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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/05/2009 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. |
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Afiliação: |
NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA/USP; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA/USP; TATIANA ALMEIDA DRUMOND TETZNER, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009. |
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DOI: |
10.1089/clo.2008.0044 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. MenosThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst ... Mostrar Tudo |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02377naa a2200205 a 4500
001 1048984
005 2023-03-15
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1089/clo.2008.0044$2DOI
100 1 $aSARAIVA, N. Z.
245 $aDemecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes.$h[electronic resource]
260 $c2009
520 $aThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine.
650 $aoocytes
700 1 $aPERECIN, F.
700 1 $aMÉO, S. C.
700 1 $aFERREIRA, C. R.
700 1 $aTETZNER, T. A. D.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells$gv. 11, n. 1, p. 141-152, mar. 2009.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
01/09/2015 |
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Data da última atualização: |
07/03/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BATISTA, E. dos S.; GARCIA, J. de S.; DAIRIKI, J. K.; INOUE, L. A. K. A. |
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Afiliação: |
Erix dos Santos Batista, Bolsista; Jailson de Sousa Garcia, Bolsista; JONY KOJI DAIRIKI, CPAA; LUIS ANTONIO KIOSHI AOKI INOUE, CPAA. |
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Título: |
Resposta metabólica do matrinxã (Brycon amazonicus) submetido ao óleo de alfavaca-cravo como anestésico. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 11., 2014, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 83-84. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo do trabalho foi avaliar as respostas metabólicas do matrinxã exposto a diferentes concentrações de óleo essencial. |
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Palavras-Chave: |
Estresse; Óleo de alfavaca-cravo. |
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Thesaurus NAL: |
Brycon amazonicus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/128899/1/XI-Jornada-IC-Resposta-metabolica.pdf
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Marc: |
LEADER 00811nam a2200181 a 4500
001 2023063
005 2016-03-07
008 2015 bl uuuu u00u1 u #d
100 1 $aBATISTA, E. dos S.
245 $aResposta metabólica do matrinxã (Brycon amazonicus) submetido ao óleo de alfavaca-cravo como anestésico.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 11., 2014, Manaus. Anais... Brasília, DF: Embrapa, 2015. p. 83-84.$c2015
520 $aO objetivo do trabalho foi avaliar as respostas metabólicas do matrinxã exposto a diferentes concentrações de óleo essencial.
650 $aBrycon amazonicus
653 $aEstresse
653 $aÓleo de alfavaca-cravo
700 1 $aGARCIA, J. de S.
700 1 $aDAIRIKI, J. K.
700 1 $aINOUE, L. A. K. A.
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