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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
12/07/1997 |
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Data da última atualização: |
18/06/2014 |
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Autoria: |
RACHWAL, M. F. G.; DEDECEK, R. A.; MONTOYA VILCAHUAMAN, L. J.; CURCIO, G. R. |
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Afiliação: |
EMBRAPA-CNPF. |
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Título: |
Influência das características químicas e fisico-hídricas de um solo degradado por mineração de xisto sobre a altura e sobrevivencia de leguminosas arbóreas. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 25., 1995, VIÇOSA, MG. O Solo nos Grandes Domínios Morfoclimáticos do Brasil e o Desenvolvimento Sustentado: resumos expandidos. Viçosa, MG: Sociedade Brasileira do Solo: Universidade Federal de Viçosa, Departamento de Solos, 1995. v. 4, p. 2372-2375. |
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Idioma: |
Português |
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Palavras-Chave: |
Característica fisico-hidrica; Leguminosa arbórea; Solo degradado. |
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Thesagro: |
Mineração; Xisto. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/103863/1/InfluenciaCaracteristicas0001.pdf
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Marc: |
LEADER 00946naa a2200205 a 4500
001 1300358
005 2014-06-18
008 1995 bl uuuu u00u1 u #d
100 1 $aRACHWAL, M. F. G.
245 $aInfluência das características químicas e fisico-hídricas de um solo degradado por mineração de xisto sobre a altura e sobrevivencia de leguminosas arbóreas.
260 $c1995
650 $aMineração
650 $aXisto
653 $aCaracterística fisico-hidrica
653 $aLeguminosa arbórea
653 $aSolo degradado
700 1 $aDEDECEK, R. A.
700 1 $aMONTOYA VILCAHUAMAN, L. J.
700 1 $aCURCIO, G. R.
773 $tIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 25., 1995, VIÇOSA, MG. O Solo nos Grandes Domínios Morfoclimáticos do Brasil e o Desenvolvimento Sustentado: resumos expandidos. Viçosa, MG: Sociedade Brasileira do Solo: Universidade Federal de Viçosa, Departamento de Solos, 1995.$gv. 4, p. 2372-2375.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; NAIARA ZOCCAL SARAIVA, UNESP; MARIA HELENA COELHO CRUZ, UNESP; BRUNA MAZETI, UNESP; LETICIA ZOCCOLARO OLIVEIRA, UNESP; FLAVIA LOMBARDI LOPES, UNESP; JOAQUIM MANSANO GARCIA, UNESP. |
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Título: |
HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Reproduction, v. 145, n. 1, p. 9-17, 2013. |
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DOI: |
https://doi.org/10.1530/rep-11-0343 |
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Idioma: |
Inglês |
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Conteúdo: |
During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos. MenosDuring initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained u... Mostrar Tudo |
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Palavras-Chave: |
Cultured Female Fertilization; Drug effects. |
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Thesaurus NAL: |
Blastocyst; Embryology; Metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02665naa a2200265 a 4500
001 1978655
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1530/rep-11-0343$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aHDAC inhibition decreases XIST expression on female IVP bovine blastocysts.$h[electronic resource]
260 $c2013
520 $aDuring initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos.
650 $aBlastocyst
650 $aEmbryology
650 $aMetabolism
653 $aCultured Female Fertilization
653 $aDrug effects
700 1 $aSARAIVA, N. Z.
700 1 $aCRUZ, M. H. C.
700 1 $aMAZETI, B.
700 1 $aOLIVEIRA, L. Z.
700 1 $aLOPES, F. L.
700 1 $aGARCIA, J. M.
773 $tReproduction$gv. 145, n. 1, p. 9-17, 2013.
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