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| Registros recuperados : 75 | |
| 29. |  | JAMES, E. K.; OLIVARES, F. L.; BALDANI, J. I.; DOBEREINER, Johanna. Herbaspirillum, an endophytic diazotroph colonizing vascular tissue in leaves of Sorghum bicolor L. Moench. Journal of Experimental Botany, Oxford, v. 48, n. 308, p. 785-797, 1997.| Biblioteca(s): Embrapa Agrobiologia. |
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| 35. | | FREITAS, J. L. M. de; PEREIRA, P. A. A.; DOBEREINER, Johanna. Effects of organic matter and Azospirillum spp. strains in the metabolism of nitrogen in Sorghum vulgare. In: VOSE, P. B.; RUSCHEL, A. P., (Ed.). Associative N2-Fixation. Boca Raton: CRC, 1981. p. 155-163. Proceedings of the International Workshop of Associative N2-Fixation, July 2-6, 1979, held at Centro de Energia Nuclear na Agricultura, University of São Paulo, Piracicaba, Brasil.| Biblioteca(s): Embrapa Agrobiologia. |
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| 40. | | CARVALHO, A. R. V.; BALDANI, V. L. D.; REIS, V. M.; DÖBEREINER, Johanna. Bactérias diazotróficas em diferentes ecótipos de dendezeiro. In: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 23.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 7., SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 5., REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 2., out. 1998, Caxambu. Resumos... Lavras: UFLA / SBCS / SBM, 1998. p.199. 2. semestre. FERTBIO 98.| Biblioteca(s): Embrapa Agrobiologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/04/1999 |
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Data da última atualização: |
20/04/1999 |
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Autoria: |
OLIVARES, F. L.; JAMES, E. K.; BALDANI, J. I.; DOBEREINER, Johanna. |
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Título: |
Infection of mottled stripe disease-susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirillum. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
New Phytologist, Oxford, v. 135, p. 723-737, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Leaves of a mottled stripe disease-susceptible cultivar (B-4362) and of a mottled stripe disease-resistant cultivar (SP 70-1143) of sugar cane (interspecific hybrids of Saccharum) were inoculated with the diazotrophic endophytes, Herbaspirillum rubrisubalbicans or Herbaspirillum seropedicae, via injection into the apex of the stem. At 7 and 20 d.a.i., H. seropedicae could be isolated only from a small necrotic area around the point of inoculation, where there was considerable degradation of host cells, and was not detected in any other part of the leaves. This suggested a hypersensitive response by the host to this bacterium, and no disease symptoms formed on either cultivar. By contrast, H. rubrisubalbicans could be re-isolated from throughout the infectedleaves of both cultivars at both harvests and produced widespread disease symptoms on the leaves of cv. B-4362. Symptoms consisted of necrotic regions near the point of inoculation, and red patches along the vertical axis of the leaves, where the bacteria had spread in the primary and secondary veins. The xylem-conducing elements in disease regions of leaves were filled with bacteria and, at the edges of disease symptoms, the vessels were filled with a gum which stained blue-green with toluidine blue. This material probably contained phenolic compounds, and was produced as a host defence response. Leaves of cv. SP70-1143 only developed small red stripes near the point of inoculation. These symptoms did not spread along the leaves, and the infected xylem vessels were never seen to be completely full of bacteria, Instead, the vessels contained encapsulated bacterial colonies attached to secondary wall deposition; these colonies were surrounded by blue-green material that might have been host-defence gums. In cv. B-4362, bacteria were abundant in the intercellular spaces of mesophyll adjacent to infected xylem, and also filled sub-stomatal cavities. Immunogold labelling using polyclonal antisera raised against H. rubrisubalbicans gave a weak signal with the bacteria in cv. SP 70-1143, showing that few binding sites were available to the antibodies. By contrast, bacteria in cv. B-4362 reacted strongly with the antibody, suggesting that they had a denser coating of immunoreactive mucus. In the later stages of infection of cv. B-4362, lysed bacteria were seen within degrated plant cells surrounded by a matrix of plant gums and bacterial mucus. This matrix reacted strongly to the H. rubrisubalbicans antibodies. Immunogold labelling using antibodies against nitrogenase component II showed that nitrogenase was expressed by bacteria in the early stages of infection of cv. B-4362, but not in later stages, or by bacteria infecting cv. SP 70-1143. MenosLeaves of a mottled stripe disease-susceptible cultivar (B-4362) and of a mottled stripe disease-resistant cultivar (SP 70-1143) of sugar cane (interspecific hybrids of Saccharum) were inoculated with the diazotrophic endophytes, Herbaspirillum rubrisubalbicans