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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
07/10/2008 |
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Data da última atualização: |
07/10/2008 |
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Autoria: |
NIVA, C. C.; LEE, J. M.; MYOHARA, M. |
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Título: |
PCNA as a cell proliferation marker in the oligochaete Enchytraeus japonensis. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
PCNA (proliferating cell nuclear antigen) is a protein involved in DNA replication expressed in
dividing cells which has been used as a cell proliferation marker for clinical studies of neoplastic
tissues in vertebrates, and also for developmental studies in several vertebrates and
invertebrates. Among invertebrates, PCNA has been detected by immunohistochemical methods
in several organisms, including the fragmenting oligochaete annelid E. japonensis. Despite the
apparent positive cross-reaction with commercially available antibodies, it was unknown whether
PCNA protein was really synthesized by E. japonensis. The objective of this study was to clarify
this question and verify whether PCNA could be used as a reliable molecular marker for
proliferative cells during E. japonensis regeneration, growth and embryo development at the
RNA level. For that purpose we cloned a cDNA encoding PCNA from E. japonensis by carrying
out degenerate PCR with primers designed from the conserved regions of known PCNAs using
a cDNA library of regenerating E. japonensis as the template. Two full-length cDNA sequences
with identical deduced amino acid sequence were obtained and the amino acid sequence
showed high similarity to known PCNAs of other organisms. We analyzed the gene expression
by RT-PCR and in situ hybridization in whole mounts (WISH) and paraffin sections in intact and
regenerating worms. The results showed a strong expression of pcna gene in the developing
growth zone of intact worms. In regenerating fragments, pcna expression was first detected in a
few cells in the vicinity of the amputated surface, and then clearly seen in cell agglomerates in
the blastema as regeneration progressed. Embryos also showed strong expression. We conclude
that pcna is a good cell proliferation marker for E. japonensis and may be a good biomarker to
study effects of stressors on developmental processes. MenosPCNA (proliferating cell nuclear antigen) is a protein involved in DNA replication expressed in
dividing cells which has been used as a cell proliferation marker for clinical studies of neoplastic
tissues in vertebrates, and also for developmental studies in several vertebrates and
invertebrates. Among invertebrates, PCNA has been detected by immunohistochemical methods
in several organisms, including the fragmenting oligochaete annelid E. japonensis. Despite the
apparent positive cross-reaction with commercially available antibodies, it was unknown whether
PCNA protein was really synthesized by E. japonensis. The objective of this study was to clarify
this question and verify whether PCNA could be used as a reliable molecular marker for
proliferative cells during E. japonensis regeneration, growth and embryo development at the
RNA level. For that purpose we cloned a cDNA encoding PCNA from E. japonensis by carrying
out degenerate PCR with primers designed from the conserved regions of known PCNAs using
a cDNA library of regenerating E. japonensis as the template. Two full-length cDNA sequences
with identical deduced amino acid sequence were obtained and the amino acid sequence
showed high similarity to known PCNAs of other organisms. We analyzed the gene expression
by RT-PCR and in situ hybridization in whole mounts (WISH) and paraffin sections in intact and
regenerating worms. The results showed a strong expression of pcna gene in the developing
growth zone of intact worms... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02578naa a2200145 a 4500
001 1315080
005 2008-10-07
008 2008 bl uuuu u00u1 u #d
100 1 $aNIVA, C. C.
245 $aPCNA as a cell proliferation marker in the oligochaete Enchytraeus japonensis.
260 $c2008
520 $aPCNA (proliferating cell nuclear antigen) is a protein involved in DNA replication expressed in dividing cells which has been used as a cell proliferation marker for clinical studies of neoplastic tissues in vertebrates, and also for developmental studies in several vertebrates and invertebrates. Among invertebrates, PCNA has been detected by immunohistochemical methods in several organisms, including the fragmenting oligochaete annelid E. japonensis. Despite the apparent positive cross-reaction with commercially available antibodies, it was unknown whether PCNA protein was really synthesized by E. japonensis. The objective of this study was to clarify this question and verify whether PCNA could be used as a reliable molecular marker for proliferative cells during E. japonensis regeneration, growth and embryo development at the RNA level. For that purpose we cloned a cDNA encoding PCNA from E. japonensis by carrying out degenerate PCR with primers designed from the conserved regions of known PCNAs using a cDNA library of regenerating E. japonensis as the template. Two full-length cDNA sequences with identical deduced amino acid sequence were obtained and the amino acid sequence showed high similarity to known PCNAs of other organisms. We analyzed the gene expression by RT-PCR and in situ hybridization in whole mounts (WISH) and paraffin sections in intact and regenerating worms. The results showed a strong expression of pcna gene in the developing growth zone of intact worms. In regenerating fragments, pcna expression was first detected in a few cells in the vicinity of the amputated surface, and then clearly seen in cell agglomerates in the blastema as regeneration progressed. Embryos also showed strong expression. We conclude that pcna is a good cell proliferation marker for E. japonensis and may be a good biomarker to study effects of stressors on developmental processes.
