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Registros recuperados : 2 | |
1. | | VOORT, M. van der; MEIJER, H. J. G.; SCHMIDT, Y.; WATROUS, J; DEKKERS, E.; MENDES, R.; DORRESTEIN, P. C; GROSS, H.; RAAIJMAKERS, J. M. Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds. Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 696. 14 p. Biblioteca(s): Embrapa Meio Ambiente. |
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2. | | MENDES, R.; KRUIJT, M.; BRUIJN, I. de; DEKKERS, E.; VOORT, M. van der; SCHNEIDER, J. H. M.; PICENO, Y. M.; DESANTIS, T. Z.; ANDERSEN G. L.; BAKKER, P. A. H. M.; RAAIJMAKERS, J. M. Deciphering the rhizosphere microbiome for disease-suppressive bacteria. Science, Washington, DC, v. 332, n. 6033, p. 1097-1100, 2011. Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 2 | |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
04/01/2016 |
Data da última atualização: |
25/01/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VOORT, M. van der; MEIJER, H. J. G.; SCHMIDT, Y.; WATROUS, J; DEKKERS, E.; MENDES, R.; DORRESTEIN, P. C; GROSS, H.; RAAIJMAKERS, J. M. |
Afiliação: |
MENNO VAN DER VOORT, Wageningen University; HAROLD J G MEIJER, Wageningen University; YVONNE SCHMIDT, University of Bonn; JERAMIE WATROUS, University of California; ESTER DEKKERS, Wageningen University; RODRIGO MENDES, CNPMA; PIETER C DORRESTEIN, University of California; HARALD GROSS, University of California; JOS M RAAIJMAKERS, Wageningen University. |
Título: |
Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 696. |
Páginas: |
14 p. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results show an enormous metabolic potential for Pseudomonas sp. SH-C52, with at least three structurally diverse lipopeptides, each with a different antimicrobial activity spectrum. MenosAbstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results sh... Mostrar Tudo |
Palavras-Chave: |
Biocontrol; Genomes sequencing; Pseudomonads. |
Thesagro: |
Bactéria; Beterraba; Controle biológico; Peptídeo; Pseudomonas sp; Rizosfera. |
Thesaurus NAL: |
Antimicrobial peptides; Beneficial microorganisms; Biological control; Mass spectrometry; Rhizosphere bacteria; Sequence analysis. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136527/1/2015AP29.pdf
|
Marc: |
LEADER 02843naa a2200409 a 4500 001 2032807 005 2016-01-25 008 2015 bl uuuu u00u1 u #d 100 1 $aVOORT, M. van der 245 $aGenome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds.$h[electronic resource] 260 $c2015 300 $a14 p. 520 $aAbstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results show an enormous metabolic potential for Pseudomonas sp. SH-C52, with at least three structurally diverse lipopeptides, each with a different antimicrobial activity spectrum. 650 $aAntimicrobial peptides 650 $aBeneficial microorganisms 650 $aBiological control 650 $aMass spectrometry 650 $aRhizosphere bacteria 650 $aSequence analysis 650 $aBactéria 650 $aBeterraba 650 $aControle biológico 650 $aPeptídeo 650 $aPseudomonas sp 650 $aRizosfera 653 $aBiocontrol 653 $aGenomes sequencing 653 $aPseudomonads 700 1 $aMEIJER, H. J. G. 700 1 $aSCHMIDT, Y. 700 1 $aWATROUS, J 700 1 $aDEKKERS, E. 700 1 $aMENDES, R. 700 1 $aDORRESTEIN, P. C 700 1 $aGROSS, H. 700 1 $aRAAIJMAKERS, J. M. 773 $tFrontiers in Microbiology, Lausanne$gv. 6, 2015. Article 696.
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Embrapa Meio Ambiente (CNPMA) |
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