| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
21/06/2001 |
|
Data da última atualização: |
21/06/2001 |
|
Autoria: |
JAMES, E. K.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JÚNIOR, F. B. dos; SILVA, L. G. da; REIS, V. M. |
|
Título: |
Further observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. |
|
Ano de publicação: |
2001 |
|
Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 52, n. 357, p. 747-760, apr. 2001. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Sugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody. MenosSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem p... Mostrar Tudo |
|
Palavras-Chave: |
Bactéria endofítica; Biological nitrogen fixation; BNF; Cana-de-açúcar; Casa de vegetação; Endophytic bacteria; FBN; Fixação bológica de nitrogênio; Greenhouse; Saccharum spp. |
|
Thesaurus Nal: |
Gluconacetobacter diazotrophicus; sugarcane. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03060naa a2200325 a 4500
001 1598609
005 2001-06-21
008 2001 bl --- 0-- u #d
100 1 $aJAMES, E. K.
245 $aFurther observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions.
260 $c2001
520 $aSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody.
650 $aGluconacetobacter diazotrophicus
650 $asugarcane
653 $aBactéria endofítica
653 $aBiological nitrogen fixation
653 $aBNF
653 $aCana-de-açúcar
653 $aCasa de vegetação
653 $aEndophytic bacteria
653 $aFBN
653 $aFixação bológica de nitrogênio
653 $aGreenhouse
653 $aSaccharum spp
700 1 $aOLIVARES, F. L.
700 1 $aOLIVEIRA, A. L. M. de
700 1 $aREIS JÚNIOR, F. B. dos
700 1 $aSILVA, L. G. da
700 1 $aREIS, V. M.
773 $tJournal of Experimental Botany, London$gv. 52, n. 357, p. 747-760, apr. 2001.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio Ambiente; Embrapa Pecuária Sudeste. |
|
Data corrente: |
12/11/2012 |
|
Data da última atualização: |
27/06/2023 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
SOLORZANO, L. A. R.; BERNDT, A.; FRIGHETTO, R. T. S.; PERNA JÚNIOR, F.; DEMARCHI, J. J. A. de A.; RODRIGUES, P. H. M. |
|
Afiliação: |
LAURA ALEXANDRA ROMERO SOLÓRZANO, MESTRANDA EM NUTRIÇÃO E PRODUÇÃO ANIMAL DA FMVZ/USP/ PIRASSUNUNGA; ALEXANDRE BERNDT, CPPSE; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; FLAVIO PERNA JUNIOR, MESTRANDO EM NUTRIÇÃO E PRODUÇÃO ANIMAL DA FMVZ/USP/PIRASSUNUNGA; JOAO JOSÉ ASSUMPÇÃO DE ABREU DEMARCHI, APTA/NOVA ODESSA; PAULO HENRIQUE MAZZA RODRIGUES, PROF. FMVZ/USP/PIRASSUNUNGA. |
|
Título: |
Efeito de três fontes energéticas sobre a produção de metano em bovinos. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília, DF. A produção animal no mundo em transformação: anais. Brasília: SBZ, 2012. |
|
Descrição Física: |
1 CD-ROM |
|
Idioma: |
Português |
|
Conteúdo: |
Resumo: O objetivo deste trabalho foi avaliar o efeito de três fontes energéticas sobre a produção de metano em bovinos utilizando-se a técnica do gás traçador hexafluoreto de enxofre (SF6). Foram utilizadas seis vacas (730 ± 70 kg) canuladas no rúmen, distribuídas a três dietas, seguindo-se delineamento experimental em quadrado latino 3x3 replicado (n= 18 unidades experimentais): Controle (CON); Soja (SOJ): dieta de alto teor de extrato etéreo (inclusão de 15% de soja) e Polpa cítrica (POL): dieta de baixo teor de extrato etéreo e alta participação de pectina (inclusão de 15% de polpa). Cada período experimental foi constituído de 21 dias, sendo 15 dias para adaptação e os últimos 6 dias destinados para coleta de dados, para determinação do consumo de matéria seca e a produção de metano (CH4). As concentrações de CH4 e SF6 foram determinadas por cromatografia gasosa. Não houve diferenças significativas (P<0,10) entre os tratamentos para o consumo