700 1 $aLEE, J. M.
700 1 $aMYOHARA, M.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Milho e Sorgo. |
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Data corrente: |
06/02/2024 |
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Data da última atualização: |
05/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RODRIGUES, B. M.; ALVARENGA, R. C.; LANNA, D. P. D.; OLIVEIRA, A. de L.; CERIBELIE, C.; CORREIA, B. S. B.; COLNAGO, L. A.; CARDOSO, D. R.; SOUZA, F. A. de; LOPES, L. S. |
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Afiliação: |
BARBARA MARTINS RODRIGUES, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RAMON COSTA ALVARENGA, CNPMS; DANTE PAZZANESE DUARTE LANNA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; AFONSO DE LIGUORI OLIVEIRA, UNIVERSIDADE DE SAO PAULO; CAROLINE CERIBELIE, UNIVERSIDADE DE SAO PAULO; BANNY SILVA BARBOSA CORREIA, UNIVERSIDADE DE SÃO PAULO; LUIZ ALBERTO COLNAGO, CNPDIA; DANIEL RODRIGUES CARDOSO, UNIVERSIDADE DE SAO PAULO; FERNANDO ANTÔNIO DE SOUZA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; LEANDRO SÂMIA LOPES, UNIVERSIDADE FEDERAL DE MINAS GERAIS. |
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Título: |
Aged-beef quality and fatty acid profile of subcutaneous fat from Nellore and Nellore × Angus crossbred young bulls finished in the feedlot with a high-lipid diet. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Animal Production Science, v. 64, n. 1, AN23165, 2024. |
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DOI: |
https://doi.org/10.1071/AN23165 |
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Idioma: |
Inglês |
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Conteúdo: |
Context. Although beef consumption has been linked to increased blood cholesterol, it has positive impacts on human health and provides essential nutrients of high biological value. Aims. This study investigated the effects of aging time on beef quality, the chemical composition of the Longissimus thoracis (LT) muscle, and the metabolite and fatty acid profile of the subcutaneous fat from young bulls finished in feedlot with a high-lipid diet. Methods. Fifty young bulls were divided into groups following their breed composition, Nellore (n = 25) and Angus × Nellore crossbred (n = 25), and com randomly assigned into four pens. The feedlot phase consisted of 105 days of feed, and the diet (80% concentrate) was formulated to meet or safely exceed the requirements of beef cattle to achieve an average daily gain of 1.6 kg/day. After slaughter, meat samples were collected between the 11th and 13th ribs 24 h postmortem. Steaks were aged for 1, 14, 28, and 42 days. Key results. No differences were observed for total weight loss, drip loss, and cooking loss between the genetic groups (P > 0.05). An increase in lipid oxidation was observed for TBARS in the LT beef and subcutaneous fat (P < 0.01). Shear force was influenced by breed and aging time (P < 0.01). There was a significant interaction between aging time and the parameters L* a*, b* c*, and h* (P < 0.01). The crossbred animals had greater (P < 0.01) concentrations of collagen, and Nellore had greater (P < 0.01) concentration of moisture in their LT beef. In total, 33 metabolites were identified in beef extract, and differences were observed in beef from both groups. Nellore animals had higher concentrations of polyunsaturated fatty acids and conjugated linoleic acid. Conclusions. The shear force, collagen concentration, and moisture were affected by breeds, while the aging time affected lipid oxidation, shear force, and colour parameters. Different breeds and a high-lipid diet modified the metabolite profile of meat and the profile of subcutaneous fat. Implications. These findings suggest that genetic manipulation could potentially be used to improve beef quality. MenosContext. Although beef consumption has been linked to increased blood cholesterol, it has positive impacts on human health and provides essential nutrients of high biological value. Aims. This study investigated the effects of aging time on beef quality, the chemical composition of the Longissimus thoracis (LT) muscle, and the metabolite and fatty acid profile of the subcutaneous fat from young bulls finished in feedlot with a high-lipid diet. Methods. Fifty young bulls were divided into groups following their breed composition, Nellore (n = 25) and Angus × Nellore crossbred (n = 25), and com randomly assigned into four pens. The feedlot phase