Data/hora: 09/06/2024 17:37:06

Biblioteca(s): | Embrapa Amazônia Oriental; Embrapa Instrumentação.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 08/02/2012
Data da última atualização: | 18/09/2018
Autoria: | DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S.
Afiliação: | CRISTIANE SANCHEZ FARINAS, CNPDIA.
Título: | Using amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes.
Ano de publicação: | 2012
Fonte/Imprenta: | Biomass and Bioenergy,Oxford, v. 37, p. 243-250, 2012.
DOI: | 10.1016/j.biombioe.2011.12.006
Idioma: | Inglês
Conteúdo: | The successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, b-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g1 for CMCase, 5.0 FPU g1 for FPAse, 105.82 IU g1 for b-glucosidase and 1055.62 IU g1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 C or pH 3e3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion.
Palavras-Chave: | Agro-industrial residues; Amazon forest; Floresta amazônica; Resíduo agroindustrial.
Thesagro: | Enzima; Fungo.
Thesaurus Nal: | Bioethanol; Cellulases; Enzymes; Solid state fermentation.

Categoria do assunto:

K Ciência Florestal e Produtos de Origem Vegetal
S Ciências Biológicas

Registro original: | Embrapa Instrumentação (CNPDIA)

Exemplares:

CPATU - (45505-1) - UPC - AP - DD -  -  -  - DIS
CNPDIA - (14130-1) - UPC - AP - DD - PROCI-12.00172 -  - 2012.00172 - DIS