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| Registros recuperados : 55 | |
| 6. |  | COUTO, R. I.; SENO, S.; SELEGUINI, A. Espaçamento e cultivares de alface conduzidas no verão em ambiente protegido na região de Ilha Solteira-SP. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 278, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 9. | | COELHO, Y. S.; COUTO, R. C. S.; NICKEL, O. Ocorrencia do declinio dos citros em um agroecossistema florestal no Sul da Bahia Curitiba, PR: IAPAR/SBF, 1996 p.152 In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 14. REUNIAO INTERAMERICANA DE HORTICULTURA TROPICAL, 42, 1996, Curitiba, PR| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | DAYRELL, M. de S.; FERREIRA, A. de M.; COUTO, R. de C. A.; IVAN, M. Niveis de cobre, cobalto, ferro e manganes no figado de bovinos e nas forrageiras de propriedades da regiao do Alto Sao Francisco-MG. Campinas: SBZ, 1990. p.52. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 27., 1990, Campinas. Anais...| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | OLIVEIRA, D. G. de; REIS, E. F. dos; MEDEIROS, J. C.; COUTO, R. F.; HOLTZ, V.; MADARI, B. E. Correlação espacial e linear de atributos físicos do solo em sistema de integração lavoura pecuária. Ciência Agrícola, Rio Largo, v. 15, n. 1, p. 69-77, 2017.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 18. | | MARTINS, R. P.; COMIN, J. J.; GATIBONI, L. C.; SOARES, C. R. F.; COUTO, R. da R.; BRUNETTO, G. Mineralização do nitrogênio de plantas de cobertura, solteiras e consorciadas, depositadas sobre um solo com histórico de cultivo de cebola. Revista ceres, Viçosa, v. 61, n. 4, p. 587-596, jul/ago, 2014.| Biblioteca(s): Embrapa Hortaliças. |
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| 20. | | CASAL, A. V.; MELO, G. W. B. de; AMBROSINI, V. G.; COUTO, R. da R.; BRUNETTO, G. Estado nutricional, produção e composição das uvas de ?Niágara Rosada? submetidas à aplicação de composto orgânico. Revista de Ciências Agrárias, Belém, v. 58, n. 3, p. 257-262, jul./set. 2015.| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 55 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
23/05/2018 |
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Data da última atualização: |
23/05/2018 |
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Autoria: |
FERREIRA, B. C.; ECCO, R.; COUTO, R. M.; COELHO, H. E.; ROSSI, D. A.; BELETTI, M. E.; SILVA, P. L. |
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Afiliação: |
Bruna C. Ferreira, Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia - UFU; Roselene Ecco, Setor de Patologia Veterinária, Escola de Veterinária, Laboratório de Histopatologia, Universidade Federal de Minas Gerais - UFMG; Rodrigo M. Couto, Setor de Patologia Veterinária, Escola de Veterinária, Laboratório de Histopatologia, Universidade Federal de Minas Gerais - UFMG; Humberto E. Coelho, Setor de Patologia Veterinária, Universidade de Uberaba - Uniube; Daise A. Rossi, Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia - UFU; Marcelo E. Beletti, Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia - UFU; Paulo L. Silva, Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia - UFU. |
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Título: |
Outbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 3, p. 417-424, março 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Surto de bouba aviária na forma cutânea em perus comerciais previamente vacinados. |
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Conteúdo: |
This study describes an outbreak of avian poxvirus disease in previously pox-vaccinated turkeys in Brazil. The turkeys had suggestive gross lesions of cutaneous avian poxvirus in the skin of the head and cervical area without changes in the flock mortality rates. In the slaughterhouse, 30 carcasses were removed from the slaughter line to collect tissue from cutaneous lesions for histological analyses and characterization of the virus. The virus was identified by conventional polymerase chain reaction (PCR) and subsequent gene sequencing. Acanthosis, hyperkeratosis, and hydropic degeneration were seen on skin histopathology. Eosinophilic intracytoplasmic inclusion bodies (Bollinger) on keratinocytes were observed in 46.6% of the samples. Avian poxvirus DNA was detected on PCR in 83.3% of the total samples. PCR associated with histopathology had 93.3% of positivity for avian poxvirus. In the phylogenetic study, samples show 100% matching suggesting that the outbreak occurred by a single viral strain and was different from those strains affecting other wild birds such as canaries and sparrows. A single mutation (Adenine for Guanine) was detected in our study?s strain and in the strains of turkey, chickens, and vaccine strains published in GenBank. Also, when the sequence strain of the present study and sequences from GenBank of canarypox and sparrowpox strains were aligned, a Thymine was found replacing the Adenine or Guanine. The in ovo vaccination method as single-use in turkeys of this study apparently did not provide adequate protection against avianpox disease, but additional vaccination administered by wing-web when turkeys were 45-60 days old in the new flocks controlled the disease. In the subsequent year, new cases of this disease were not found. It was not possible to confirm the