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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
05/03/2013 |
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Data da última atualização: |
11/03/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRASILEIRO, A. C. M.; MORGANTE, C. V.; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; PAPPAS, G.; SILVA JUNIOR, O. B. da; MARTINS, A. C. Q.; BERTIOLI, D.; GUIMARAES, P. M. |
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Afiliação: |
ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CAROLINA VIANNA MORGANTE, CPATSA; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ABADIO HERMES VIEIRA, CPAF-RO; PATRICIA MESSEMBERG GUIMARAES, CENARGEN. |
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Título: |
Transcriptome analysis in response to gradual water deficit in Arachis wild relatives. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Peanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization of new alleles and development of molecular markers in Arachis. MenosPeanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization ... Mostrar Tudo |
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Palavras-Chave: |
Cultura resistente a seca; Drought-prone areas; Melhoramento genético; Peanut; Tolerância à seca. |
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Thesagro: |
Amendoim; Arachis Hypogaea; Melhoramento genético vegetal; Planta oleaginosa. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77977/1/MORGANTE0002.pdf
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Marc: |
LEADER 02609nam a2200313 a 4500
001 1952220
005 2013-03-11
008 2012 bl uuuu u00u1 u #d
100 1 $aBRASILEIRO, A. C. M.
245 $aTranscriptome analysis in response to gradual water deficit in Arachis wild relatives.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT$c2012
520 $aPeanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization of new alleles and development of molecular markers in Arachis.
650 $aAmendoim
650 $aArachis Hypogaea
650 $aMelhoramento genético vegetal
650 $aPlanta oleaginosa
653 $aCultura resistente a seca
653 $aDrought-prone areas
653 $aMelhoramento genético
653 $aPeanut
653 $aTolerância à seca
700 1 $aMORGANTE, C. V.
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aARAUJO, A. C. G. de
700 1 $aPAPPAS, G.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aMARTINS, A. C. Q.
700 1 $aBERTIOLI, D.
700 1 $aGUIMARAES, P. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
04/12/2001 |
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Data da última atualização: |
13/03/2020 |
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Autoria: |
CRUZ, A. R. R. |
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Título: |
Micropropagacao de oito genotipos de abacaxizeiro [Ananas comosus (L.) Merril]. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Brasilia: UnB, 2000 |
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Páginas: |
71p. |
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Idioma: |
Português |
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Notas: |
Tese Mestrado (Agronomia). Orientador: Afonso Celso C. Valois. |
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Palavras-Chave: |
Biorreator. |
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Thesagro: |
Abacaxi; Ananás Comosus; Micropropagação. |
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Thesaurus NAL: |
bioreactors; micropropagation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00563nam a2200193 a 4500
001 1180219
005 2020-03-13
008 2000 bl uuuu m 00u1 u #d
100 1 $aCRUZ, A. R. R.
245 $aMicropropagacao de oito genotipos de abacaxizeiro [Ananas comosus (L.) Merril].
260 $aBrasilia: UnB$c2000
300 $a71p.
500 $aTese Mestrado (Agronomia). Orientador: Afonso Celso C. Valois.
650 $abioreactors
650 $amicropropagation
650 $aAbacaxi
650 $aAnanás Comosus
650 $aMicropropagação
653 $aBiorreator
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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