or Herbaspirillum seropedicae, via injection into the apex of the stem. At 7 and 20 d.a.i., H. seropedicae could be isolated only from a small necrotic area around the point of inoculation, where there was considerable degradation of host cells, and was not detected in any other part of the leaves. This suggested a hypersensitive response by the host to this bacterium, and no disease symptoms formed on either cultivar. By contrast, H. rubrisubalbicans could be re-isolated from throughout the infectedleaves of both cultivars at both harvests and produced widespread disease symptoms on the leaves of cv. B-4362. Symptoms consisted of necrotic regions near the point of inoculation, and red patches along the vertical axis of the leaves, where the bacteria had spread in the primary and secondary veins. The xylem-conducing elements in disease regions of leaves were filled with bacteria and, at the edges of disease symptoms, the vessels were filled with a gum which stained blue-green with toluidine blue. This material probably contained phenolic compounds, and was produced as a host defence response. Leaves of cv. SP70-1143 only developed small red stripes near the point of inoculation. These symptoms did not spread along the... Mostrar Tudo |
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Palavras-Chave: |
Bacterias; BNF; FBN; Fixacao biologica de nitrogenio; Nitrogen fixing bacteria; Sugar cane. |
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Thesagro: |
Bactéria; Cana de Açúcar; Folha. |
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Thesaurus NAL: |
Herbaspirillum rubrisubalbicans; Herbaspirillum seropedicae; leaves. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03613naa a2200301 a 4500
001 1621027
005 1999-04-20
008 1997 bl uuuu u00u1 u #d
100 1 $aOLIVARES, F. L.
245 $aInfection of mottled stripe disease-susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirillum.
260 $c1997
520 $aLeaves of a mottled stripe disease-susceptible cultivar (B-4362) and of a mottled stripe disease-resistant cultivar (SP 70-1143) of sugar cane (interspecific hybrids of Saccharum) were inoculated with the diazotrophic endophytes, Herbaspirillum rubrisubalbicans or Herbaspirillum seropedicae, via injection into the apex of the stem. At 7 and 20 d.a.i., H. seropedicae could be isolated only from a small necrotic area around the point of inoculation, where there was considerable degradation of host cells, and was not detected in any other part of the leaves. This suggested a hypersensitive response by the host to this bacterium, and no disease symptoms formed on either cultivar. By contrast, H. rubrisubalbicans could be re-isolated from throughout the infectedleaves of both cultivars at both harvests and produced widespread disease symptoms on the leaves of cv. B-4362. Symptoms consisted of necrotic regions near the point of inoculation, and red patches along the vertical axis of the leaves, where the bacteria had spread in the primary and secondary veins. The xylem-conducing elements in disease regions of leaves were filled with bacteria and, at the edges of disease symptoms, the vessels were filled with a gum which stained blue-green with toluidine blue. This material probably contained phenolic compounds, and was produced as a host defence response. Leaves of cv. SP70-1143 only developed small red stripes near the point of inoculation. These symptoms did not spread along the leaves, and the infected xylem vessels were never seen to be completely full of bacteria, Instead, the vessels contained encapsulated bacterial colonies attached to secondary wall deposition; these colonies were surrounded by blue-green material that might have been host-defence gums. In cv. B-4362, bacteria were abundant in the intercellular spaces of mesophyll adjacent to infected xylem, and also filled sub-stomatal cavities. Immunogold labelling using polyclonal antisera raised against H. rubrisubalbicans gave a weak signal with the bacteria in cv. SP 70-1143, showing that few binding sites were available to the antibodies. By contrast, bacteria in cv. B-4362 reacted strongly with the antibody, suggesting that they had a denser coating of immunoreactive mucus. In the later stages of infection of cv. B-4362, lysed bacteria were seen within degrated plant cells surrounded by a matrix of plant gums and bacterial mucus. This matrix reacted strongly to the H. rubrisubalbicans antibodies. Immunogold labelling using antibodies against nitrogenase component II showed that nitrogenase was expressed by bacteria in the early stages of infection of cv. B-4362, but not in later stages, or by bacteria infecting cv. SP 70-1143.
650 $aHerbaspirillum rubrisubalbicans
650 $aHerbaspirillum seropedicae
650 $aleaves
650 $aBactéria
650 $aCana de Açúcar
650 $aFolha
653 $aBacterias
653 $aBNF
653 $aFBN
653 $aFixacao biologica de nitrogenio
653 $aNitrogen fixing bacteria
653 $aSugar cane
700 1 $aJAMES, E. K.
700 1 $aBALDANI, J. I.
700 1 $aDOBEREINER, Johanna.
773 $tNew Phytologist, Oxford$gv. 135, p. 723-737, 1997.
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