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| Registros recuperados : 21 | |
| 3. |  | CAITAR, V. S. L.; NASCIMENTO, L. C.; CARVALHO, M. C. D. C. G. D.; DARBEN, L. M; PODANOSQUI, A. M. P.; DIAS, W. P.; GUIMARÃES, F. C. M. Earlier transcriptome interaction from resistant and susceptible soybean genotypes under Pratylenchus brachyurus infection revealed by RNA-seq. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. [Anais...]. [Brasília]: Sociedade Brasileira de Fitopatologia, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 4. | | AOYAGI, L. N.; CAITAR, V. S. L.; POLIZEL-PODANOSQUI, A. M.; DARBEN, L. M.; CARVALHO, M. C. D.; KUWAHARA, M. K.; MARCELINO-GUIMARÃES, F. C. Identificação e caracterização filogenética de proteínas R2R3-MYB no genoma da soja e seu perfil transcricional em resposta a patógenos. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. [Anais...]. [Brasília]: Sociedade Brasileira de Fitopatologia, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 5. | | SOARES, R. M.; FANTINATO, G. G. P.; DARBEN, L. M.; MARCELINO-GUIMARÃES, F. C.; SEIXAS, C. D. S.; CARNEIRO, G. E. de S. First report of Curtobacterium flaccumfaciens pv. flaccumfaciens on soybean in Brazil. Tropical Plant Pathology, Brasília, DF, v. 38, n. 5, p. 452-454, Sept./Oct. 2013.| Biblioteca(s): Embrapa Soja. |
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| 6. | | DARBEN, L. M.; QI, M.; YOKOYAMA, A.; CARVALHO, M. C. C. G. de; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Functional characterization of P. pachyrhizi effector candidates. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 840.pdf.| Biblioteca(s): Embrapa Soja. |
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| 7. | | CARVALHO, K. de; RINCÃO, M. P.; DARBEN, L. M.; CARVALHO, M. C. C. G. de; LOPES, I. de O. N.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. External application of dsRNA targeting a Phakopsora pachyrhizi effector candidate attenuated fungal pathogenicity. In: INTERNATIONAL PLANT & ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. Abstracts... [S. l.: s. n.], 2017. não paginado. PLANT AND ANIMAL GENOME XXV CONFERENCE - INTLPAG, 2017.| Biblioteca(s): Embrapa Soja. |
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| 8. | | UTIYAMA, A. S.; ABE, V. Y.; DARBEN, L. M.; CARVALHO, K.; CARVALHO, M. C. C. G. de; MARCELINO-GUIMARÃES, F. C. Avaliação funcional de proteínas candidatas a efetores de Phakopsora pachyrhizi por meio da análise de espécies reativas a oxigênio (ROS) e deposição de calose. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 38-45.| Biblioteca(s): Embrapa Soja. |
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| 9. | | POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014.| Biblioteca(s): Embrapa Soja. |
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| 10. | | YOKOYAMA, A.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Diversidade genética de isolados monouredinias de Phakopsora pachyrhizi coletados em diferentes regiões do Brasil. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 11-15. (Embrapa Soja. Documentos, 339).| Biblioteca(s): Embrapa Soja. |
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| 11. | | CARVALHO, K.; ABE, V. Y.; DARBEN, L. M.; RINCÃO, M. P.; QI, M.; UTIYAMA, A. S.; CARVALHO, M. C. C. G.; LOPES, I. de O. N.; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Host induced gene silencing in P. pachyrhizi effectors interfere in virulence of soybean attenuating fungal pathogenicity In: IS-MPMI Congress, 18., 2019, Glasgow. [Abstracts...]. [S. l.: s. n.], 2019.| Biblioteca(s): Embrapa Soja. |
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| 12. | | KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; DARBEN, L. M.; SILVA, S. M. H.; CARVALHO, M. C. C. G. de; KUWAHARA, M. K.; OLIVEIRA, M. C. N. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Functional characterization of soybean GmHsp17.6B and GmHsp22.4 genes in response to Meloidogyne javanica infection. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 34.| Biblioteca(s): Embrapa Soja. |
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| 13. | | AOYAGI, L. N.; LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; DARBEN, L. M.; POLIZEL-PODANOSQUI, A.; KUWAHARA, M. K.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi. Plant Sciense, Limerick, v. 229, p. 32-42, Dec. 2014.| Biblioteca(s): Embrapa Soja. |
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| 14. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; KUWAHARA, M. K.; CARVALHO, M. C. C. G. de; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; GODOY, C. V.; MARCELINO-GUIMARÃES, F. C. Evaluation of virulence of Phakopsora pachyrhizi monourediniais isolates collected in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 15. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; GODOY, C. V.; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Molecular diversity of monouredinial Phakopsora pachyrhizi isolates collected in Brazil. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 56.| Biblioteca(s): Embrapa Soja. |
