Data/hora: 21/09/2024 10:07:55

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	05/03/2012	
Data da última atualização: 	19/01/2017	
Autoria: 	WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H.	
Afiliação: 	BEATRIZ WIEBKE-STROHM, UFRGS; GIANCARLO PASQUALI, UFRGS; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARTA BENCKE, UFRGS; LAURO BÜCKER NETO, UFRGS; ARLETE B. BECKER-RITT, UFRGS; ANNE H. S. MARTINELLI, UFRGS; CILIANA RECHENMACHER, UFRGS; JOSEPH C. POLACCO, University of Missouri; RENATA STOLF, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MILENA S. HOMRICH, UFRGS; EMERSON M. DEL PONTE, UFRGS; CELIA R. CARLINI, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSo; MARIA HELENA BODANESE-ZANETTINI, UFRGS.	
Título: 	Ubiquitous urease affects soybean susceptibility to fungi.	
Ano de publicação: 	2012	
Fonte/Imprenta: 	Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012.	
DOI: 	DOI 10.1007/s11103-012-9894-1	
Idioma: 	Inglês	
Conteúdo: 	The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach.	
Palavras-Chave: 	Penicillium herguei; Transformação genética.	
Thesagro: 	Fungo; Phomopsis; Queima da saia; Soja.	
Thesaurus Nal: 	Genetic transformation; Soybeans.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (32839-1) - UPC - AP - PP - 10135 -  - 10135 - DIS