuais
653 $aBem-estar animal
653 $aFêmea suína
700 1 $aBORSTNEZ, K. K.
700 1 $aDIAS, C. P.
700 1 $aDALLA COSTA, O. A.
700 1 $aMOREIRA, F.
700 1 $aOLIVEIRA JÚNIOR, J. M.
700 1 $aSCHWEGLER, E.
700 1 $aRAUBER, L. P.
700 1 $aBIANCHI, I.
773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia$gv. 72, n. 5, p.1675-1682, 2020.
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