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Registros recuperados : 18 | |
8. | | DANTZGER, D. D.; DANTZGER, M.; JONSSON, C. M.; AOYAMA, H. In vitro effects of agriculture pollutants on microcrustacean and fish acid phosphatases. Water, Air, and Soil Pollution, v. 228, n. 10, p. 3-10, 2017. Article 391. Biblioteca(s): Embrapa Meio Ambiente. |
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10. | | DANTZGER, D. D.; CASTRO, V. L. S. S. de; JONSSON, C. M. Catalase biosensor and health environmental evaluation of agricultural pesticides. In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais...] São Pedro: Aptor Software, 2010. 1 CD-ROM. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas. Biblioteca(s): Embrapa Meio Ambiente. |
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12. | | DANTZGER, M.; DANTZGER, D. D.; JONSSON, C. M.; CASTRO, V. L. S. S. de; AOYAMA, H. The potential use of crustacean phosphatases as a biosensor to monitor metals in environmental samples. INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais...] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas. Biblioteca(s): Embrapa Meio Ambiente. |
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13. | | DANTZGER, M. D.; DANTZGER, D. D.; PRESTES, E. B.; JONSSON, C. M.; AOYAMA, H. Estudos de toxicidade de agroquímicos na atividade da fosfatase ácida de Daphnia similis. In: WORKSHOP DE ECOTOXICOLOGIA,, 3., 2008, Rio Claro. Resumos expandidos... Holos Environment, Rio Claro, v.8, n.2, supl., 2008. 4p. Biblioteca(s): Embrapa Meio Ambiente. |
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16. | | DANTZGER, D. D.; SA, L. A. N. de; PESSOA, M. C. P. Y.; ALMEIDA, G. R. de. Capacidade de infestação da cochonilha-do-carmim, Dactylopius ceylonicus, nas plantas hospedeiras Opuntia ficus-indica e Opuntia monacantha em condições de laboratório. In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 3., 2009, Campinas. Anais... Campinas: ITAL: IAC; Jaguariúna: Embrapa Meio Ambiente, 2009. 1 CD-ROM. Biblioteca(s): Embrapa Meio Ambiente. |
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17. | | VALLIM, J. H.; JONSSON, C. M.; SILVA, M. S. G. M. e; DANTZGER, D. D.; MARIGO, A. L. S.; CLEMENTE, Z.; AOYAMA, H. Ecotoxicologia do diflubenzuron: predição da concentração de baixo risco para organismos aquáticos. In: SIMPÓSIO DE ECOTOXICOLOGIA, 3., 2016, Rio Claro. Anais... Rio Claro, 2016. p. 36 Biblioteca(s): Embrapa Meio Ambiente. |
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18. | | JONSSON, C. M.; SILVA, M. S. G. M. e; MACEDO, V. S. de; DANTZGER, D. D.; VALLIM, J. H.; MARIGO, A. L. S.; AOYAMA, H. Prediction of a low-risk concentration of diflubenzuron to aquatic organisms and evaluation of clay and gravel in reducing the toxicity. Pan-American Journal of Aquatic Sciences, [S.l.], v. 10, n. 4, p. 259-272, 2015. Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 18 | |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
23/06/2017 |
Data da última atualização: |
01/08/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
DANTZGER, D. D.; JONSSON, C. M.; AOYAMA, H. |
Afiliação: |
D. D. DANTZGER; CLAUDIO MARTIN JONSSON, CNPMA; H. AOYAMA. |
Título: |
Analysis of enzymes as bioindicators on the combinatory effects of the mixtures of diflubenzuron and p-chloroaniline in tilapia in the presence of soil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 45., 2016, Natal. Anais... Natal: Sociedade brasileira de bioquímica e biologia molecular, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Introduction. Oreochromis niloticus (Nile tilapia) is the most produced and consumed fish in Brazil. Many fish farming use Diflubenzuron (DFB) as insecticide to control parasites responsible for diseases in these fish. This insecticide degradation generates p-chloroaniline (PCA), a potentially carcinogenic and mutagenic compound. Objectives The objective of this study was to evaluate the enzymes catalase (CAT) and transaminases (TGO and TGP) as water toxicity bioindicators using as experimental model the tilapia fish, exposed for 96 h to different combinatory concentrations of DFB and PCA mixtures, in presence of soil. Material and Methods. Samples of tilapia liver were homogenized and the supernatant was used for the biochemical analyses of enzyme activities and protein concentration. All the enzyme activities were determined by spectrophotometry. Results and Discussion. For catalase, in the presence of soil, 80% decrease was observed for the mixture of 25% DFB (75% PCA) and no significant effects were observed for the mixtures of 50 and 75% DFB, in relation to the control. At the same conditions no significant effects were observed on