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| 16. | | RINCÃO, M. P.; CARVALHO, M. C. da C G. de; NASCIMENTO, L. C.; LOPES-CAITAR, V. S.; CARVALHO, K. de; DARBEN, L. M.; YOKOYAMA, A.; CARAZZOLLE, M. F.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta. Genetics and Molecular Biology, v. 41, n. 3, p. 671-691, 2018.| Biblioteca(s): Embrapa Soja. |
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| 17. | | CASTANHO, F. M.; KUWAHARA, M. K.; FERNANDES, G. P.; DARBEN, L. M.; YOKOYAMA, A.; SILVA, D. C. G. da; ABDELNOOR, R. V.; MEYER, M. C.; MARCELINO-GUIMARÃES, F. C. Characterization of Phakopsora pachyrhizi races from a broad geographical and temporal collection. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 105. p. 124.| Biblioteca(s): Embrapa Soja. |
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| 18. | | DARBEN, L. M.; YOKOYAMA, A.; CASTANHO, F. M.; LOPES-CAITAR, V. S.; CARVALHO, M. C. da C. G. de; GODOY, C. V.; CARVALHO, S. de; GONELA, A.; MARCELINO-GUIMARÃES, F. C. Characterization of genetic diversity and pathogenicity of Phakopsora pachyrhizi mono-uredinial isolates collected in Brazil. European Journal of Plant Pathology, v. 156, p. 355-372, 2020.| Biblioteca(s): Embrapa Soja. |
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| 19. | | CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. The transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 20. | | CARVALHO, M. C. D.; NASCIMENTO, L. C.; ROCHA, C. S.; CARAZZOLE, M. F.; DARBEN, L. M.; POLIZEL-PODANOSQUI, A. M.; PEREIRA, G. A.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Prediction of the secretome and effectors candidates of Phakopsora pachyrhizi during the course of infection in soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. [Anais...]. [Brasília]: Sociedade Brasileira de Fitopatologia, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 21 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
29/12/2014 |
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Data da última atualização: |
30/12/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
CNPSO - estagiário; UENP; CNPSO - estagiário; UENP; CNPSO - estagiário; CNPSO - estagiário; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO. |
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Título: |
Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Phakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust. MenosPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. T... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114606/1/Temporal-gene-expression-analysis-of-effector-candidates-of-Phakopsora-pachyrhizi-across-the-infection-cycle.pdf
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Marc: |
LEADER 02492nam a2200193 a 4500
001 2003884
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100 1 $aPOLIZEL-PODANOSQUI, A. M.
245 $aTemporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions$c2014
520 $aPhakopsora pachyrhizi is the causer of the Asian soybean rust, the most aggressive foliar disease that attacks the soybean plants in cultivated areas. To design effective breeding strategies for durable disease resistance it is important to understand the molecular basis of this plant-pathogen interaction. In particular, the characterization of the secreted effector proteins can help the breeding programs. Our recent analysis of the P. pachyrhizi secretome has revealed the presence of potential effectors genes. In this work, the temporal expression of 58 effector candidate genes were characterized from spores, germinated spores and infected soybean leaves at 0, 6, 12, 24, 36, 48, 72, 96, 192 and 240 hours after inoculation (hai) using RT-qPCR. Expression analysis showed differential temporal gene expression of these candidate effectors. Clustering analysis revealed three main clusters with coordinated expression of candidates across the infection cycle. The first has candidates expressed in specific steps of infection and almost sequences have the common motifs Y/F/WxC frequently found in haustoria-producing rust fungi effectors. The second cluster has candidates expressed during initial steps of infection that matches germ tube and appressorium formation (0 – 24 hai) and almost sequences have ?3 cysteine residues, other common features of the effectors. Third cluster has candidates expressed only in contact with the host, suggesting that these candidates are host-induced. The sequences in this cluster have the CFEM domain associated with pathogenicity. Functional analyses are in progress with more promising candidates effectors to validate the potential effectors from soybean rust.
653 $aFerrugem asiática da soja
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aROCHA, C. S.
700 1 $aDARBEN, L. M.
700 1 $aLOPES-CAITAR, V. S.
700 1 $aAOYAOGI, L. N.
700 1 $aMARCELINO-GUIMARÃES, F. C.
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