source of the virus strain, but infection with a field strain derived from chickens is one possibility, considering the poultry farm population in the area and biosecurity aspects. For wide characterization of avipoxvirus and differentiation among strains, the complete sequence of the viral genome is required. MenosThis study describes an outbreak of avian poxvirus disease in previously pox-vaccinated turkeys in Brazil. The turkeys had suggestive gross lesions of cutaneous avian poxvirus in the skin of the head and cervical area without changes in the flock mortality rates. In the slaughterhouse, 30 carcasses were removed from the slaughter line to collect tissue from cutaneous lesions for histological analyses and characterization of the virus. The virus was identified by conventional polymerase chain reaction (PCR) and subsequent gene sequencing. Acanthosis, hyperkeratosis, and hydropic degeneration were seen on skin histopathology. Eosinophilic intracytoplasmic inclusion bodies (Bollinger) on keratinocytes were observed in 46.6% of the samples. Avian poxvirus DNA was detected on PCR in 83.3% of the total samples. PCR associated with histopathology had 93.3% of positivity for avian poxvirus. In the phylogenetic study, samples show 100% matching suggesting that the outbreak occurred by a single viral strain and was different from those strains affecting other wild birds such as canaries and sparrows. A single mutation (Adenine for Guanine) was detected in our study?s strain and in the strains of turkey, chickens, and vaccine strains published in GenBank. Also, when the sequence strain of the present study and sequences from GenBank of canarypox and sparrowpox strains were aligned, a Thymine was found replacing the Adenine or Guanine. The in ovo vaccination method as single-use in turk... Mostrar Tudo |
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Palavras-Chave: |
Fpv167 gene; Gene fpv167; Pathology; Patologia; Pox-vaccinated; Sequenciamento; Sequencing. |
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Thesagro: |
Bouba Aviaria; Peru; Vacina. |
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Thesaurus NAL: |
Avipoxvirus; Fowlpox virus; Turkeys; Vaccines. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177526/1/Outbreak-of-cutaneous-form-of-avian-poxvirus-disease.pdf
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Marc: |
LEADER 03240naa a2200373 a 4500
001 2091859
005 2018-05-23
008 2018 bl uuuu u00u1 u #d
100 1 $aFERREIRA, B. C.
245 $aOutbreak of cutaneous form of avian poxvirus disease in previously pox-vaccinated commercial turkeys.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Surto de bouba aviária na forma cutânea em perus comerciais previamente vacinados.
520 $aThis study describes an outbreak of avian poxvirus disease in previously pox-vaccinated turkeys in Brazil. The turkeys had suggestive gross lesions of cutaneous avian poxvirus in the skin of the head and cervical area without changes in the flock mortality rates. In the slaughterhouse, 30 carcasses were removed from the slaughter line to collect tissue from cutaneous lesions for histological analyses and characterization of the virus. The virus was identified by conventional polymerase chain reaction (PCR) and subsequent gene sequencing. Acanthosis, hyperkeratosis, and hydropic degeneration were seen on skin histopathology. Eosinophilic intracytoplasmic inclusion bodies (Bollinger) on keratinocytes were observed in 46.6% of the samples. Avian poxvirus DNA was detected on PCR in 83.3% of the total samples. PCR associated with histopathology had 93.3% of positivity for avian poxvirus. In the phylogenetic study, samples show 100% matching suggesting that the outbreak occurred by a single viral strain and was different from those strains affecting other wild birds such as canaries and sparrows. A single mutation (Adenine for Guanine) was detected in our study?s strain and in the strains of turkey, chickens, and vaccine strains published in GenBank. Also, when the sequence strain of the present study and sequences from GenBank of canarypox and sparrowpox strains were aligned, a Thymine was found replacing the Adenine or Guanine. The in ovo vaccination method as single-use in turkeys of this study apparently did not provide adequate protection against avianpox disease, but additional vaccination administered by wing-web when turkeys were 45-60 days old in the new flocks controlled the disease. In the subsequent year, new cases of this disease were not found. It was not possible to confirm the source of the virus strain, but infection with a field strain derived from chickens is one possibility, considering the poultry farm population in the area and biosecurity aspects. For wide characterization of avipoxvirus and differentiation among strains, the complete sequence of the viral genome is required.
650 $aAvipoxvirus
650 $aFowlpox virus
650 $aTurkeys
650 $aVaccines
650 $aBouba Aviaria
650 $aPeru
650 $aVacina
653 $aFpv167 gene
653 $aGene fpv167
653 $aPathology
653 $aPatologia
653 $aPox-vaccinated
653 $aSequenciamento
653 $aSequencing
700 1 $aECCO, R.
700 1 $aCOUTO, R. M.
700 1 $aCOELHO, H. E.
700 1 $aROSSI, D. A.
700 1 $aBELETTI, M. E.
700 1 $aSILVA, P. L.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 3, p. 417-424, março 2018
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