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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/10/2011 |
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Data da última atualização: |
12/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, A. C. Q.; MORGANTE, C. V.; SANTOS, C. M. R.; SILVA, F. R. da; ARAÚJO, A. C. G.; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
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Afiliação: |
UnB; CAROLINA VIANNA MORGANTE, CPATSA; UnB; UnB; UnB; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A transcriptional approach for identifying genes related to drought stress. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. |
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Páginas: |
p. 77. |
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Idioma: |
Inglês |
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Conteúdo: |
Stress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease responsive protein was down-regulated. The information produced in this study is a valuable resource for gene identi6cation, characterization of new wild alleles, and development of molecular markers. MenosStress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease res... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Estresse hídrico. |
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Thesagro: |
Amendoim; Marcador Molecular; Planta. |
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Thesaurus Nal: |
Peanuts. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131239/1/ID-45694.pdf
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Marc: |
LEADER 02684nam a2200289 a 4500
001 1903303
005 2023-06-12
008 2011 bl uuuu u00u1 u #d
100 1 $aMARTINS, A. C. Q.
245 $aA transcriptional approach for identifying genes related to drought stress.
260 $aIn: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology$c2011
300 $ap. 77.
520 $aStress-responsive mechanisms triggered in plants under drought stress affect plant growth and cause serious limitation to crop productivity. Due to its high genetic diversity and adaptation to a range of environments, wild relatives of peanut constitute a rich source of allele diversity for resistance to biotic and abiotic stresses. Based on previous data and as a 6rst step to identify drought-responsive genes inArachis, the wild specieA. magna, accession KG30097, was selected as it showed high adaptability to water stress conditions. The transcriptome of A. magna leaves subrnitted to gradual water stress was analyzed. Subtractive libraries were constructed with cDNA from leaf tissues of stressed and well-watered control plants. Subtractive hybridization was performed in both directions: cD A from stressed plants was used as driver and afterwards as tester, allowing for the enrichment of genes either induced or inhibited during water stress. In silicoanalysis revealed 759 reads, which were grouped into 249 clusters, with a novelty index of 32,8%. Several up and downregulated genes were identi6ed exclusively in stressed or control conditions. The expression proíile of some differentially regulated genes was validated by real time PCR, using cDNA Erom roots and leafs of stressed and control plants. Glycine descarboxilase, metallothionein-like protein, drought stress responsive protein, and two unknown proteins were shown to be up-regulated and the gene coding for a disease responsive protein was down-regulated. The information produced in this study is a valuable resource for gene identi6cation, characterization of new wild alleles, and development of molecular markers.
650 $aPeanuts
650 $aAmendoim
650 $aMarcador Molecular
650 $aPlanta
653 $aDiversidade genética
653 $aEstresse hídrico
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, C. M. R.
700 1 $aSILVA, F. R. da
700 1 $aARAÚJO, A. C. G.
700 1 $aBERTIOLI, D. J.
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aGUIMARAES, P. M.
700 1 $aBRASILEIRO, A. C. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
30/11/2011 |
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Data da última atualização: |
18/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CASTRO, M. de J. P. de; CRUZ, P. L.; FANELA, T. L. M.; BALDIN, E. L. L.; TAKEARA, R.; SILVA, P. H. S. da. |
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Afiliação: |
MARIA DE JESUS PASSOS DE CASTRO, FCA/UNESP; PATRICIA LEITE CRUZ, FCA/UNESP; THIAGO LUIS MARTINS FANELA, FCA/UNESP; EDSON LUIZ LOPES BALDIN, FCA/UNESP; RENATA TAKEARA, UFAM; PAULO HENRIQUE SOARES DA SILVA, CPAMN. |
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Título: |
Toxicidade do óleo essencial de Piper callosum sobre a sobrevivência de Acanthoscelides obtectus Say (Coleoptera: Bruchidae). |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE ÓLEOS ESSENCIAIS, 6., 2011, Campinas. Livro de resumos. Campinas: Unicamp, 2011. |
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Páginas: |
p. 81. |
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Idioma: |
Português |
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Palavras-Chave: |
Atividade inseticida; Feijão armazenado. |
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Thesagro: |
Óleo Essencial. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48480/1/Simposio20001.pdf
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Marc: |
LEADER 00714nam a2200205 a 4500
001 1907852
005 2023-12-18
008 2011 bl uuuu u00u1 u #d
100 1 $aCASTRO, M. de J. P. de
245 $aToxicidade do óleo essencial de Piper callosum sobre a sobrevivência de Acanthoscelides obtectus Say (Coleoptera$bBruchidae).
260 $aIn: SIMPÓSIO BRASILEIRO DE ÓLEOS ESSENCIAIS, 6., 2011, Campinas. Livro de resumos. Campinas: Unicamp$c2011
300 $ap. 81.
650 $aÓleo Essencial
653 $aAtividade inseticida
653 $aFeijão armazenado
700 1 $aCRUZ, P. L.
700 1 $aFANELA, T. L. M.
700 1 $aBALDIN, E. L. L.
700 1 $aTAKEARA, R.
700 1 $aSILVA, P. H. S. da
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Embrapa Meio-Norte (CPAMN) |
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