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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/01/2011 |
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Data da última atualização: |
15/10/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JONSSON, C. M.; AOYAMA, H. |
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Afiliação: |
CLAUDIO MARTIN JONSSON, CNPMA; Hiroshi Aoyama, Instituto de Biologia - UNICAMP. |
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Título: |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
BioMetals, Oxford, v. 23, p. 93-98, 2010. |
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DOI: |
10.1007/s10534-009-9270-z |
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Idioma: |
Inglês |
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Conteúdo: |
The presence of copper in water environment may have detrimental effects on aquatic organisms, including algae, where different enzymatic systems can be affected. Algae acid phosphatase plays important roles in metabolic processes such as decomposition of organic phosphate, autophagic digestive process, recycling cellular materials and zygote formation during reproduction. This work describes an in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirchneriella subcapitata (formely Selenastrum capricornutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol-1 were determined in the absence and in the presence of 0.2 mM Cu2+, respectively. The dissociation constant value for Cu2+ binding to the enzyme was determined to be 22.04 uM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2+ correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples. |
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Thesagro: |
Fosfatase Ácida. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01625naa a2200157 a 4500
001 1872588
005 2015-10-15
008 2010 bl --- 0-- u #d
024 7 $a10.1007/s10534-009-9270-z$2DOI
100 1 $aJONSSON, C. M.
245 $aEffect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata.
260 $c2010
520 $aThe presence of copper in water environment may have detrimental effects on aquatic organisms, including algae, where different enzymatic systems can be affected. Algae acid phosphatase plays important roles in metabolic processes such as decomposition of organic phosphate, autophagic digestive process, recycling cellular materials and zygote formation during reproduction. This work describes an in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirchneriella subcapitata (formely Selenastrum capricornutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol-1 were determined in the absence and in the presence of 0.2 mM Cu2+, respectively. The dissociation constant value for Cu2+ binding to the enzyme was determined to be 22.04 uM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2+ correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples.
650 $aFosfatase Ácida
700 1 $aAOYAMA, H.
773 $tBioMetals, Oxford$gv. 23, p. 93-98, 2010.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
22/12/2005 |
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Data da última atualização: |
22/12/2005 |
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Autoria: |
JESUS, D. F.; LEITE, M. F.; DELLA MODESTA, R. C.; MATTA, V. M.; CABRAL, L. M. C. |
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Título: |
Avaliação sensorial de suco de laranja concentrado por osmose inversa. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In.: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 5., 2003, Campinas, SP. Resumos... Campinas, 2003. 1 CD-Rom. |
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Idioma: |
Português |
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Palavras-Chave: |
Análise sensorial; Omose inversa. |
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Thesagro: |
Laranja; Suco Concentrado. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00646naa a2200205 a 4500
001 1416014
005 2005-12-22
008 2003 bl uuuu u00u1 u #d
100 1 $aJESUS, D. F.
245 $aAvaliação sensorial de suco de laranja concentrado por osmose inversa.
260 $c2003
650 $aLaranja
650 $aSuco Concentrado
653 $aAnálise sensorial
653 $aOmose inversa
700 1 $aLEITE, M. F.
700 1 $aDELLA MODESTA, R. C.
700 1 $aMATTA, V. M.
700 1 $aCABRAL, L. M. C.
773 $tIn.: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 5., 2003, Campinas, SP. Resumos... Campinas, 2003. 1 CD-Rom.
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