consisted of 105 days of feed, and the diet (80% concentrate) was formulated to meet or safely exceed the requirements of beef cattle to achieve an average daily gain of 1.6 kg/day. After slaughter, meat samples were collected between the 11th and 13th ribs 24 h postmortem. Steaks were aged for 1, 14, 28, and 42 days. Key results. No differences were observed for total weight loss, drip loss, and cooking loss between the genetic groups (P > 0.05). An increase in lipid oxidation was observed for TBARS in the LT beef and subcutaneous fat (P < 0.01). Shear force was influenced by breed and aging time (P < 0.01). There was a significant interaction between aging time and the parameters L* a*, b* c*, and h* (P < 0.01). The crossbred animals had greater (P < 0.01) concentrations of collagen, and Nellore had greater (P < 0.01) concentration o... Mostrar Tudo |
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Palavras-Chave: |
Composição da carne; Metabolômica. |
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Thesagro: |
Ácido Linoléico; Bovino; Carne; Confinamento; Genética Animal; Lipídio; Nutrição Animal. |
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Thesaurus NAL: |
Animal nutrition; Beef; Conjugated linoleic acid; Feedlots; Genetics; Lipids; Meat composition; Metabolomics. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03468naa a2200445 a 4500
001 2161715
005 2024-03-05
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/AN23165$2DOI
100 1 $aRODRIGUES, B. M.
245 $aAged-beef quality and fatty acid profile of subcutaneous fat from Nellore and Nellore × Angus crossbred young bulls finished in the feedlot with a high-lipid diet.$h[electronic resource]
260 $c2024
520 $aContext. Although beef consumption has been linked to increased blood cholesterol, it has positive impacts on human health and provides essential nutrients of high biological value. Aims. This study investigated the effects of aging time on beef quality, the chemical composition of the Longissimus thoracis (LT) muscle, and the metabolite and fatty acid profile of the subcutaneous fat from young bulls finished in feedlot with a high-lipid diet. Methods. Fifty young bulls were divided into groups following their breed composition, Nellore (n = 25) and Angus × Nellore crossbred (n = 25), and com randomly assigned into four pens. The feedlot phase consisted of 105 days of feed, and the diet (80% concentrate) was formulated to meet or safely exceed the requirements of beef cattle to achieve an average daily gain of 1.6 kg/day. After slaughter, meat samples were collected between the 11th and 13th ribs 24 h postmortem. Steaks were aged for 1, 14, 28, and 42 days. Key results. No differences were observed for total weight loss, drip loss, and cooking loss between the genetic groups (P > 0.05). An increase in lipid oxidation was observed for TBARS in the LT beef and subcutaneous fat (P < 0.01). Shear force was influenced by breed and aging time (P < 0.01). There was a significant interaction between aging time and the parameters L* a*, b* c*, and h* (P < 0.01). The crossbred animals had greater (P < 0.01) concentrations of collagen, and Nellore had greater (P < 0.01) concentration of moisture in their LT beef. In total, 33 metabolites were identified in beef extract, and differences were observed in beef from both groups. Nellore animals had higher concentrations of polyunsaturated fatty acids and conjugated linoleic acid. Conclusions. The shear force, collagen concentration, and moisture were affected by breeds, while the aging time affected lipid oxidation, shear force, and colour parameters. Different breeds and a high-lipid diet modified the metabolite profile of meat and the profile of subcutaneous fat. Implications. These findings suggest that genetic manipulation could potentially be used to improve beef quality.
650 $aAnimal nutrition
650 $aBeef
650 $aConjugated linoleic acid
650 $aFeedlots
650 $aGenetics
650 $aLipids
650 $aMeat composition
650 $aMetabolomics
650 $aÁcido Linoléico
650 $aBovino
650 $aCarne
650 $aConfinamento
650 $aGenética Animal
650 $aLipídio
650 $aNutrição Animal
653 $aComposição da carne
653 $aMetabolômica
700 1 $aALVARENGA, R. C.
700 1 $aLANNA, D. P. D.
700 1 $aOLIVEIRA, A. de L.
700 1 $aCERIBELIE, C.
700 1 $aCORREIA, B. S. B.
700 1 $aCOLNAGO, L. A.
700 1 $aCARDOSO, D. R.
700 1 $aSOUZA, F. A. de
700 1 $aLOPES, L. S.
773 $tAnimal Production Science$gv. 64, n. 1, AN23165, 2024.
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