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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
21/12/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. |
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARIANA CORTES BOITE, FIOCRUZ; SABINE WOHLRES-VIANA, UFJF; GUSTAVO BRUNO MOTA, UNB; RAQUEL VARELA SERAPIÃO, PESAGRO; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF. |
Título: |
Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Cryobiology, v. 63, n. 3, p. 256-262, 2011. |
DOI: |
https://doi.org/10.1016/j.cryobiol.2011.09.135 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. MenosThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-... Mostrar Tudo |
Palavras-Chave: |
Embryo. |
Thesaurus Nal: |
cryopreservation; osmosis; vitrification. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02924naa a2200265 a 4500 001 1910654 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.cryobiol.2011.09.135$2DOI 100 1 $aCAMARGO, L. S. de A. 245 $aOsmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro.$h[electronic resource] 260 $c2011 520 $aThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. 650 $acryopreservation 650 $aosmosis 650 $avitrification 653 $aEmbryo 700 1 $aBOITE, M. C. 700 1 $aWOHLRES-VIANA, S. 700 1 $aMOTA, G. B. 700 1 $aSERAPIÃO, R. V. 700 1 $aSÁ, W. F. de 700 1 $aVIANA, J. H. M. 700 1 $aNOGUEIRA, L. A. G. 773 $tCryobiology$gv. 63, n. 3, p. 256-262, 2011.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
06/12/1996 |
Data da última atualização: |
22/06/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CORREA, L. de A.; HAAG, H. P.; COSTA, J. L. D. |
Afiliação: |
LUCIANO DE ALMEIDA CORREA, CPPSE; HENRIQUE PAULO HAAG, USP-ESALQ; JOSE LADEIRA DA COSTA, CNPGL. |
Título: |
Níveis criticos de fósforo para o estabelecimento de Brachiaria decumbens Stapf., Brachiaria brizantha (Hochst.) Stapf. cv. Marandu e Panicum maximum Jacq., em latossolo amarelo, Álico. II. Experimento à campo. |
Ano de publicação: |
1992 |
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 29., 1992. Lavras. Anais... Lavras: SBZ, 1992. |
Páginas: |
p. 450. |
Idioma: |
Português |
Conteúdo: |
Determinar os níveis criticos de fósforo (P) no solo, pelos extratores de Mehlich I e Resina e na planta. |
Palavras-Chave: |
Adubação fosfatada; Fósforo na planta; Fósforo no solo; Gramineas forrageiras; Grass; Plant phosphorus; Soil phosphorus. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/120041/1/digitalizar0009.pdf
|
Marc: |
LEADER 00965nam a2200229 a 4500 001 1043360 005 2023-06-22 008 1992 bl uuuu u00u1 u #d 100 1 $aCORREA, L. de A. 245 $aNíveis criticos de fósforo para o estabelecimento de Brachiaria decumbens Stapf., Brachiaria brizantha (Hochst.) Stapf. cv. Marandu e Panicum maximum Jacq., em latossolo amarelo, Álico. II. Experimento à campo.$h[electronic resource] 260 $aIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 29., 1992. Lavras. Anais... Lavras: SBZ$c1992 300 $ap. 450. 520 $aDeterminar os níveis criticos de fósforo (P) no solo, pelos extratores de Mehlich I e Resina e na planta. 653 $aAdubação fosfatada 653 $aFósforo na planta 653 $aFósforo no solo 653 $aGramineas forrageiras 653 $aGrass 653 $aPlant phosphorus 653 $aSoil phosphorus 700 1 $aHAAG, H. P. 700 1 $aCOSTA, J. L. D.
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