de MS expresso em quilograma por dia (kg/dia) ou porcentagem do peso vivo (% PV). A emissão de CH4 pelos bovinos foi de 286,22 a 344,22 g/d; 103,71 a 125,64 kg/ano; 17,41 a 22,03 g/kg MSI; 5,17 a 6,58% de EB perdida na forma de metano e 3,77 a 4,53 Mcal/Ani/d. As médias da produção de metano em g/d; kg/ano e Mcal/Ani/d, não foram diferentes entre os tratamentos à 5% de probabilidade. Abstract: The objective of this study was to evaluate the effect of three energy sources on methane production in cattle using the tracer technique sulfur hexafluoride (SF6). It were used six cows (730 ± 70 kg) cannulated in the rumen, distributed into three experimental treatments, followed by experimental design replicated 3x3 Latin square (n = 18 plots): Control (CON), Soybean (SOJ): high ether extract content diet (including 15% soy) and Citrus Pulp (POL): diet low in lipids, and high participation of pectin (including 15% of pulp). Each experimental period consisted of 21 days and 15 days for adaptation and the last six days for data collection for determination of dry matter intake and methane (CH4). The concentrations of SF6 and CH4 were determined by gas chromatography. There were no significant differences (P <0.10) among treatments for DMI expressed as kilograms per day (kg/day) and percentage of body weight (% BW). The CH4 emission by cattle was 286.22 to 344.22 g/d; 103.71 to 125.64 kg/year, from 17.41 to 22.03 g/kg MSI, 5.17 to 6.58% GE lost in the form of methane and 3.77 to 4.53 Mcal/Ani/d. The average methane production in g/d; kg/year and Mcal/Ani/d were not different between treatments at 5% probability. MenosResumo: O objetivo deste trabalho foi avaliar o efeito de três fontes energéticas sobre a produção de metano em bovinos utilizando-se a técnica do gás traçador hexafluoreto de enxofre (SF6). Foram utilizadas seis vacas (730 ± 70 kg) canuladas no rúmen, distribuídas a três dietas, seguindo-se delineamento experimental em quadrado latino 3x3 replicado (n= 18 unidades experimentais): Controle (CON); Soja (SOJ): dieta de alto teor de extrato etéreo (inclusão de 15% de soja) e Polpa cítrica (POL): dieta de baixo teor de extrato etéreo e alta participação de pectina (inclusão de 15% de polpa). Cada período experimental foi constituído de 21 dias, sendo 15 dias para adaptação e os últimos 6 dias destinados para coleta de dados, para determinação do consumo de matéria seca e a produção de metano (CH4). As concentrações de CH4 e SF6 foram determinadas por cromatografia gasosa. Não houve diferenças significativas (P<0,10) entre os tratamentos para o consumo de MS expresso em quilograma por dia (kg/dia) ou porcentagem do peso vivo (% PV). A emissão de CH4 pelos bovinos foi de 286,22 a 344,22 g/d; 103,71 a 125,64 kg/ano; 17,41 a 22,03 g/kg MSI; 5,17 a 6,58% de EB perdida na forma de metano e 3,77 a 4,53 Mcal/Ani/d. As médias da produção de metano em g/d; kg/ano e Mcal/Ani/d, não foram diferentes entre os tratamentos à 5% de probabilidade. Abstract: The objective of this study was to evaluate the effect of three energy sources on methane production in cattle using the tracer technique su... Mostrar Tudo |
|
Palavras-Chave: |
Gases de Efeito Estufa; Grãos de Soja; Lipídeos; Polpa Cítrica; Ruminantes. |
|
Thesagro: |
Bovino; Efeito estufa; Metano; Soja. |
|
Thesaurus NAL: |
Cattle; Methane production; Soybeans. |
|
Categoria do assunto: |
--
S Ciências Biológicas |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/69923/1/PROCI-2012.00146.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/74422/1/2012AA036.pdf
|
|
Marc: |
LEADER 03633nam a2200325 a 4500
001 1939451
005 2023-06-27
008 2012 bl uuuu u00u1 u #d
100 1 $aSOLORZANO, L. A. R.
