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| Registros recuperados : 11 | |
| 3. |  | MARTINS, K. B.; FACCIOLI, P. Y.; BONESSO, M. F.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. Journal of Dairy Science, v. 100, n. 3, p. 2184-2195, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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| 4. | | MELLO, P. L.; FACCIOLI-MARTINS, P. Y.; LAMEIDA M. L. de; BRITO, M. A. V. P.; CUNHA, M. de L. R. de S. da. Determination of resistance and virulence in Staphylococcus spp. Isolated from bovine mastitis in different Brazilian states Hechos Microbiológicos, v. 5, n. 2, p. 114-115, 2014. Suplemento. Resumen TLP-543. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia (ALAM) e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 5. | | MARLIN, K. B.; PEREIRA, V. C.; OLIVEIRA, A. de; FERNANDES, S. J.; FACCIOLI, P. Y.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Evaluation of the presence of the bap gene in staphylococcus isolated from ewe subclinical mastitis milk. In: INTERNATIONAL SYMPOSIUM ON STAPHYLOCOCCI AND STAPHYLOCOCCAL INFECTIONS, 2012, Lyon. Abstract... Lyoon: Universite de Lyon, 2012.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | MELLO, P. L.; RIBOLI, D. F. M.; PINHEIRO, L.; MARTINS, L. de A.; BRITO, M. A. V. P. e; CUNHA, M. de L. R. de S. da. Detection of enterotoxigenic potential and determination of clonal profile in Staphylococcus aureus and Coagulase-Negative Staphylococci isolated from bovine subclinical mastitis in different Brazilian states. Toxins, v. 8, n. 4, article 104, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | PIRES, F. V.; CUNHA, M. de L. R. de S. da; ABRAÃO, L. M.; FACCIOLI-MARTINS, P. Y.; CAMARGO, C. H.; FORTALEZA, C. M. C. B. Nasal carriage of Staphylococcus aureus in Botucatu, Brazil: a population-based survey. PLOS ONE, v. 9, n. 3, e92537, Marc. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | FERREIRA, E. M.; ROMERO, L. C.; CUNHA, M. DE L. R. DE S. DA; MALAGO JUNIOR, W.; CAMARGO, C. H.; BARIONI JUNIOR, W.; ZAFALON, L. F. Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis. BMC Veterinary Research, v. 18, n. 273, 2022. 13 p.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | ZAFALON, L. F.; CUNHA, M. de L. R. de S. da; TEIXEIRA, N. B.; GARCES, H. G.; YAMAUCHI, D. H.; SILVA, L. P.; FERREIRA, E. M. Toxin-encoding genes of Staphylococcus Spp. Isolated from milk of cows treated with homeopathy. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió, Anais... Maceió: SBM, 2019.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | MARTINS, K. B.; FACCIOLI-MARTINS, P. Y.; RIBOLI, D. F. M.; PEREIRA, V. C.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk. Brazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, apr./jun. 2015.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | FERREIRA, E. M.; ROMERO, L. C.; SILVA, L. P.; PEREIRA, L. E. C.; MALAGO JUNIOR, W.; CHAPAVAL, L.; CUNHA, M. de L. R. de S. da; ZAFALON, L. F. Genotypic and phenotypic characterization of antimicrobial resistance in Staphylococcus Spp. isolated from cows after alternative treatment of bovine mastitis. In: INTERNATIONAL SYMPOSIUM ON VETERINARY SCIENCE, 2019, Viçosa. Proceedings... Viçosa: UFV, 2019. p.164| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 11 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
26/11/2015 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MARTINS, K. B.; FACCIOLI-MARTINS, P. Y.; RIBOLI, D. F. M.; PEREIRA, V. C.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. |
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Afiliação: |
KATHERYNE BENINI MARTINS, Universidade Estadual Paulista; PATRICIA YOSHIDA FACCIOLI-MARTINS, Universidade Estadual Paulista; DANILO FLÁVIO MORAES RIBOLI, Universidade Estadual Paulista; VALÉRIA CATANELI PEREIRA, Universidade Estadual Paulista; SIMONE FERNANDES, Universidade Estadual Paulista; ALINE A. OLIVEIRA, Universidade Estadual Paulista; ARIANE DANTAS, Universidade Estadual Paulista; LUIZ FRANCISCO ZAFALON, CPPSE; MARIA DE LOURDES RIBEIRO DE SOUZA DA CUNHA, Universidade Estadual Paulista. |
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Título: |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, apr./jun. 2015. |
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DOI: |
10.1590/S1517-838246220131164 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene. MenosThe objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of ... Mostrar Tudo |
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Palavras-Chave: |
Virulence factors. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus NAL: |
antibiotic resistance; mastitis; sheep. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134092/1/ZAFALON2.pdf
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Marc: |
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001 2029706
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008 2015 bl uuuu u00u1 u #d
024 7 $a10.1590/S1517-838246220131164$2DOI
100 1 $aMARTINS, K. B.
245 $aClonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk.$h[electronic resource]
260 $c2015
520 $aThe objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.
650 $aantibiotic resistance
650 $amastitis
650 $asheep
650 $aStaphylococcus Aureus
653 $aVirulence factors
700 1 $aFACCIOLI-MARTINS, P. Y.
700 1 $aRIBOLI, D. F. M.
700 1 $aPEREIRA, V. C.
700 1 $aFERNANDES, S.
700 1 $aOLIVEIRA, A. A.
700 1 $aDANTAS, A.
700 1 $aZAFALON, L. F.
700 1 $aCUNHA, M. de L. R. de S. da
773 $tBrazilian Journal of Microbiology$gv. 46, n. 2, p. 535-543, apr./jun. 2015.
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