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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
26/11/2009 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, USP; FELIPE PERECIN, USP; NAIARA ZOCCAL SARAIVA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; WALT YAMAZAKI, UNESP; CLÁUDIA LIMA VERDE LEA, USP; FLÁVIO VIEIRA MEIRELLES, USP; JOAQUIM MANSANO GARCIA, UNESP. |
Título: |
Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009. |
DOI: |
https://doi.org/10.1016/j.anireprosci.2009.02.020 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages. |
Palavras-Chave: |
Artificial activation; Bovine; Embryonic development; Pronuclear transfer. |
Thesaurus Nal: |
beef cattle. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02365naa a2200289 a 4500 001 1576277 005 2023-03-15 008 2009 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.anireprosci.2009.02.020$2DOI 100 1 $aMEO, S. C. 245 $aKaryoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.$h[electronic resource] 260 $c2009 520 $aOoplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages. 650 $abeef cattle 653 $aArtificial activation 653 $aBovine 653 $aEmbryonic development 653 $aPronuclear transfer 700 1 $aFERREIRA, C. R. 700 1 $aPERECIN, F. 700 1 $aSARAIVA, N. Z. 700 1 $aTETZNER, T. A. D. 700 1 $aYAMAZAKI, W. 700 1 $aLEA, C. L. V. 700 1 $aMEIRELLES, F. V. 700 1 $aGARCIA, J. M. 773 $tAnimal Reproduction Science$gv. 116, n. 3-4, p. 381-385, dec. 2009.
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Embrapa Pecuária Sudeste (CPPSE) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
31/03/2011 |
Data da última atualização: |
30/09/2011 |
Tipo da produção científica: |
Folder/Folheto/Cartilha |
Autoria: |
COSTA, J. N. M. |
Afiliação: |
JOSE NILTON MEDEIROS COSTA, CPAF-RO. |
Título: |
Beauveria bassiana - Um fungo para combater a broca do café. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Porto Velho: Embrapa Rondônia, 2010. |
Descrição Física: |
Folder |
Idioma: |
Português |
Conteúdo: |
Esta publicação dedica-se a apresentar as principais informações para realização do controle leiteiro, dentre elas: vantagens do controle leiteiro; como executar o controle leiteiro; data e causa da secagem dos animais. |
Palavras-Chave: |
Broca do café. |
Thesagro: |
Café; Doença de Planta. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 00637nam a2200157 a 4500 001 1884399 005 2011-09-30 008 2010 bl uuuu u0uu1 u #d 100 1 $aCOSTA, J. N. M. 245 $aBeauveria bassiana - Um fungo para combater a broca do café. 260 $aPorto Velho: Embrapa Rondônia$c2010 300 $cFolder 520 $aEsta publicação dedica-se a apresentar as principais informações para realização do controle leiteiro, dentre elas: vantagens do controle leiteiro; como executar o controle leiteiro; data e causa da secagem dos animais. 650 $aCafé 650 $aDoença de Planta 653 $aBroca do café
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