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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
13/08/1999 |
Data da última atualização: |
13/08/1999 |
Autoria: |
CONNOLLY, A. G.; GODWIN, I. D.; COOPER, M.; DELACY, I. H. |
Afiliação: |
Department of Crop Science, University of Zimbabwe, P.O. Box MP147, Harare, Zimbabwe. |
Título: |
Interpretation of randomly amplified polymorphic DNA marker data for fingerprinting sweet potato (Ipomoea batatas L.) genotypes |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
Theor Appl Genet, v.88, p.332-336, 1994 |
Idioma: |
Inglês |
Conteúdo: |
In this paper we present a method for the generation of randomly amplified polymorphic DNA (RAPD) markers for sweet potato. These were applied to produce genetic fingerprints of six clonal cultivars and to estimate genetic distances between these cultivars. The level of polymorphism within the species was extremely high. From the 36-decamer random primers used, 170 fragments were amplified, of which 132 (77,6%) were polymorphic. Ten primers resulted in no detected amplification. Of the remaining 26 primers for which amplification was achieved, only only one did not reveal polymorphism. Six primers used alone enabled the discrimination of all six genotypes. Pattern analysis, which employed both a classification and ordination method, enabled the grouping of cultivara and the identification of primers which gave greatest discrimination among the cultivars. |
Palavras-Chave: |
Molecular markers; RAPDs; Sweet potato. |
Thesagro: |
Ipomoea Batatas. |
Thesaurus Nal: |
genetic polymorphism. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01499naa a2200217 a 4500 001 1648249 005 1999-08-13 008 1994 bl --- 0-- u #d 100 1 $aCONNOLLY, A. G. 245 $aInterpretation of randomly amplified polymorphic DNA marker data for fingerprinting sweet potato (Ipomoea batatas L.) genotypes 260 $c1994 520 $aIn this paper we present a method for the generation of randomly amplified polymorphic DNA (RAPD) markers for sweet potato. These were applied to produce genetic fingerprints of six clonal cultivars and to estimate genetic distances between these cultivars. The level of polymorphism within the species was extremely high. From the 36-decamer random primers used, 170 fragments were amplified, of which 132 (77,6%) were polymorphic. Ten primers resulted in no detected amplification. Of the remaining 26 primers for which amplification was achieved, only only one did not reveal polymorphism. Six primers used alone enabled the discrimination of all six genotypes. Pattern analysis, which employed both a classification and ordination method, enabled the grouping of cultivara and the identification of primers which gave greatest discrimination among the cultivars. 650 $agenetic polymorphism 650 $aIpomoea Batatas 653 $aMolecular markers 653 $aRAPDs 653 $aSweet potato 700 1 $aGODWIN, I. D. 700 1 $aCOOPER, M. 700 1 $aDELACY, I. H. 773 $tTheor Appl Genet$gv.88, p.332-336, 1994
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Embrapa Mandioca e Fruticultura (CNPMF) |
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46. | | DEL DUCA, L. de J. A.; GUARIENTI, E. M.; FONTANELI, R. S.; ZANOTTO, D. L. Influência de cortes simulando pastejo na composição química de grãos de cereais de inverno. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 9, p. 1607-1614, set. 1999.Biblioteca(s): Embrapa Trigo; Embrapa Unidades Centrais. |
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53. | | DEL DUCA, L. de J. A.; PRESTES, A. M.; SOUSA, C. N. A. de; GUARIENTI, E. M.; PHILIPOVSKY, J. F. Características agronômicas, fitossanitárias e industriais das cultivares e das linhagens em experimentação no Paraná em 1994. In: REUNIÃO DA COMISSÃO CENTRO-SUL BRASILEIRA DE PESQUISA DE TRIGO, 11., 1995, Cascavel. Ata... Cascavel: OCEPAR, 1995. p. 95-96Biblioteca(s): Embrapa Trigo. |
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