the transaminase TGO activities for all the mixtures of DFB tested. The TGP activities increased 350%, 80% and 60% for the mixtures of 25, 50 and 75% DFB, respectively, in relation to the control. Conclusions. Catalase and the transaminases TGO and TGP can be used as water toxicity bioindicators for the combinatory mixtures of DFB and PCA. The presence of the soil can dramatically change the activities of the enzymes studied. Finally, besides to serve as an alert, the data obtained in this work can be used to establish limit values for the mixture of these compounds in tropical freshwater bodies. MenosIntroduction. Oreochromis niloticus (Nile tilapia) is the most produced and consumed fish in Brazil. Many fish farming use Diflubenzuron (DFB) as insecticide to control parasites responsible for diseases in these fish. This insecticide degradation generates p-chloroaniline (PCA), a potentially carcinogenic and mutagenic compound. Objectives The objective of this study was to evaluate the enzymes catalase (CAT) and transaminases (TGO and TGP) as water toxicity bioindicators using as experimental model the tilapia fish, exposed for 96 h to different combinatory concentrations of DFB and PCA mixtures, in presence of soil. Material and Methods. Samples of tilapia liver were homogenized and the supernatant was used for the biochemical analyses of enzyme activities and protein concentration. All the enzyme activities were determined by spectrophotometry. Results and Discussion. For catalase, in the presence of soil, 80% decrease was observed for the mixture of 25% DFB (75% PCA) and no significant effects were observed for the mixtures of 50 and 75% DFB, in relation to the control. At the same conditions no significant effects were observed on the transaminase TGO activities for all the mixtures of DFB tested. The TGP activities increased 350%, 80% and 60% for the mixtures of 25, 50 and 75% DFB, respectively, in relation to the control. Conclusions. Catalase and the transaminases TGO and TGP can be used as water toxicity bioindicators for the combinatory mixtures of DFB and PCA. Th... Mostrar Tudo |
Palavras-Chave: |
Bioindicators; P-chloroaniline. |
Thesagro: |
Enzima. |
Thesaurus NAL: |
Diflubenzuron. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162225/1/dantzer-analysis.pdf
|
Marc: |
LEADER 02487nam a2200181 a 4500 001 2071398 005 2017-08-01 008 2016 bl uuuu u00u1 u #d 100 1 $aDANTZGER, D. D. 245 $aAnalysis of enzymes as bioindicators on the combinatory effects of the mixtures of diflubenzuron and p-chloroaniline in tilapia in the presence of soil.$h[electronic resource] 260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 45., 2016, Natal. Anais... Natal: Sociedade brasileira de bioquímica e biologia molecular$c2016 520 $aIntroduction. Oreochromis niloticus (Nile tilapia) is the most produced and consumed fish in Brazil. Many fish farming use Diflubenzuron (DFB) as insecticide to control parasites responsible for diseases in these fish. This insecticide degradation generates p-chloroaniline (PCA), a potentially carcinogenic and mutagenic compound. Objectives The objective of this study was to evaluate the enzymes catalase (CAT) and transaminases (TGO and TGP) as water toxicity bioindicators using as experimental model the tilapia fish, exposed for 96 h to different combinatory concentrations of DFB and PCA mixtures, in presence of soil. Material and Methods. Samples of tilapia liver were homogenized and the supernatant was used for the biochemical analyses of enzyme activities and protein concentration. All the enzyme activities were determined by spectrophotometry. Results and Discussion. For catalase, in the presence of soil, 80% decrease was observed for the mixture of 25% DFB (75% PCA) and no significant effects were observed for the mixtures of 50 and 75% DFB, in relation to the control. At the same conditions no significant effects were observed on the transaminase TGO activities for all the mixtures of DFB tested. The TGP activities increased 350%, 80% and 60% for the mixtures of 25, 50 and 75% DFB, respectively, in relation to the control. Conclusions. Catalase and the transaminases TGO and TGP can be used as water toxicity bioindicators for the combinatory mixtures of DFB and PCA. The presence of the soil can dramatically change the activities of the enzymes studied. Finally, besides to serve as an alert, the data obtained in this work can be used to establish limit values for the mixture of these compounds in tropical freshwater bodies. 650 $aDiflubenzuron 650 $aEnzima 653 $aBioindicators 653 $aP-chloroaniline 700 1 $aJONSSON, C. M. 700 1 $aAOYAMA, H.
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