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10. | | ANDREOTTI, R.; SAIMO-KAHWA, M.; CUNHA, R. C.; BARROS, J. C.; LEITE, F. P. L. Characterization and Production of recombinant protein Ra92A from Rhipicephalus appendiculatus aiming tick vaccine antigen in Uganda. In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 13., 2014, Mexico. Conference abstracts... Mexico City, Mexico: Academic Committee ICOPA Congress, 2014. Biblioteca(s): Embrapa Gado de Corte. |
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13. | | CUNHA, R. C.; LEITE, F. P. L.; PINTO, L. da S.; SATO, T.; ANDREOTTI, R. Subclonagem do gene Bm86-CG em pPIC9 para expressão heteróloga em Pichia pastoris. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Foz do Iguaçu: SBP, 2009. p. 1298. Revista Patologia Tropical, v. 38, jul.-set 2009. Suplemento 2. Biblioteca(s): Embrapa Gado de Corte. |
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18. | | ANDREOTTI, R.; GARCIA, M. V.; CUNHA, R. C.; BARROS, J. C. Action of Tagetes minuta (Asteraceae) essential oil in the control of Rhipicephalus microplus (Acari: ixodidae). In: INTERNATIONAL SYMPOSIUM ON TICK CONTROL AND TICK-BORNE DISEASES, 2013, Campo Grande, MS. [Proceedings..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 202). Comissão organizadora: Renato Andreotti, Fernando Paiva, Wilson Werner Koller, Jacqueline Cavalcante Barros, Luiz Antonio Dias Leal, Jaqueline Matias. Biblioteca(s): Embrapa Gado de Corte. |
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19. | | CUNHA, R. C.; ANDREOTTI, R.; SILVA, E. A. e; ELISÂNGELA PEREIRA; SATO, T.; THOMAZ-SOCCOL, V. Laboratory diagnosis and clinical signs of canine visceral leishmaniasis in dogs examined at the center for zoonosis control in Campo Grande - MS, Brazil. Archives of Veterinary Science, v. 17, n. 4, p. 17-26, 2012. Título em português: Diagnóstico laboratorial e sinais clínicos para Leishmaniose visceral canina em cães examinados no Centro de Controle de Zoonoses de Campo Grande - MS. Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Gado de Corte. |
Data corrente: |
28/01/2020 |
Data da última atualização: |
06/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GIACHETTO, P. F.; CUNHA, R. C.; NHANI JUNIOR, A.; GARCIA, M. V.; FERRO, J. A.; ANDREOTTI, R. |
Afiliação: |
POLIANA FERNANDA GIACHETTO, CNPTIA; RODRIGO CASQUERO CUNHA, UFPEL; ANTONIO NHANI JUNIOR, CNPTIA; MARCOS VALERIO GARCIA, CNPGC; JESUS APARECIDO FERRO, UNESP; RENATO ANDREOTTI E SILVA, CNPGC. |
Título: |
Gene expression in the salivary gland of Rhipicephalus (Boophilus) microplus fed on tick-susceptible and tick-resistant hosts. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-15, Jan. 2020. |
DOI: |
10.3389/fcimb.2019.0047 |
Idioma: |
Inglês |
Notas: |
Article 477. |
Conteúdo: |
The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding. MenosThe success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed ... Mostrar Tudo |
Palavras-Chave: |
Bioinformática; Cattle tick; Expressão gênica; Host-parasite interaction; RNA-Seq. |
Thesagro: |
Carrapato; Gado de Corte. |
Thesaurus NAL: |
Bioinformatics; Cattle; Rhipicephalus microplus; Sialome; Transcriptome. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215164/1/AP-Gene-expression-in-the-salivary-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209727/1/Gene-expression-in-the-salivary.pdf
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Marc: |
LEADER 03526naa a2200349 a 4500 001 2124241 005 2020-11-06 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3389/fcimb.2019.0047$2DOI 100 1 $aGIACHETTO, P. F. 245 $aGene expression in the salivary gland of Rhipicephalus (Boophilus) microplus fed on tick-susceptible and tick-resistant hosts.$h[electronic resource] 260 $c2020 500 $aArticle 477. 520 $aThe success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na+/dicarboxylate, Na+/tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding. 650 $aBioinformatics 650 $aCattle 650 $aRhipicephalus microplus 650 $aSialome 650 $aTranscriptome 650 $aCarrapato 650 $aGado de Corte 653 $aBioinformática 653 $aCattle tick 653 $aExpressão gênica 653 $aHost-parasite interaction 653 $aRNA-Seq 700 1 $aCUNHA, R. C. 700 1 $aNHANI JUNIOR, A. 700 1 $aGARCIA, M. V. 700 1 $aFERRO, J. A. 700 1 $aANDREOTTI, R. 773 $tFrontiers in Cellular and Infection Microbiology$gv. 9, p. 1-15, Jan. 2020.
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