Data/hora: 25/06/2024 00:46:35

Biblioteca(s): | Embrapa Agroenergia.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 4
Data corrente: | 10/12/2021
Data da última atualização: | 10/12/2021
Autoria: | RAMOS, T. G. S.; JUSTEN, F.; CARNEIRO, C. V. G. C.; HONORATO, V. M.; FRANCO, P. F.; VIEIRA, F. S.; TRICHEZ, D.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de.
Afiliação: | TALITA G.S. RAMOS, Universidade de Brasília; FERNANDA JUSTEN, Universidade de Brasília; CLARA V. G. C. CARNEIRO, Universidade de Brasília; VICTOR M. HONORATO, Universidade de Brasília; PAULA FERNANDES FRANCO, CNPAE; FLÁVIA S. VIEIRA; DÉBORA TRICHEZ; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE.
Título: | Xylonic acid production by recombinant Komagataella phaffii strains engineered with newly identified xylose dehydrogenases.
Ano de publicação: | 2021
Fonte/Imprenta: | Bioresource Technology Reports, v. 16, 100825, Dec. 2021.
Páginas: | 6 p.
Descrição Física: | PDF: il.
ISSN: | 2589-014X
DOI: | https://doi.org/10.1016/j.biteb.2021.100825
Idioma: | Inglês
Conteúdo: | Abstract: Microbial production of xylonic acid can be achieved via xylose oxidation catalyzed by the xylose dehydrogenase enzyme. In the present study, 11 putative xylose dehydrogenases (XDH) from bacterial and fungi were identified through in silico analysis using the sequences of the two XDH previously described. Among them, 6 genes were successfully cloned and expressed in Komagataella phaffii (Pichia pastoris) as demonstrated by the ability of the strains to produce xylonic acid. The best strain was capable of producing up to 37.1 ± 1.9 and 11.7 ± 1.6 g/L of xylonic acid with yields of 0.96 ± 0.02 and 0.40 ± 0.06 g/g in mineral medium and sugarcane bagasse hydrolysate, respectively. The results presented here demonstrated the functionality of 6 newly identified xylose dehydrogenases, increasing the number of XDH described. In addition, the efficiency of K. phaffii as a xylonic acid producer is showed for the first time.
Palavras-Chave: | Biomass hydrolysate; Komagataella phaffii; Xylonic acid; Xylose dehydrogenase.
Thesaurus Nal: | Biomass; Hydrolysates.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3965-1) - UPC - AP - DD -  -  -  - DIS