Data/hora: 16/06/2024 22:43:50

Biblioteca(s): | Embrapa Gado de Corte.
Data corrente: | 05/11/2001
Data da última atualização: | 20/02/2009
Autoria: | MADRUGA, C. R.; MARQUES, A. P. C.; ARAUJO, F. R.; MIGUITA, M.; CARVALHO, C. M. E.; ARAUJO, F. S.; UMAKI, A. C. S.; CROCCI, A. J.; QUEIROZ, R. A.
Afiliação: | Embrapa Gado de Corte (Campo Grande, MS).
Título: | Evaluation of an ELISA for detection of antibodies to Babesia bigemin in cattle and it's application in an epidemiological survey in Brazil.
Ano de publicação: | 2001
Fonte/Imprenta: | Pesquisa Veterinaria Brasileira, Rio de Janeiro, v.21, n.2, p.72-76, abr. / jun. 2001.
Idioma: | Inglês
Notas: | CNPGC.
Conteúdo: | An indirect  enzyme-linked  immunosorbent assay (ELISA) using a crude antigen was evaluated  of its performance to detect Babesia antibodies. The sensitivity and specificity were 98,0% and 99,0%, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 10 Babesia bovis organisms. With regard to the comparison of ELISA and  indirect fluorescent antibody test. (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves sera. There was a good agreement between results shown by ELISA and IFAT with sera  from an enzootically stable area (K=0,61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (K=0,33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four  counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalence ranged from 87,7 to 98,9%. Therefore, this ELISA with high sensitivity, specificity  and performance similar to IFAT can be employed in serological  diagnosis of B. bigemina.
Palavras-Chave: | Brasil; IFAT; Immunofluorescense; Mato Grosso do Sul.
Thesagro: | Babesia Bigemina; Bovino; Elisa; Epidemiologia; Imunofluorescência; Imunologia.
Thesaurus Nal: | Brazil; cattle; epidemiology; immunology; Pantanal.
Categoria do assunto: | --
Registro original: | Embrapa Gado de Corte (CNPGC)
Exemplares: | CNPGC - (8878-1) - UPC - AP - -- - 636.08905 -  - 636 - DIS