245 $aEfeito de três fontes energéticas sobre a produção de metano em bovinos.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília, DF. A produção animal no mundo em transformação: anais. Brasília: SBZ$c2012
300 $c1 CD-ROM
520 $aResumo: O objetivo deste trabalho foi avaliar o efeito de três fontes energéticas sobre a produção de metano em bovinos utilizando-se a técnica do gás traçador hexafluoreto de enxofre (SF6). Foram utilizadas seis vacas (730 ± 70 kg) canuladas no rúmen, distribuídas a três dietas, seguindo-se delineamento experimental em quadrado latino 3x3 replicado (n= 18 unidades experimentais): Controle (CON); Soja (SOJ): dieta de alto teor de extrato etéreo (inclusão de 15% de soja) e Polpa cítrica (POL): dieta de baixo teor de extrato etéreo e alta participação de pectina (inclusão de 15% de polpa). Cada período experimental foi constituído de 21 dias, sendo 15 dias para adaptação e os últimos 6 dias destinados para coleta de dados, para determinação do consumo de matéria seca e a produção de metano (CH4). As concentrações de CH4 e SF6 foram determinadas por cromatografia gasosa. Não houve diferenças significativas (P<0,10) entre os tratamentos para o consumo de MS expresso em quilograma por dia (kg/dia) ou porcentagem do peso vivo (% PV). A emissão de CH4 pelos bovinos foi de 286,22 a 344,22 g/d; 103,71 a 125,64 kg/ano; 17,41 a 22,03 g/kg MSI; 5,17 a 6,58% de EB perdida na forma de metano e 3,77 a 4,53 Mcal/Ani/d. As médias da produção de metano em g/d; kg/ano e Mcal/Ani/d, não foram diferentes entre os tratamentos à 5% de probabilidade. Abstract: The objective of this study was to evaluate the effect of three energy sources on methane production in cattle using the tracer technique sulfur hexafluoride (SF6). It were used six cows (730 ± 70 kg) cannulated in the rumen, distributed into three experimental treatments, followed by experimental design replicated 3x3 Latin square (n = 18 plots): Control (CON), Soybean (SOJ): high ether extract content diet (including 15% soy) and Citrus Pulp (POL): diet low in lipids, and high participation of pectin (including 15% of pulp). Each experimental period consisted of 21 days and 15 days for adaptation and the last six days for data collection for determination of dry matter intake and methane (CH4). The concentrations of SF6 and CH4 were determined by gas chromatography. There were no significant differences (P <0.10) among treatments for DMI expressed as kilograms per day (kg/day) and percentage of body weight (% BW). The CH4 emission by cattle was 286.22 to 344.22 g/d; 103.71 to 125.64 kg/year, from 17.41 to 22.03 g/kg MSI, 5.17 to 6.58% GE lost in the form of methane and 3.77 to 4.53 Mcal/Ani/d. The average methane production in g/d; kg/year and Mcal/Ani/d were not different between treatments at 5% probability.
650 $aCattle
650 $aMethane production
650 $aSoybeans
650 $aBovino
650 $aEfeito estufa
650 $aMetano
650 $aSoja
653 $aGases de Efeito Estufa
653 $aGrãos de Soja
653 $aLipídeos
653 $aPolpa Cítrica
653 $aRuminantes
700 1 $aBERNDT, A.
700 1 $aFRIGHETTO, R. T. S.
700 1 $aPERNA JÚNIOR, F.
700 1 $aDEMARCHI, J. J. A. de A.
700 1 $aRODRIGUES, P. H. M.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
